{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T16:36:37Z","timestamp":1788021397476,"version":"build-2784847793"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T00:00:00Z","timestamp":1704931200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172558"],"award-info":[{"award-number":["32172558"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32030092"],"award-info":[{"award-number":["32030092"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Cytoplasmic male sterility (CMS), encoded by the mitochondrial open reading frames (\n                    <jats:italic>ORFs<\/jats:italic>\n                    ), has long been used to economically produce crop hybrids. However, the utilization of CMS also hinders the exploitation of sterility and fertility variation in the absence of a restorer line, which in turn narrows the genetic background and reduces biodiversity. Here, we used a mitochondrial targeted transcription activator\u2010like effector nuclease (mitoTALENs) to knock out\n                    <jats:italic>ORF138<\/jats:italic>\n                    from the\n                    <jats:italic>Ogura<\/jats:italic>\n                    CMS broccoli hybrid. The knockout was confirmed by the amplification and re\u2010sequencing read mapping to the mitochondrial genome. As a result, knockout of\n                    <jats:italic>ORF138<\/jats:italic>\n                    restored the fertility of the CMS hybrid, and simultaneously manifested a cold\u2010sensitive male sterility.\n                    <jats:italic>ORF138<\/jats:italic>\n                    depletion is stably inherited to the next generation, allowing for direct use in the breeding process. In addition, we proposed a highly reliable and cost\u2010effective toolkit to accelerate the life cycle of fertile lines from CMS\u2010derived broccoli hybrids. By applying the k\u2010mean clustering and interaction network analysis, we identified the central gene networks involved in the fertility restoration and cold\u2010sensitive male sterility. Our study enables mitochondrial genome editing via mitoTALENs in\n                    <jats:italic>Brassicaceae<\/jats:italic>\n                    vegetable crops and provides evidence that the sex production machinery and its temperature\u2010responsive ability are regulated by the mitochondria.\n                  <\/jats:p>","DOI":"10.1111\/pbi.14268","type":"journal-article","created":{"date-parts":[[2024,1,12]],"date-time":"2024-01-12T02:10:30Z","timestamp":1705025430000},"page":"1325-1334","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Editing of\n                    <i>ORF138<\/i>\n                    restores fertility of\n                    <i>Ogura<\/i>\n                    cytoplasmic male sterile broccoli via\n                    <scp>mitoTALENs<\/scp>"],"prefix":"10.1111","volume":"22","author":[{"given":"Fengyuan","family":"Xu","sequence":"first","affiliation":[{"name":"Hainan Institute, Zhejiang University Yazhou Bay Science and Technology City  Sanya China"},{"name":"Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tongbing","family":"Su","sequence":"additional","affiliation":[{"name":"Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hainan Institute, Zhejiang University Yazhou Bay Science and Technology City  Sanya China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Qiu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaodong","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape Architecture Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuya","family":"Koizuka","sequence":"additional","affiliation":[{"name":"College of Agriculture Tamagawa University  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010ichi","family":"Arimura","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongyuan","family":"Hu","sequence":"additional","affiliation":[{"name":"Hainan Institute, Zhejiang University Yazhou Bay Science and Technology City  Sanya China"},{"name":"Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Horticultural Plant Growth and Development Ministry of Agriculture and Rural Affairs  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9248-0712","authenticated-orcid":false,"given":"Mingfang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hainan Institute, Zhejiang University Yazhou Bay Science and Technology City  Sanya China"},{"name":"Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Horticultural Plant Growth and Development Ministry of Agriculture and Rural Affairs  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0686-8882","authenticated-orcid":false,"given":"Jinghua","family":"Yang","sequence":"additional","affiliation":[{"name":"Hainan Institute, Zhejiang University Yazhou Bay Science and Technology City  Sanya China"},{"name":"Laboratory of Germplasm Innovation and Molecular Breeding, Institute of Vegetable Science Zhejiang University  Hangzhou China"},{"name":"Key Laboratory of Horticultural Plant Growth and Development Ministry of Agriculture and Rural Affairs  Hangzhou 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H.(1968)Studies on the new male\u2010sterility in Japanese radish with special reference to the utilization of this sterility towerds the practical raising of hybrid seeds. Memoirs of the Faculty of Agriculture Kagoshima University 6."},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.19.00157"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab236"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3122"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2021.01.008"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2021.08.019"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41576-021-00432-x"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00497-019-00371-y"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15715"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-352"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.107.057208"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042811-105552"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-29648-z"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature12030"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhab039"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1270\/jsbbs.64.38"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1673-8527(08)60010-7"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-10-231"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/ggn2.202100019"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1705189114"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00122-020-03635-8"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab315"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13895"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5884"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2003.12.001"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.14268","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,4,17]],"date-time":"2024-04-17T09:53:12Z","timestamp":1713347592000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.14268"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,11]]},"references-count":48,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2024,5]]}},"alternative-id":["10.1111\/pbi.14268"],"URL":"https:\/\/doi.org\/10.1111\/pbi.14268","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,11]]},"assertion":[{"value":"2023-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-11-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-01-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}