{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T15:52:43Z","timestamp":1789573963499,"version":"build-2803163510"},"reference-count":54,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T00:00:00Z","timestamp":1697673600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Barley yellow dwarf viruses (BYDVs) cause widespread damage to global cereal crops. Here we report a novel strategy for elevating resistance to BYDV infection. The 17K protein, a potent virulence factor conserved in BYDVs, interacted with barley IMP\u2010\u03b11 and \u2010\u03b12 proteins that are nuclear transport receptors. Consistently, a nuclear localization signal was predicted in 17K, which was found essential for 17K to be transported into the nucleus and to interact with IMP\u2010\u03b11 and \u2010\u03b12. Reducing <jats:italic>HvIMP\u2010\u03b11<\/jats:italic> and <jats:italic>\u2010\u03b12<\/jats:italic> expression by gene silencing attenuated BYDV\u2010elicited dwarfism, accompanied by a lowered nuclear accumulation of 17K. Among the eight common wheat CRISPR mutants with two to four <jats:italic>TaIMP\u2010\u03b11<\/jats:italic> and <jats:italic>\u2010\u03b12<\/jats:italic> genes mutated, the triple mutant \u03b11<jats:sup>aaBBDD<\/jats:sup>\/\u03b12<jats:sup>AAbbdd<\/jats:sup> and the tetra\u2010mutant \u03b11<jats:sup>aabbdd<\/jats:sup>\/\u03b12<jats:sup>AAbbDD<\/jats:sup> displayed strong BYDV resistance without negative effects on plant growth under field conditions. The BYDV resistance exhibited by \u03b11<jats:sup>aaBBDD<\/jats:sup>\/\u03b12<jats:sup>AAbbdd<\/jats:sup> and \u03b11<jats:sup>aabbdd<\/jats:sup>\/\u03b12<jats:sup>AAbbDD<\/jats:sup> was correlated with decreased nuclear accumulation of 17K and lowered viral proliferation in infected plants. Our work uncovers the function of host IMP\u2010\u03b1 proteins in BYDV pathogenesis and generates the germplasm valuable for breeding BYDV\u2010resistant wheat. Appropriate reduction of <jats:italic>IMP\u2010\u03b1<\/jats:italic> gene expression may be broadly useful for enhancing antiviral resistance in agricultural crops and other economically important organisms.<\/jats:p>","DOI":"10.1111\/pbi.14204","type":"journal-article","created":{"date-parts":[[2023,10,19]],"date-time":"2023-10-19T14:35:45Z","timestamp":1697726145000},"page":"572-586","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Appropriate reduction of <i>importin\u2010\u03b1<\/i> gene expression enhances yellow dwarf disease resistance in common wheat"],"prefix":"10.1111","volume":"22","author":[{"given":"Lina","family":"Wang","sequence":"first","affiliation":[{"name":"National Wheat Engineering Research Center, College of Agronomy Henan Agricultural University  Zhengzhou Henan China"},{"name":"State Key Laboratory of Wheat and Maize Crop Science, Center for Crop Genome Engineering, College of Agronomy Henan 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