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We further developed this mAb into a chimeric human IgG and recombinantly expressed it in plants to produce a mAb with human\u2010like, highly homogenous N\u2010linked glycans that has potential to impart greater potency and safety as a therapeutic. The epitope of 11D7 was mapped by competitive binding with well\u2010characterized mAbs, suggesting that it is a Class 4 RBD\u2010binding mAb that binds to the RBD outside the ACE2 binding site. Of note, 11D7 maintains recognition against the B.1.1.529 (Omicron) RBD, as well neutralizing activity. We also provide evidence that this novel mAb may be useful in providing additional synergy to established antibody cocktails, such as Evusheld\u2122 containing the antibodies tixagevimab and cilgavimab, against the Omicron variant. Taken together, 11D7 is a unique mAb that neutralizes SARS\u2010CoV\u20102 through a mechanism that is not typical among developed therapeutic mAbs and by being produced in \u0394XFT <jats:italic>Nicotiana benthamiana<\/jats:italic> plants, highlights the potential of plants to be an economic and safety\u2010friendly alternative platform for generating mAbs to address the evolving SARS\u2010CoV\u20102 crisis.<\/jats:p>","DOI":"10.1111\/pbi.13970","type":"journal-article","created":{"date-parts":[[2022,11,20]],"date-time":"2022-11-20T16:13:55Z","timestamp":1668960835000},"page":"549-559","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["A novel plant\u2010made monoclonal antibody enhances the synergetic potency of an antibody cocktail against the <scp>SARS\u2010CoV<\/scp>\u20102 Omicron variant"],"prefix":"10.1111","volume":"21","author":[{"given":"Collin","family":"Jugler","sequence":"first","affiliation":[{"name":"The Biodesign Institute Arizona State University  Tempe Arizona USA"},{"name":"School of Life Sciences Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiyan","family":"Sun","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katherine","family":"Nguyen","sequence":"additional","affiliation":[{"name":"School of Molecular Sciences Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roman","family":"Palt","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitchell","family":"Felder","sequence":"additional","affiliation":[{"name":"Halberd Corporation  Jackson Center Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herta","family":"Steinkellner","sequence":"additional","affiliation":[{"name":"Department of Applied Genetics and Cell Biology University of Natural Resources and Life Sciences  Vienna Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1498-7013","authenticated-orcid":false,"given":"Qiang","family":"Chen","sequence":"additional","affiliation":[{"name":"The Biodesign Institute Arizona State University  Tempe Arizona USA"},{"name":"School of Life Sciences Arizona State University  Tempe Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,16]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/md19080418"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.14364"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2852-1"},{"key":"e_1_2_9_5_1","first-page":"93","article-title":"What is synergy?","volume":"41","author":"Berenbaum M.C.","year":"1989","journal-title":"Pharmacol. 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