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The VQ\u2010WRKY complex plays a crucial role in the response of plants to biotic stresses. However, our understanding of the defensive role of the VQ\u2010WRKY complex on woody plants, particularly apples, under biotic stress, remains limited. In this study, we elucidated the molecular mechanisms underlying the defensive role of the apple MdVQ37\u2010MdWRKY100 module in response to GLS infection. The overexpression of\n                    <jats:italic>MdWRKY100<\/jats:italic>\n                    enhanced resistance to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>fructicola<\/jats:italic>\n                    , whereas MdWRKY100 RNA interference in apple plants reduced resistance to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>fructicola<\/jats:italic>\n                    by affecting salicylic acid (SA) content and the expression level of the CC\u2010NBS\u2010LRR resistance gene\n                    <jats:italic>MdRPM1<\/jats:italic>\n                    . DAP\u2010seq, Y1H, EMSA, and RT\u2010qPCR assays indicated that MdWRKY100 inhibited the expression of\n                    <jats:italic>MdWRKY17<\/jats:italic>\n                    , a positive regulatory factor gene of SA degradation, upregulated the expression of\n                    <jats:italic>MdPAL1<\/jats:italic>\n                    , a key enzyme gene of SA biosynthesis, and promoted\n                    <jats:italic>MdRPM1<\/jats:italic>\n                    expression by directly binding to their promotors. Transient overexpression and silencing experiments showed that MdPAL1 and MdRPM1 positively regulated GLS resistance in apples. Furthermore, the overexpression of\n                    <jats:italic>MdVQ37<\/jats:italic>\n                    increased the susceptibility to\n                    <jats:italic>C<\/jats:italic>\n                    .\n                    <jats:italic>fructicola<\/jats:italic>\n                    by reducing the SA content and expression level of\n                    <jats:italic>MdRPM1<\/jats:italic>\n                    . Additionally, MdVQ37 interacted with MdWRKY100, which repressed the transcriptional activity of MdWRKY100. In summary, these results revealed the molecular mechanism through which the apple MdVQ37\u2010MdWRKY100 module responds to GLS infection by regulating SA content and\n                    <jats:italic>MdRPM1<\/jats:italic>\n                    expression, providing novel insights into the involvement of the VQ\u2010WRKY complex in plant pathogen defence responses.\n                  <\/jats:p>","DOI":"10.1111\/pbi.14351","type":"journal-article","created":{"date-parts":[[2024,4,29]],"date-time":"2024-04-29T12:30:36Z","timestamp":1714393836000},"page":"2364-2376","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["The\n                    <scp>MdVQ37\u2010MdWRKY100<\/scp>\n                    complex regulates salicylic acid content and\n                    <i>MdRPM1<\/i>\n                    expression to modulate resistance to Glomerella leaf spot in apples"],"prefix":"10.1111","volume":"22","author":[{"given":"Qinglong","family":"Dong","sequence":"first","affiliation":[{"name":"College of Forestry Hebei Agricultural University  Baoding China"},{"name":"State Key Laboratory of Crop Stress Biology for Arid Areas\/Shaanxi Key Laboratory of Apple College of Horticulture, Northwest A &amp; 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