{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:30:15Z","timestamp":1790634615856,"version":"4.1.0"},"reference-count":77,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,8,19]],"date-time":"2022-08-19T00:00:00Z","timestamp":1660867200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31901881"],"award-info":[{"award-number":["31901881"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31871979"],"award-info":[{"award-number":["31871979"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Aphids secrete diverse repertoires of salivary effectors into host plant cells to promote infestation by modulating plant defence. The greenbug <jats:italic>Schizaphis graminum<\/jats:italic> is an important cereal aphid worldwide. However, the secreted effectors of <jats:italic>S. graminum<\/jats:italic> are still uncharacterized. Here, 76 salivary proteins were identified from the watery saliva of <jats:italic>S. graminum<\/jats:italic> using transcriptome and proteome analyses. Among them, a putative salivary effector Sg2204 was significantly up\u2010regulated during aphid feeding stages, and transient overexpression of Sg2204 in <jats:italic>Nicotiana benthamiana<\/jats:italic> inhibited cell death induced by BAX or INF1. Delivering Sg2204 into wheat via the type III secretion system of <jats:italic>Pseudomonas fluorescens<\/jats:italic> EtAnH suppressed pattern\u2010triggered immunity (PTI)\u2010associated callose deposition. The transcript levels of jasmonic acid (JA)\u2010 and salicylic acid (SA)\u2010associated defence genes of wheat were significantly down\u2010regulated, and the contents of both JA and SA were also significantly decreased after delivery of Sg2204 into wheat leaves. Additionally, feeding on wheat expressing Sg2204 significantly increased the weight and fecundity of <jats:italic>S. graminum<\/jats:italic> and promoted aphid phloem feeding. <jats:italic>Sg2204<\/jats:italic> was efficiently silenced via spray\u2010based application of the nanocarrier\u2010mediated transdermal dsRNA delivery system. Moreover, <jats:italic>Sg2204<\/jats:italic>\u2010silenced aphids induced a stronger wheat defence response and resulted in negative impacts on aphid feeding behaviour, survival and fecundity. Silencing of <jats:italic>Sg2204<\/jats:italic> homologues from four aphid species using nanocarrier\u2010delivered dsRNA also significantly reduced aphid performance on host plants. Thus, our study characterized the salivary effector Sg2204 of <jats:italic>S. graminum<\/jats:italic> involved in promoting host susceptibility by suppressing wheat defence, which can also be regarded as a promising RNAi target for aphid control.<\/jats:p>","DOI":"10.1111\/pbi.13900","type":"journal-article","created":{"date-parts":[[2022,7,29]],"date-time":"2022-07-29T07:31:31Z","timestamp":1659079891000},"page":"2187-2201","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":71,"title":["The salivary effector protein Sg2204 in the greenbug <i>Schizaphis graminum<\/i> suppresses wheat defence and is essential for enabling aphid feeding on host plants"],"prefix":"10.1111","volume":"20","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4134-2405","authenticated-orcid":false,"given":"Yong","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobei","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Francis","sequence":"additional","affiliation":[{"name":"Functional and Evolutionary Entomology, Gembloux Agro\u2010Bio Tech University of Li\u00e8ge  Gembloux Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haicui","family":"Xie","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology Hebei Normal University of Science and Technology  Qinhuangdao City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jia","family":"Fan","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"},{"name":"Department of Entomology College of Plant Protection China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"},{"name":"College of Agronomy and Biotechnology Hebei Normal University of Science and Technology  Qinhuangdao City China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection, Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,8,19]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12322"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2021.711896"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cropro.2016.06.008"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/aesa\/76.6.964"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-06-12-0144-FI"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab383"},{"key":"e_1_2_8_8_1","volume-title":"Aphids on the World's Crops: An Identification and Information Guide","author":"Blackman R.L.","year":"2000"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1001216"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.2006.00443.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.730"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-020-02527-9"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1407687111"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.15475"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13610"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2021.102029"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1098\/rstb.2018.0314"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2009.02019.x"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.7.5.589"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa015"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2012.09.003"},{"key":"e_1_2_8_22_1","unstructured":"Drurey C. Mathers T.C. Prince D.C. Wilson C. Caceres\u2010Moreno C. Mugford S.T.andHogenhout S.A.(2019)Chemosensory proteins in the CSP4 clade evolved as plant immunity suppressors before two suborders of plant\u2010feeding hemipteran insects diverged.BioRxiv 173278."},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-01-14-0018-R"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eraa043"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007485321000237"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.71.1.182"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3119"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2020.09.020"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2583.2008.00790.x"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2011.05.003"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1094\/Phyto-86-704"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17620"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-18240-y"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05286"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.040204.135944"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.016980"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.1c07046"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nantod.2022.101452"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/acssuschemeng.9b00004"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-19-0655"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340\u2010022\u201001485\u20105"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90282-8"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1999.01403.x"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-08-16-0168-R"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0708958105"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1673\/031.006.3801"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-23-8-0991"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2013.12.005"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2021.06.005"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025709"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-07-12-0172-FI"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1570-7458.1994.tb01813.x"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13589"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2013.08.002"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-05-12-0119-FI"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-05-13-0156-R"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-ento-010715-023851"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erz152"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13067"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2012.02.010"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-07-13-0187-FI"},{"key":"e_1_2_8_63_1","first-page":"e1840","article-title":"Functional evaluation of proteins in watery and gel saliva of aphids","volume":"7","author":"Bel A.J.","year":"2016","journal-title":"Front. Plant Sci."},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2021.104878"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erj089"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err226"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1714990116"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-13487-6"},{"key":"e_1_2_8_69_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10340-019-01157-x"},{"key":"e_1_2_8_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cois.2018.01.003"},{"key":"e_1_2_8_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-010-0285-x"},{"key":"e_1_2_8_72_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.7b03141"},{"key":"e_1_2_8_73_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-019-2148-5"},{"key":"e_1_2_8_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-020-6743-5"},{"key":"e_1_2_8_75_1","doi-asserted-by":"publisher","DOI":"10.1111\/1744-7917.12953"},{"key":"e_1_2_8_76_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01294"},{"key":"e_1_2_8_77_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.103.028324"},{"key":"e_1_2_8_78_1","doi-asserted-by":"publisher","DOI":"10.1038\/nplants.2015.206"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.13900","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.13900","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.13900","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T04:41:50Z","timestamp":1692679310000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.13900"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,19]]},"references-count":77,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2022,11]]}},"alternative-id":["10.1111\/pbi.13900"],"URL":"https:\/\/doi.org\/10.1111\/pbi.13900","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,19]]},"assertion":[{"value":"2022-03-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-19","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}