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Hydrogen sulphide (H\n                    <jats:sub>2<\/jats:sub>\n                    S) serves as an important gaseous signalling molecule in plants, mediating a myriad of physiological processes like salt tolerance. However, the molecular mechanisms underlying H\n                    <jats:sub>2<\/jats:sub>\n                    S accumulation and its regulation under salinity stress in alfalfa are still not fully elucidated. In this study, we demonstrated that MsNAC2a, a NAC transcription factor, is a negative modulator of salt stress resistance in alfalfa. Constitutive overexpression of\n                    <jats:italic>MsNAC2a<\/jats:italic>\n                    downregulated the expression of H\n                    <jats:sub>2<\/jats:sub>\n                    S biosynthesis\u2010related genes, such as\n                    <jats:sub>\n                      <jats:italic>L<\/jats:italic>\n                    <\/jats:sub>\n                    <jats:italic>\u2010CYSTEINE DESULFHYDRASE1<\/jats:italic>\n                    (\n                    <jats:italic>MsLCD1<\/jats:italic>\n                    ), and upregulated the\n                    <jats:italic>O\u2010ACETYLSERINE(THIOL)LYASE ISOFORM A1<\/jats:italic>\n                    (\n                    <jats:italic>MsOASA1<\/jats:italic>\n                    ) gene, a key gene involved in H\n                    <jats:sub>2<\/jats:sub>\n                    S metabolism, while also suppressing the expression of reactive oxygen species (ROS) scavenging genes like\n                    <jats:italic>MsCOX11<\/jats:italic>\n                    , leading to a reduction in hydrogen sulphide levels and an increase in ROS accumulation, ultimately impairing the plant's salt tolerance. Furthermore, the AP2\/EREBP\u2010type transcription factor MsEREBP1 physically interacts with MsNAC2a both in\u00a0vivo and in\u00a0vitro, influencing its transcriptional activity and modulating salt stress responses in alfalfa. Conversely, silencing\n                    <jats:italic>MsNAC2a<\/jats:italic>\n                    enhanced salt stress resistance without affecting plant growth or yield. Collectively, our study highlights that MsNAC2a precisely regulates the homeostasis of salt stress responses and provides new insights into the mechanisms by which the cooperative interaction between MsNAC2a and MsEREBP1 fine\u2010tunes the homeostasis of endogenous H\n                    <jats:sub>2<\/jats:sub>\n                    S levels, thereby influencing alfalfa's salt tolerance and offering valuable strategies for improving crop resilience under saline stress.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70307","type":"journal-article","created":{"date-parts":[[2025,8,10]],"date-time":"2025-08-10T12:00:20Z","timestamp":1754827220000},"page":"5297-5320","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Alfalfa Transcription Factor\n                    <scp>MsNAC2a<\/scp>\n                    Orchestrates the Homeostasis of Salt Stress Responses via Reactive Oxygen Species Accumulation and Hydrogen Sulphide Depletion"],"prefix":"10.1111","volume":"23","author":[{"given":"Songchong","family":"Lu","sequence":"first","affiliation":[{"name":"College of Grassland Science Qingdao Agricultural University  Qingdao China"},{"name":"Shandong Key Laboratory for Germplasm Innovation of Saline\u2010Alkaline Tolerant Grasses and Trees Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Grassland Science Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaozi","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Grassland Science Qingdao Agricultural University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanting","family":"Yu","sequence":"additional","affiliation":[{"name":"College of Grassland Science Qingdao Agricultural University  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