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In this study, the chondriomes of two isonuclear male\u2010fertile and sterile somatic hybrids (SH9A and SH9B, respectively) between the common potato (<jats:italic>S. tuberosum<\/jats:italic> Group <jats:italic>Tuberosum<\/jats:italic>, <jats:italic>tbr<\/jats:italic>) and the wild species <jats:italic>S. commersonii<\/jats:italic> were sequenced and compared to those of parental species to identify mitochondrial genes involved in the expression of male sterility. A putative novel gene (<jats:italic>orf125<\/jats:italic>) was found only in <jats:italic>tbr<\/jats:italic> and in male\u2010sterile hybrids. Physical or functional deletion of <jats:italic>orf125<\/jats:italic> by mtDNA editing in SH9B and its allotopic expression in SH9A clearly demonstrated that <jats:italic>orf125<\/jats:italic> affects male fertility. Besides knockout mutants induced by mitoTALEN and DddA\u2010derived cytosine base editing, specific <jats:italic>orf125<\/jats:italic> missense mutations generated by the latter approach also induced reversion to male fertility in edited SH9B plants, prompting further studies on ORF125 structure\u2013function relationship. The organization of the mitochondrial genome region implicated in CMS was found to be conserved across all common potato accessions, while an identical copy of <jats:italic>tbr orf125<\/jats:italic> was detected in accessions belonging to the <jats:italic>S. berthaultii<\/jats:italic> species complex (<jats:italic>ber<\/jats:italic>). Such findings corroborate the hypothesis that <jats:italic>ber<\/jats:italic> accessions with T\/\u03b2 cytoplasm outcrossed as female with Andean potato, giving rise to the differentiation of the Chilean potato, and highlight the origin of mitochondrial factors contributing to genic\u2010cytoplasmic male sterility in some tuber\u2010bearing <jats:italic>Solanum<\/jats:italic> hybrids. Our results contribute to the development of innovative breeding approaches in potato.<\/jats:p>","DOI":"10.1111\/pbi.70012","type":"journal-article","created":{"date-parts":[[2025,3,23]],"date-time":"2025-03-23T15:41:10Z","timestamp":1742744470000},"page":"1862-1875","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Mitochondrial gene editing and allotopic expression unveil the role of <i>orf125<\/i> in the induction of male fertility in some <i>Solanum<\/i> spp. hybrids and in the evolution of the common potato"],"prefix":"10.1111","volume":"23","author":[{"given":"Rachele","family":"Tamburino","sequence":"first","affiliation":[{"name":"CNR\u2010IBBR Institute of Biosciences and Bioresources  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nunzio","family":"D'Agostino","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences University of Naples Federico II  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gaetano","family":"Aufiero","sequence":"additional","affiliation":[{"name":"Department of Agricultural Sciences University of Naples Federico II  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Nicolia","sequence":"additional","affiliation":[{"name":"CREA Research Centre for Vegetable and Ornamental Crops  Pontecagnano Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo","family":"Facchiano","sequence":"additional","affiliation":[{"name":"CNR\u2010ISA Institute of Food Science  Avellino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah","family":"Giordano","sequence":"additional","affiliation":[{"name":"CNR\u2010ISA Institute of Food Science  Avellino Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorenza","family":"Sannino","sequence":"additional","affiliation":[{"name":"CNR\u2010IBBR Institute of Biosciences and Bioresources  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Paparo","sequence":"additional","affiliation":[{"name":"CNR\u2010IBBR Institute of Biosciences and Bioresources  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9537-1626","authenticated-orcid":false,"given":"Shin\u2010Ichi","family":"Arimura","sequence":"additional","affiliation":[{"name":"Laboratory of Plant Molecular Genetics, Graduate School of Agricultural and Life Sciences The University of Tokyo  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7792-5955","authenticated-orcid":false,"given":"Nunzia","family":"Scotti","sequence":"additional","affiliation":[{"name":"CNR\u2010IBBR Institute of Biosciences and Bioresources  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9418-8849","authenticated-orcid":false,"given":"Teodoro","family":"Cardi","sequence":"additional","affiliation":[{"name":"CNR\u2010IBBR Institute of Biosciences and Bioresources  Portici Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,22]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/23802359.2021.1886016"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0240124"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/23802359.2021.1883486"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3109\/10520296909063335"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-003-0684-8"},{"key":"e_1_2_9_7_1","unstructured":"Andrews S.(2010)FastQC: A Quality Control Tool for High Throughput Sequence Data.https:\/\/www.bioinformatics.babraham.ac.uk\/projects\/fastqc\/"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1134\/S2079059717070024"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15041"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad024"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-94-011-4661-6_24"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_9_13_1","first-page":"1121","article-title":"Breeding diploid F1 hybrid potatoes for propagation from botanical seed (TPS): comparisons with theory and other crops","volume":"11","author":"Bradshaw J.E.","year":"2022","journal-title":"Plan. 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