{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T08:49:52Z","timestamp":1791881392615,"version":"4.3.4"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>MYB transcription factors (TFs) have been reported to regulate the biosynthesis of secondary metabolites, as well as to mediate plant adaption to abiotic stresses, including drought. However, the roles of MYB TFs in regulating plant architecture and yield potential remain poorly understood. Here, we studied the roles of the dehydration\u2010inducible <jats:italic>GmMYB14<\/jats:italic> gene in regulating plant architecture, high\u2010density yield and drought tolerance through the brassinosteroid (BR) pathway in soybean. GmMYB14 was shown to localize to nucleus and has a transactivation activity. Stable <jats:italic>GmMYB14<\/jats:italic>\u2010overexpressing (<jats:italic>GmMYB14\u2010OX<\/jats:italic>) transgenic soybean plants displayed a semi\u2010dwarfism and compact plant architecture associated with decreased cell size, resulting in a decrease in plant height, internode length, leaf area, leaf petiole length and leaf petiole angle, and improved yield in high density under field conditions. Results of the transcriptome sequencing suggested the involvement of BRs in regulating <jats:italic>GmMYB14\u2010OX<\/jats:italic> plant architecture. Indeed, <jats:italic>GmMYB14\u2010OX<\/jats:italic> plants showed reduced endogenous BR contents, while exogenous application of brassinolide could partly rescue the phenotype of <jats:italic>GmMYB14\u2010OX<\/jats:italic> plants. Furthermore, GmMYB14 was shown to directly bind to the promoter of <jats:italic>GmBEN1<\/jats:italic> and up\u2010regulate its expression, leading to reduced BR content in <jats:italic>GmMYB14\u2010OX<\/jats:italic> plants. <jats:italic>GmMYB14\u2010OX<\/jats:italic> plants also displayed improved drought tolerance under field conditions. <jats:italic>GmBEN1<\/jats:italic> expression was also up\u2010regulated in the leaves of <jats:italic>GmMYB14\u2010OX<\/jats:italic> plants under polyethylene glycol treatment, indicating that the <jats:italic>GmBEN1<\/jats:italic>\u2010mediated reduction in BR level under stress also contributed to drought\/osmotic stress tolerance of the transgenic plants. Our findings provided a strategy for stably increasing high\u2010density yield and drought tolerance in soybean using a single TF\u2010encoding gene.<\/jats:p>","DOI":"10.1111\/pbi.13496","type":"journal-article","created":{"date-parts":[[2020,10,24]],"date-time":"2020-10-24T04:04:32Z","timestamp":1603512272000},"page":"702-716","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":193,"title":["Overexpression of <i>GmMYB14<\/i> improves high\u2010density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0607-6263","authenticated-orcid":false,"given":"Limiao","family":"Chen","sequence":"first","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Oil Crops Ministry of Agriculture and Rural Affairs Oil Crops Research Institute Chinese Academy of Agricultural 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