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Current control measures involve the use of chemical insecticides and transgenic maize expressing <jats:italic>Bacillus thuringiensis<\/jats:italic> (<jats:italic>Bt<\/jats:italic>) toxins. Although additional transgenes have confirmed insecticidal activity, limited research has been conducted in maize, at least partially due to the technical difficulty of maize transformation. Here, we describe implementation of a sugarcane mosaic virus (SCMV) vector for rapidly testing the efficacy of both endogenous maize genes and heterologous genes from other organisms for the control of <jats:italic>S.\u00a0frugiperda<\/jats:italic> in maize. Four categories of proteins were tested using the SCMV vector: (i) maize defence signalling proteins: peptide elicitors (Pep1 and Pep3) and jasmonate acid conjugating enzymes (JAR1a and JAR1b); (ii) maize defensive proteins: the previously identified ribosome\u2010inactivating protein (RIP2) and maize proteinase inhibitor (MPI), and two proteins with predicted but unconfirmed anti\u2010insect activities, an antimicrobial peptide (AMP) and a lectin (JAC1); (iii) lectins from other plant species: <jats:italic>Allium cepa<\/jats:italic> agglutinin (ACA) and <jats:italic>Galanthus<\/jats:italic> <jats:italic>nivalis<\/jats:italic> agglutinin (GNA); and (iv) scorpion and spider toxins: peptides from <jats:italic>Urodacus yaschenkoi<\/jats:italic> (UyCT3 and UyCT5) and <jats:italic>Hadronyche versuta<\/jats:italic> (Hvt). In most cases, <jats:italic>S.\u00a0frugiperda<\/jats:italic> larval growth was reduced by transient SCMV\u2010mediated overexpression of genes encoding these proteins. Additionally, experiments with a subset of the SCMV\u2010expressed genes showed effectiveness against two aphid species, <jats:italic>Rhopalosiphum maidis<\/jats:italic> (corn leaf aphid) and <jats:italic>Myzus persicae<\/jats:italic> (green peach aphid). Together, these results demonstrate that SCMV vectors are a rapid screening method for testing the efficacy and insecticidal activity of candidate genes in maize.<\/jats:p>","DOI":"10.1111\/pbi.13585","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T06:47:10Z","timestamp":1616654830000},"page":"1713-1724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["A sugarcane mosaic virus vector for rapid <i>in planta<\/i> screening of proteins that inhibit the growth of insect herbivores"],"prefix":"10.1111","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4108-8320","authenticated-orcid":false,"given":"Seung Ho","family":"Chung","sequence":"first","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahdiyeh","family":"Bigham","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan R.","family":"Lappe","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology Iowa State University Ames IA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and The Genome Center College of Biological Sciences University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ugrappa","family":"Nagalakshmi","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and The Genome Center College of Biological Sciences University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven A.","family":"Whitham","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology and Microbiology Iowa State University Ames IA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Savithramma P.","family":"Dinesh\u2010Kumar","sequence":"additional","affiliation":[{"name":"Department of Plant Biology and The Genome Center College of Biological Sciences University of California Davis CA USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9675-934X","authenticated-orcid":false,"given":"Georg","family":"Jander","sequence":"additional","affiliation":[{"name":"Boyce Thompson Institute for Plant Research Ithaca NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,4,8]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200900514"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/1541-4337.12192"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0710338104"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erv180"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh243"},{"key":"e_1_2_8_7_1","first-page":"e62277","article-title":"Direct agroinoculation of maize seedlings by injection with recombinant foxtail mosaic virus and sugarcane mosaic virus infectious clones","volume":"168","author":"Beernink B.M.","year":"2021","journal-title":"J. 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