{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:33:19Z","timestamp":1791361999735,"version":"4.3.1"},"reference-count":66,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T00:00:00Z","timestamp":1748217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy, Government of India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2025,8]]},"abstract":"<jats:title>Summary<\/jats:title><jats:p>Constitutive expression of hypersensitive cell\u2010death\u2010inducing effectors secreted by pathogens has proved to be difficult in plants. Herein, xylem\u2010specific promoters (<jats:italic>P<\/jats:italic><jats:sub><jats:italic>VND7<\/jats:italic><\/jats:sub> or <jats:italic>P<\/jats:italic><jats:sub><jats:italic>VND2<\/jats:italic><\/jats:sub>) were utilized to express three cell\u2010death\u2010inducing proteins (<jats:italic>FocSIX1A<\/jats:italic>, <jats:italic>FocSIX6<\/jats:italic> or <jats:italic>TvSm1<\/jats:italic>) of fungal origin in banana plants. The regenerated plants were not only healthy but also showed improved resistance to fungal (Foc vcg0124 and Foc vcg0125) infection. These banana plants developed systemic resistance\u2010like symptoms, including elevated jasmonic acid content, callose depositions, increased xylem wall thickness and expression of several defence\u2010associated genes. Interestingly, expression of NAC105, a homologue of VND (vascular\u2010related NAC domain) transcription factor, was elevated in all these transgenic lines. Employing a novel positive\u2010feedback loop using VND genes, banana lines with thicker xylem walls devoid of any ectopic lignification were developed. Enhanced disease resistance of these lines against Foc strains, increased expression of defence genes, elevated jasmonic acid content and quantitative elevation of phenolic compounds provide evidence that elevation in xylem wall thickness does indeed contribute to systemic resistance by playing a crucial role in regulating immune responses. The current study demonstrates that (a) plants that express cell\u2010death\u2010inducing proteins in xylem can be regenerated, (b) these plants are healthy and show enhanced disease resistance and (c) a novel feedback loop involving vascular proteins, which specifically elevates xylem wall thickness without negatively affecting growth, exists in plants.<\/jats:p>","DOI":"10.1111\/pbi.70131","type":"journal-article","created":{"date-parts":[[2025,5,26]],"date-time":"2025-05-26T03:19:02Z","timestamp":1748229542000},"page":"3283-3299","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A novel approach to enhance resistance to vascular disease by expressing cell\u2010death\u2010inducing fungal elicitors in the xylem tissue"],"prefix":"10.1111","volume":"23","author":[{"given":"Sanjana","family":"Negi","sequence":"first","affiliation":[{"name":"National Agri\u2010Food Biotechnology Institute Department of Biotechnology  Mohali India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6423-8809","authenticated-orcid":false,"given":"Himanshu","family":"Tak","sequence":"additional","affiliation":[{"name":"Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Subham","family":"Bhakta","sequence":"additional","affiliation":[{"name":"Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahesh","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Environmental Monitoring and Assessment Division Bhabha Atomic Research Centre  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7678-1291","authenticated-orcid":false,"given":"Thumballi Ramabhatta","family":"Ganapathi","sequence":"additional","affiliation":[{"name":"Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9210-4605","authenticated-orcid":false,"given":"Sudhir","family":"Singh","sequence":"additional","affiliation":[{"name":"Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8776-3021","authenticated-orcid":false,"given":"Anand","family":"Ballal","sequence":"additional","affiliation":[{"name":"Nuclear Agriculture and Biotechnology Division Bhabha Atomic Research Centre  Mumbai India"},{"name":"Homi Bhabha National Institute  Mumbai India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,26]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13807"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/mpp.12821"},{"key":"e_1_2_10_4_1","volume-title":"Global Evaluation of Musa Germplasm for Resistance to Fusarium Wilt, Mycosphaerella Leaf\u00a0Spot\u00a0Diseases and Nematodes. 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