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Control strategies mainly rely on chemical fungicides, but resistance development limits their long\u2010term effectiveness. This study introduces, for the first time in crop protection, the use of DNA aptamers as a novel and sustainable strategy. Aptamers are short, single\u2010stranded DNA molecules that bind specific targets with high affinity, acting like \u2018chemical antibodies\u2019. Using SELEX technology, two aptamers, SOD9.14F and SOD9.26F, were designed to target BcSOD1, a superoxide dismutase enzyme essential for fungal virulence and ROS detoxification. Molecular modelling predicted that both aptamers bind within BcSOD1's catalytic pocket. Both aptamers inhibited BcSOD1 enzymatic activity (97.5%) and reduced germination (67%), fungal biomass (58%) and lesion formation (42%) in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B.\u2009cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    \u2010infected tomato leaves (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) and apple fruits (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Malus domestica<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). Fluorescence microscopy confirmed aptamer binding to conidia surfaces. No antifungal effect was observed in the\n                    <jats:italic>\u0394Bcsod1<\/jats:italic>\n                    mutant or with the non\u2010structured control aptamer Ap.AGA, supporting target specificity. RNA\u2010Seq analysis revealed that SOD9.26F interfered with fungal oxidative stress responses and metabolism. Additionally, aptamer application primed tomato plants, activating defence\u2010related gene expression. Interestingly, Ap.AGA aptamer triggered partial priming, suggesting a broader DNA\u2010induced effect. These findings validate BcSOD1 as an antifungal target and highlight aptamers as dual\u2010action agents: impairing fungal development and enhancing plant immunity. This study positions DNA aptamers as specific, effective and sustainable tools for integrated management of grey mould in agriculture.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70317","type":"journal-article","created":{"date-parts":[[2025,8,29]],"date-time":"2025-08-29T10:40:37Z","timestamp":1756464037000},"page":"5778-5796","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["DNA Aptamers Targeting BcSOD1: A Novel Strategy for Controlling\n                    <i>Botrytis cinerea<\/i>\n                    in Sustainable Agriculture"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-1158-8885","authenticated-orcid":false,"given":"Alba","family":"L\u00f3pez\u2010Laguna","sequence":"first","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Universidad de M\u00e1laga  M\u00e1laga Spain"},{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 Universidad de M\u00e1laga, Consejo Superior de Investigaciones Cient\u00edficas (IHSM\u2212UMA\u2212CSIC)  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1lvaro","family":"Polonio","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Universidad de M\u00e1laga  M\u00e1laga Spain"},{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 Universidad de M\u00e1laga, Consejo Superior de Investigaciones Cient\u00edficas (IHSM\u2212UMA\u2212CSIC)  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc\u00eda","family":"Jim\u00e9nez\u2010Castro","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Universidad de M\u00e1laga  M\u00e1laga Spain"},{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 Universidad de M\u00e1laga, Consejo Superior de Investigaciones Cient\u00edficas (IHSM\u2212UMA\u2212CSIC)  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yandira","family":"Morales","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Universidad de M\u00e1laga  M\u00e1laga Spain"},{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 Universidad de M\u00e1laga, Consejo Superior de Investigaciones Cient\u00edficas (IHSM\u2212UMA\u2212CSIC)  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1412-4031","authenticated-orcid":false,"given":"Alejandra","family":"Vielba\u2010Fern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda, Facultad de Ciencias Universidad de M\u00e1laga  M\u00e1laga Spain"},{"name":"Instituto de Hortofruticultura Subtropical y Mediterr\u00e1nea \u2018La Mayora\u2019 Universidad de M\u00e1laga, Consejo Superior de Investigaciones Cient\u00edficas (IHSM\u2212UMA\u2212CSIC)  M\u00e1laga Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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