{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T18:39:10Z","timestamp":1791830350077,"version":"4.3.4"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,14]],"date-time":"2025-03-14T00:00:00Z","timestamp":1741910400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFA0914600"],"award-info":[{"award-number":["2023YFA0914600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFA0909600"],"award-info":[{"award-number":["2021YFA0909600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32070109"],"award-info":[{"award-number":["32070109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M024202\/1"],"award-info":[{"award-number":["BB\/M024202\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/N016807\/1"],"award-info":[{"award-number":["BB\/N016807\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V009729\/1"],"award-info":[{"award-number":["BB\/V009729\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/Y008308\/1"],"award-info":[{"award-number":["BB\/Y008308\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/Y01135X\/1"],"award-info":[{"award-number":["BB\/Y01135X\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000275","name":"Leverhulme Trust","doi-asserted-by":"publisher","award":["RPG\u20102021\u2010286"],"award-info":[{"award-number":["RPG\u20102021\u2010286"]}],"id":[{"id":"10.13039\/501100000275","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000288","name":"Royal Society","doi-asserted-by":"publisher","award":["URF\\R\\180030"],"award-info":[{"award-number":["URF\\R\\180030"]}],"id":[{"id":"10.13039\/501100000288","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515012075,2024A1515012951"],"award-info":[{"award-number":["2025A1515012075,2024A1515012951"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Although Rubisco is the most abundant enzyme globally, it is inefficient for carbon fixation because of its low turnover rate and limited ability to distinguish CO\n                    <jats:sub>2<\/jats:sub>\n                    and O\n                    <jats:sub>2<\/jats:sub>\n                    , especially under high O\n                    <jats:sub>2<\/jats:sub>\n                    conditions. To address these limitations, phytoplankton, including cyanobacteria and algae, have evolved CO\n                    <jats:sub>2<\/jats:sub>\n                    \u2010concentrating mechanisms (CCM) that involve compartmentalizing Rubisco within specific structures, such as carboxysomes in cyanobacteria or pyrenoids in algae. Engineering plant chloroplasts to establish similar structures for compartmentalizing Rubisco has attracted increasing interest for improving photosynthesis and carbon assimilation in crop plants. Here, we present a method to effectively induce the condensation of endogenous Rubisco within tobacco (\n                    <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    ) chloroplasts by genetically fusing superfolder green fluorescent protein (sfGFP) to the tobacco Rubisco large subunit (RbcL). By leveraging the intrinsic oligomerization feature of sfGFP, we successfully created pyrenoid\u2010like Rubisco condensates that display dynamic, liquid\u2010like properties within chloroplasts without affecting Rubisco assembly and catalytic function. The transgenic tobacco plants demonstrated comparable autotrophic growth rates and full life cycles in ambient air relative to the wild\u2010type plants. Our study offers a promising strategy for modulating endogenous Rubisco assembly and spatial organization in plant chloroplasts via phase separation, which provides the foundation for generating synthetic organelle\u2010like structures for carbon fixation, such as carboxysomes and pyrenoids, to optimize photosynthetic efficiency.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70047","type":"journal-article","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T00:31:16Z","timestamp":1741998676000},"page":"2140-2149","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Engineering Rubisco condensation in chloroplasts to manipulate plant photosynthesis"],"prefix":"10.1111","volume":"23","author":[{"given":"Taiyu","family":"Chen","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Utilization and Conservation of Food and Medicinal Resources in Northern Region Shaoguan University  Shaoguan China"},{"name":"Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"},{"name":"National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Hojka","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Sir Ernst Chain Building\u2010Wolfson Laboratories, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philip","family":"Davey","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Essex  Colchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaqi","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tracy","family":"Lawson","sequence":"additional","affiliation":[{"name":"School of Life Sciences, University of Essex  Colchester UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter J.","family":"Nixon","sequence":"additional","affiliation":[{"name":"Department of Life Sciences Sir Ernst Chain Building\u2010Wolfson Laboratories, Imperial College London  London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-4816","authenticated-orcid":false,"given":"Yongjun","family":"Lin","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University  Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8884-4819","authenticated-orcid":false,"given":"Lu\u2010Ning","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Systems, Molecular and Integrative Biology University of Liverpool  Liverpool UK"},{"name":"MOE Key Laboratory of Evolution and Marine Biodiversity &amp; College of Marine Life Sciences Ocean University of China  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,14]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aap9221"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2008.01.001"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14414"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erz275"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-20132-0"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-1679-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/46.special_issue.1269"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02131"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-043015-111633"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1071\/9780643103405"},{"key":"e_1_2_9_13_1","first-page":"1141","article-title":"Advances in understanding carboxysome assembly in Prochlorococcus and Synechococcus implicate CsoS2 as a critical component","volume":"5","author":"Cai F.","year":"2015","journal-title":"Life (Basel)"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-60761-925-3_26"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13497"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37490-0"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koac348"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koae132"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00386231"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.281.5374.237"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.9b00237"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.008"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-020-00811-y"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-081519-040100"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2007990117"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1904587116"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2015.07.008"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0110591"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13776"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15196"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2021.10.004"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.03.019"},{"issue":"133","key":"e_1_2_9_33_1","article-title":"A Spatial Interactome Reveals the Protein Organization of the Algal CO2\u2010Concentrating Mechanism","volume":"171","author":"Mackinder L.C.M.","year":"2017","journal-title":"Cell"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx178"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2010.00575.x"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32004-w"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-41211-y"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-4669.2007.00127.x"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-020-0387-7"},{"key":"e_1_2_9_40_1","first-page":"707","article-title":"Surveying rubisco diversity and temperature response to improve crop photosynthetic efficiency","volume":"172","author":"Orr D.J.","year":"2016","journal-title":"Plant Physiol."},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1424031112"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1172"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erx133"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-18045-y"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.14351"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(75)80083-0"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.53.100301.135233"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.16.00107"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.18.00787"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae665"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.21.8526"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0600605103"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-019-0880-5"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.110.164814"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-07624-w"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41594-021-00676-5"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2007.03261.x"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-042809-112206"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70047","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T08:50:39Z","timestamp":1748508639000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70047"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,14]]},"references-count":57,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,6]]}},"alternative-id":["10.1111\/pbi.70047"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70047","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2024.09.16.613299","asserted-by":"object"}]},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3,14]]},"assertion":[{"value":"2024-09-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-27","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}