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Here, we characterise the cysteine\u2010rich secretory protein\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP from the broad\u2010host\u2010range fungal pathogen\n                    <jats:italic>Sclerotinia sclerotiorum<\/jats:italic>\n                    as a potent elicitor of immune responses in tomato (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). Using an optimised\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                    <\/jats:styled-content>\n                    expression system, we successfully produced bioactive\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP and demonstrated its ability to induce hallmark immune responses, including reactive oxygen species (ROS) bursts and extensive transcriptional reprogramming. Time\u2010course transcriptomic analysis after\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP treatment revealed the rapid activation of defence pathways coupled with suppression of photosynthetic processes. While tomato lacks orthologs of the\n                    <jats:italic>Arabidopsis Ss<\/jats:italic>\n                    SCP receptor\n                    <jats:italic>At<\/jats:italic>\n                    RLP30, we identified a candidate receptor that may mediate\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP recognition in tomato. SsSCP activates immune responses independent of the EDS1\u2013PAD4 module in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    . Remarkably, transgenic expression of\n                    <jats:italic>AtRLP30<\/jats:italic>\n                    in tomato conferred resistance against both bacterial (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas syringae<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) and fungal (\n                    <jats:italic>S. sclerotiorum<\/jats:italic>\n                    and\n                    <jats:italic>Botrytis cinerea<\/jats:italic>\n                    ) pathogens without compromising plant growth. Exogenous\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP application similarly enhanced disease resistance, with protection levels matching those of\n                    <jats:italic>AtRLP30<\/jats:italic>\n                    \u2010expressing lines. Our findings reveal both conserved and species\u2010specific aspects of\n                    <jats:italic>Ss<\/jats:italic>\n                    SCP recognition while establishing its potential as a sustainable crop protection strategy. This research advances our understanding of fungal pattern recognition in crops and provides new tools for plant immunity research and disease control.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70328","type":"journal-article","created":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T08:42:54Z","timestamp":1757666574000},"page":"472-485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A Fungal Cysteine\u2010Rich Protein\n                    <i>Ss<\/i>\n                    SCP Activates Immune Responses and Confers Broad\u2010Spectrum Disease Resistance in Tomato"],"prefix":"10.1111","volume":"24","author":[{"given":"Liyuan","family":"Zhang","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Collaborative Innovation Center of Agri\u2010Seeds, Joint Center for Single Cell Biology, School of Agriculture and Biology Shanghai Jiao Tong University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuhang","family":"Liu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinwen","family":"Yang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaihao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengzhang","family":"Du","sequence":"additional","affiliation":[{"name":"Key Laboratory of Evaluation and Utilization for Special Crops Germplasm Resource in the Southwest Mountains Ministry of Agriculture and Rural Affairs (Co\u2010Construction by the Ministry and Province)  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiajing","family":"Xiao","sequence":"additional","affiliation":[{"name":"Center for Agroforestry Mega Data Science, Haixia Institute of Science and Technology Fujian Agriculture and Forestry University  Fuzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ling","family":"Qing","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8687-2528","authenticated-orcid":false,"given":"Xinhua","family":"Sun","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Plant Disease Biology, College of Plant Protection Southwest University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"776","article-title":"Gene Set Enrichment Analysis With topGO","volume":"27","author":"Alexa A.","year":"2009","journal-title":"Bioconductor Improv"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu638"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-021-00874-5"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1364-3703.2005.00316.x"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-phyto-080614-120106"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2307604120"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_9_9_1","first-page":"169","volume-title":"Methods in Enzymology","author":"Cregg J. 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