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However, the biosynthesis of scoparone, the major active component in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. capillaris<\/jats:italic>\n                    <\/jats:styled-content>\n                    , remains unclear. Here, a full\u2010length transcriptomic and integrated metabolomic dataset of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. capillaris<\/jats:italic>\n                    <\/jats:styled-content>\n                    was generated for the first time. A nearly complete map of the biosynthesis pathway of scoparone was subsequently proposed. Notably, a novel\n                    <jats:italic>O<\/jats:italic>\n                    \u2010methyltransferase,\n                    <jats:italic>AcOMT1<\/jats:italic>\n                    , which catalyses the methylation of scopoletin and isoscopoletin as the final step in the biosynthesis of scoparone, was annotated and characterised using a weighted gene correlation network, phylogenetic analysis, subcellular localisation analysis and enzyme assays. Molecular docking and dynamics simulations demonstrated that AcOMT1 has a stronger and more stable affinity for scopoletin than for isoscopoletin, explaining its greater efficiency and preference for the methylation of scopoletin. Ultimately, by coexpressing\n                    <jats:italic>AcOMT1<\/jats:italic>\n                    , along with the crucial genes responsible for scopoletin biosynthesis in\n                    <jats:italic>Nicotiana benthamiana,<\/jats:italic>\n                    we developed a system for scoparone biosynthesis, yielding 3.03\u2009\u03bcg.g\n                    <jats:sup>\u22121<\/jats:sup>\n                    dry weight. This study provides not only a comprehensive understanding of the biosynthetic pathway of scoparone but also new avenues for scoparone production in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. capillaris<\/jats:italic>\n                    <\/jats:styled-content>\n                    via synthetic biology and metabolic engineering.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70398","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T01:39:54Z","timestamp":1760405994000},"page":"346-360","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A Comprehensive Multiomics Approach Illuminates the Biosynthetic Mechanism of Scoparone in\n                    <i>Artemisia capillaris<\/i>"],"prefix":"10.1111","volume":"24","author":[{"given":"Fuyou","family":"Guo","sequence":"first","affiliation":[{"name":"College of Plant 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