{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T23:01:45Z","timestamp":1778886105975,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,8,18]],"date-time":"2023-08-18T00:00:00Z","timestamp":1692316800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011929","name":"Foundation for Food and Agriculture Research","doi-asserted-by":"publisher","award":["FF\u2010NIA21\u20130000000017"],"award-info":[{"award-number":["FF\u2010NIA21\u20130000000017"]}],"id":[{"id":"10.13039\/100011929","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012009","name":"United Soybean Board","doi-asserted-by":"publisher","award":["USB 1920\u2010152\u20100131\u2010D"],"award-info":[{"award-number":["USB 1920\u2010152\u20100131\u2010D"]}],"id":[{"id":"10.13039\/100012009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2023,12]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Hybrid breeding for increased vigour has been used for over a century to boost agricultural outputs without requiring higher inputs. While this approach has led to some of the most substantial gains in crop productivity, breeding barriers have fundamentally limited soybean (\n                    <jats:italic>Glycine max<\/jats:italic>\n                    ) from reaping the benefits of hybrid vigour. Soybean flowers self\u2010pollinate prior to opening and thus are not readily amenable to outcrossing. In this study, we demonstrate that the barnase\/barstar male sterility\/rescue system can be used in soybean to produce hybrid seeds. By expressing the cytotoxic ribonuclease, barnase, under a tapetum\u2010specific promoter in soybean anthers, we are able to completely block pollen maturation, creating male sterile plants. We show that fertility can be rescued in the F1 generation of these barnase\u2010expressing lines when they are crossed with pollen from plants that express the barnase inhibitor, barstar. Importantly, we found that the successful rescue of male fertility is dependent on the relative dosage of barnase and barstar. When barnase and barstar were expressed under the same tapetum\u2010specific promoter, the F1 offspring remained male sterile. When we expressed barstar under a relatively stronger promoter than barnase, we were able to achieve a successful rescue of male fertility in the F1 generation. This work demonstrates the successful implementation of a biotechnology approach to produce fertile hybrid offspring in soybean.\n                  <\/jats:p>","DOI":"10.1111\/pbi.14155","type":"journal-article","created":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T14:18:17Z","timestamp":1692627497000},"page":"2585-2596","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Introduction of barnase\/barstar in soybean produces a rescuable male sterility system for hybrid breeding"],"prefix":"10.1111","volume":"21","author":[{"given":"Nicole","family":"Szeluga","sequence":"first","affiliation":[{"name":"Plant Biology Section, School of Integrative Plant Science Cornell University  Ithaca NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4669-6632","authenticated-orcid":false,"given":"Patricia","family":"Baldrich","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St. Louis MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"DelPercio","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St. Louis MO USA"},{"name":"Division of Plant Sciences and Technology University of Missouri \u2013 Columbia  Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3436-6097","authenticated-orcid":false,"given":"Blake C.","family":"Meyers","sequence":"additional","affiliation":[{"name":"Donald Danforth Plant Science Center  St. Louis MO USA"},{"name":"Division of Plant Sciences and Technology University of Missouri \u2013 Columbia  Columbia MO USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8269-8240","authenticated-orcid":false,"given":"Margaret H.","family":"Frank","sequence":"additional","affiliation":[{"name":"Plant Biology Section, School of Integrative Plant Science Cornell University  Ithaca NY USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,8,18]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"SoyStats 2022","author":"American Soybean Association","year":"2022"},{"key":"e_1_2_7_3_1","volume-title":"\u201cOil crops outlook: january 2023.\u201d OCS\u201023a","author":"Ates A.M.","year":"2023"},{"key":"e_1_2_7_4_1","volume-title":"\u201cOil crops outlook: february 2023.\u201dOCS\u201023b","author":"Ates A.M.","year":"2023"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0523.2006.01191.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.9.9.1527"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-016-1057-7"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:MOLB.0000038002.45312.08"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-006-0274-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13592-017-0532-4"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci2006.03.0156"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbr.12473"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1079\/9780851994482.0000"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.101.4.1295"},{"key":"e_1_2_7_15_1","volume-title":"\u201cUSDA agricultural projections to 2031.\u201d OCE\u20102022\u201001","author":"Dohlman E.","year":"2022"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/sb4001504"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1975.0011183X001500060008x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.2135\/cropsci1975.0011183X001500010025x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jee\/71.4.601"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10681-014-1103-7"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2021.03.013"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(88)90568-2"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(89)90104-7"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(01)41179-7"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2199-10-93"},{"key":"e_1_2_7_26_1","first-page":"46","article-title":"Development of transgenic barstar lines and identification of a male sterile (barnase)\/restorer (barstar) combination for heterosis\u00a0breeding in indian oilseed mustard (Brassica Juncea)","volume":"82","author":"Jagannath A.","year":"2002","journal-title":"Curr. 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