{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T06:13:15Z","timestamp":1791612795222,"version":"4.3.4"},"reference-count":69,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T00:00:00Z","timestamp":1717891200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32272655"],"award-info":[{"award-number":["32272655"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Apple is an important cash crop in China, and it is susceptible to fungal infections that have deleterious effects on its yield. Apple bitter rot caused by\n                    <jats:italic>Colletorichum gloeosporioides<\/jats:italic>\n                    is one of the most severe fungal diseases of apple. Salicylic acid (SA) is a key signalling molecule in the plant disease resistance signalling pathways. Lignin synthesis also plays a key role in conferring disease resistance. However, few studies have clarified the relationship between the SA disease resistance signalling pathway and the lignin disease resistance pathway in apple.\n                    <jats:italic>MdMYB46<\/jats:italic>\n                    has previously been shown to promote lignin accumulation in apple and enhance salt and osmotic stress tolerance. Here, we investigated the relationship between\n                    <jats:italic>MdMYB46<\/jats:italic>\n                    and biological stress; we found that\n                    <jats:italic>MdMYB46<\/jats:italic>\n                    overexpression enhances the resistance of apple to\n                    <jats:italic>C. gloeosporioides<\/jats:italic>\n                    . We also identified MdARF1, a transcription factor upstream of\n                    <jats:italic>MdMYB46<\/jats:italic>\n                    , via yeast library screening and determined that\n                    <jats:italic>MdARF1<\/jats:italic>\n                    was regulated by\n                    <jats:italic>miR7125<\/jats:italic>\n                    through psRNATarget prediction. This regulatory relationship was confirmed through LUC and qRT\u2010PCR experiments, demonstrating that\n                    <jats:italic>miR7125<\/jats:italic>\n                    negatively regulates\n                    <jats:italic>MdARF1<\/jats:italic>\n                    . Analysis of the\n                    <jats:italic>miR7125<\/jats:italic>\n                    promoter revealed that\n                    <jats:italic>miR7125<\/jats:italic>\n                    responds to SA signals. The accumulation of SA level will result in the decrease of\n                    <jats:italic>miR7125<\/jats:italic>\n                    expression level. In sum, the results of our study provide novel insights into the molecular mechanisms underlying the resistance of apple to\n                    <jats:italic>C. gloeosporioides<\/jats:italic>\n                    and reveal a new pathway that enhances lignin accumulation in apple in response to SA signals. These findings provide valuable information for future studies aimed at breeding apple for disease resistance.\n                  <\/jats:p>","DOI":"10.1111\/pbi.14401","type":"journal-article","created":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T06:34:04Z","timestamp":1717914844000},"page":"2741-2755","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":39,"title":["The\n                    <i>miR7125\u2010MdARF1<\/i>\n                    module enhances the resistance of apple to\n                    <i>Colletotrichum gloeosporioides<\/i>\n                    by promoting lignin synthesis in response to salicylic acid signalling"],"prefix":"10.1111","volume":"22","author":[{"given":"Xinmei","family":"Liu","sequence":"first","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Dai","sequence":"additional","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajun","family":"Shi","sequence":"additional","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junqin","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"},{"name":"Key Laboratory of Protected Horticulture of Education of Ministry and Liaoning Province\/National &amp; Local Joint Engineering Research Center of Northern Horticultural Facilities Design &amp; Application Technology  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"He","sequence":"additional","affiliation":[{"name":"Shandong Institute of Pomology  Taian Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Plant Protection Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6555-6451","authenticated-orcid":false,"given":"Yue","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Horticulture Shenyang Agricultural University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,9]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.8475"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1026561029533"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.039982"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-009-9458-1"},{"key":"e_1_2_10_6_1","first-page":"7","article-title":"Effect of salicylic acid on rice seedling distemper resistance","volume":"26","author":"Cai X.Z.","year":"1996","journal-title":"Chin. J. Phytopathol."