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SCN resistance requires both the <jats:italic>GmSHMT08<\/jats:italic> and the <jats:italic>GmSNAP18<\/jats:italic> in \u2018Peking\u2019\u2010type resistance<jats:italic>.<\/jats:italic> Here, we describe the molecular interaction between GmSHMT08 and GmSNAP18, which is potentiated by a pathogenesis\u2010related protein GmPR08\u2010Bet VI. Like <jats:italic>GmSNAP18<\/jats:italic> and <jats:italic>GmSHMT08<\/jats:italic>, <jats:italic>GmPR08\u2010Bet VI<\/jats:italic> expression was induced in response to SCN and its overexpression decreased SCN cysts by 65% in infected transgenic soybean roots. Overexpression of GmPR08\u2010Bet VI did not have an effect on SCN resistance when the two cytokinin\u2010binding sites in GmPR08\u2010Bet VI were mutated, indicating a new role of GmPR08\u2010Bet VI in SCN resistance. GmPR08\u2010Bet VI was mapped to a QTL for resistance to SCN using different mapping populations. GmSHMT08, GmSNAP18 and GmPR08\u2010Bet VI localize to the cytosol and plasma membrane. <jats:italic>GmSNAP18<\/jats:italic> expression and localization hyper\u2010accumulated at the plasma membrane and was specific to the root cells surrounding the nematode in SCN\u2010resistant soybeans. Genes encoding key components of the salicylic acid signalling pathway were induced under SCN infection. <jats:italic>GmSNAP18<\/jats:italic> and <jats:italic>GmPR08\u2010Bet VI<\/jats:italic> were also induced under salicylic acid and cytokinin exogenous treatments, while <jats:italic>GmSHMT08<\/jats:italic> was induced only when the resistant <jats:italic>GmSNAP18<\/jats:italic> was present, pointing to the presence of a molecular crosstalk between SCN\u2010resistant genes and defence genes. Expression analysis of <jats:italic>GmSHMT08<\/jats:italic> and <jats:italic>GmSNAP18<\/jats:italic> identified the need of a minimum expression requirement to trigger the SCN resistance reaction. These results provide insight into a new response mechanism towards plant nematode resistance involving haplotype compatibility, gene dosage and hormone signalling.<\/jats:p>","DOI":"10.1111\/pbi.13343","type":"journal-article","created":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T06:15:46Z","timestamp":1579587346000},"page":"1810-1829","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["A pathogenesis\u2010related protein GmPR08\u2010Bet VI promotes a molecular interaction between the GmSHMT08 and GmSNAP18 in resistance to <i>Heterodera glycines<\/i>"],"prefix":"10.1111","volume":"18","author":[{"given":"Naoufal","family":"Lakhssassi","sequence":"first","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarbottam","family":"Piya","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadia","family":"Bekal","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiming","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhou","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Bergounioux","sequence":"additional","affiliation":[{"name":"INRA Institute of Plant Sciences Paris\u2010Saclay (IPS2) CNRS Universit\u00e9 Paris\u2010Sud Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Long","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonas","family":"Meksem","sequence":"additional","affiliation":[{"name":"Duke University Durham NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aicha","family":"Lakhssassi","sequence":"additional","affiliation":[{"name":"Faculty of Sciences and Technologies University of Lorraine Nancy France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Jones","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"My Abdelmajid","family":"Kassem","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Fayetteville State University Fayetteville NC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Moussa","family":"Benhamed","sequence":"additional","affiliation":[{"name":"INRA Institute of Plant Sciences Paris\u2010Saclay (IPS2) CNRS Universit\u00e9 Paris\u2010Sud Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhafid","family":"Bendahmane","sequence":"additional","affiliation":[{"name":"INRA Institute of Plant Sciences Paris\u2010Saclay (IPS2) CNRS Universit\u00e9 Paris\u2010Sud Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kris","family":"Lambert","sequence":"additional","affiliation":[{"name":"Department of Crop Sciences University of Illinois Urbana IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adnane","family":"Boualem","sequence":"additional","affiliation":[{"name":"INRA Institute of Plant Sciences Paris\u2010Saclay (IPS2) CNRS Universit\u00e9 Paris\u2010Sud Orsay France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarek","family":"Hewezi","sequence":"additional","affiliation":[{"name":"Department of Plant Sciences University of Tennessee Knoxville TN USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9557-0051","authenticated-orcid":false,"given":"Khalid","family":"Meksem","sequence":"additional","affiliation":[{"name":"Department of Plant, Soil and Agricultural Systems Southern Illinois University Carbondale IL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,2,5]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"725","article-title":"Should I fight or should I grow now? 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