},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar4142"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0434.2007.01254.x"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13151"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pmpp.2007.01.001"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409450102"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2013.09.033"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.127878"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19071883"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2012.10.010"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7050341"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44154-024-00154-0"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2006.02719.x"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2015.00228"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.43.040204.135923"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.2003.01717.x"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0029(20001115)51:4<382::AID-JEMT7>3.0.CO;2-7"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-924025"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.106.048058"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-021-00701-0"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00344-004-0030-6"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.45.062806.094325"},{"key":"e_1_2_10_28_1","first-page":"242","article-title":"Physiological mechanisms of exogenous salicylic acid\u2010induced maize blight resistance","volume":"40","author":"Jiang Y.F.","year":"2009","journal-title":"Guangxi Agric. Sci."},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3965"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-66761-9"},{"key":"e_1_2_10_31_1","first-page":"5","article-title":"Comparison of defence enzyme activities and malondialdehyde content in different apple germplasm induced by Phytophthora infestans","volume":"34","author":"Kong X.H.","year":"2017","journal-title":"J. Qingdao Agric. Univ."},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.10.4076"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.112.199703"},{"key":"e_1_2_10_34_1","first-page":"20817","article-title":"Salicylic acid mediates the reduced growth of lignin down\u2010regulated plants","volume":"108","author":"Lina G.G.","year":"2011","journal-title":"PNAS"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2010.04164.x"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23116323"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI-05-19-0132-R"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7909.2010.00892.x"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.010376"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.139709"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2013.04.004"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.164"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5319-5328.2005"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err031"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2016.01.007"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2008.04.020"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08122"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(03)00058-X"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiab108"},{"key":"e_1_2_10_51_1","first-page":"84","article-title":"Study on salicylic acid\u2010induced postharvest gray mold resistance in apple","volume":"46","author":"Shi Y.L.","year":"2018","journal-title":"J. Northwest A&F Univ."},{"key":"e_1_2_10_52_1","article-title":"MIR390 is involved in regulating anthracnose resistance in apple","volume":"11","author":"Shi J.J.","year":"2022","journal-title":"Plans"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2022.111245"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14078"},{"key":"e_1_2_10_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.1714"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2012.10.058"},{"key":"e_1_2_10_57_1","unstructured":"Tian J.R.(2018)Cloning and Functional Analysis of MdARF3 Gene in Apple. Shenyang Agricultural University."},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.2307\/3870006"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.phyto.050908.135202"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.105.033076"},{"key":"e_1_2_10_61_1","first-page":"50","article-title":"Relationship between resistance of Arabidopsis thaliana to gray mold induced by SA and lignin content","volume":"33","author":"Wang Y.","year":"2007","journal-title":"Plant Conserv."},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants9020272"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scienta.2020.109779"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1038\/35107108"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0023-8333.2005.00298.x"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042811-105449"},{"key":"e_1_2_10_67_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.113.214940"},{"key":"e_1_2_10_68_1","first-page":"199","article-title":"Full\u2010length cloning of banana ARF3 gene and characterisation of its response to Fusarium wilt","volume":"11","author":"Yang H.","year":"2013","journal-title":"Mol. Plant Breed."},{"key":"e_1_2_10_69_1","first-page":"216","article-title":"Induction of disease resistance and phenylpropane metabolism in apricot fruit by preharvest salicylic acid treatment","volume":"36","author":"Zhao Y.T.","year":"2015","journal-title":"Food Sci."},{"key":"e_1_2_10_70_1","first-page":"20","article-title":"Salicylic acid and chitosan induced resistance to spotted leaf drop disease in apple leaves","volume":"39","author":"Zhong X.G.","year":"2013","journal-title":"Plant Prot."}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.14401","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,19]],"date-time":"2024-11-19T06:54:54Z","timestamp":1731999294000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.14401"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,9]]},"references-count":69,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2024,10]]}},"alternative-id":["10.1111\/pbi.14401"],"URL":"https:\/\/doi.org\/10.1111\/pbi.14401","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,6,9]]},"assertion":[{"value":"2023-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-05-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-06-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}