{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:35:47Z","timestamp":1791408947809,"version":"4.3.3"},"reference-count":67,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32161143004"],"award-info":[{"award-number":["32161143004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32270586"],"award-info":[{"award-number":["32270586"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013088","name":"Qinglan Project of Jiangsu Province of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100013088","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    As the staple food of over half the world's population, rice with a high resistant starch (RS) content may be beneficial in the dietary prevention of diabetes and hyperlipidemia. Mutating key enzymes in starch metabolism to increase the RS content has been successful in other crops. However, there are always adverse effects, such as low grain weight, wrinkled seeds, and powdery endosperm that accompany the high RS content in rice. Here, we identified two cis\u2010regulatory elements (CREs) related to\n                    <jats:italic>SBEIIb<\/jats:italic>\n                    by combining assay\u2010for\u2010transposase\u2010accessible\u2010chromatin sequencing, DNAase I hypersensitive site sequencing and bioinformatics analysis. The RS content of cre mutants increased by ~5.31\u2010fold compared to the wild type, but the starch morphology, grain shape, and major agronomic traits of the cre mutants were not affected. Analysis of the starch structure revealed that the increase in medium\u2010 and long\u2010chain amylopectin contributed to the high RS phenotype of the cre mutants. Broad transcriptomics and metabolomics showed some changes in sugars, lipids, and amino acids in the mutant endosperm, suggesting that regulation of the starch metabolism pathway triggers the distribution of other metabolic pathways, but with a relatively small difference. The genetic study revealed that transcription factors\n                    <jats:italic>NAC20<\/jats:italic>\n                    ,\n                    <jats:italic>NAC23<\/jats:italic>\n                    and\n                    <jats:italic>NAC26<\/jats:italic>\n                    bind to the CRE1 and CRE2 sequences, co\u2010regulating\n                    <jats:italic>SBEIIb<\/jats:italic>\n                    gene expression. These results provide support for mildly developing high\u2010RS varieties of rice, and possibly of other cereals.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70436","type":"journal-article","created":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T18:26:58Z","timestamp":1761762418000},"page":"1635-1648","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Moderate Regulation of\n                    <i>SBEIIb<\/i>\n                    Gene Expression by Editing Cis\u2010Regulatory Elements Enhances the Resistant Starch Content in Rice"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1811-3051","authenticated-orcid":false,"given":"Qingqing","family":"Yang","sequence":"first","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"},{"name":"Yangzhou Modern Seed Innovation Institute  Gaoyou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Ye","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiannan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Tan","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongsheng","family":"Zhao","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"},{"name":"Yangzhou Modern Seed Innovation Institute  Gaoyou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5543-5798","authenticated-orcid":false,"given":"Qiaoquan","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding\/Zhongshan Biological Breeding Laboratory\/Key Laboratory of Plant Functional Genomics of the Ministry of Education, College of Agriculture Yangzhou University  Yangzhou China"},{"name":"Jiangsu Co\u2010Innovation Center for Modern Production Technology of Grain Crops\/Jiangsu Key Laboratory of Crop Genetics and Physiology Yangzhou University  Yangzhou China"},{"name":"Yangzhou Modern Seed Innovation Institute  Gaoyou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,29]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eru310"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2014860117"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2016.10.007"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-016-1057-7"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(78)91119-1"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313X.1995.07010003.x"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/err188"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-12-223"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.48130\/SeedBio-2023-0004"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003647"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/125.3.459"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr064"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.0c02995"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.135293"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14053"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw982"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2223"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.1923"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw324"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70136"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13519"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2022.01.016"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1146281"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erp297"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-82865-2"},{"key":"e_1_2_8_28_1","first-page":"259","article-title":"A Highly Efficient Transformation System Mediated by Agrobacterium tumefaciens in Rice (Oryza sativa L.)","volume":"24","author":"Liu Q. Q.","year":"1998","journal-title":"Acta Photophysiologica Sinica"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.48130\/SeedBio-2023-0016"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2018.04.004"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-070122-030236"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.01638"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koab093"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-020-00441-0"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf091"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.010127"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/eri292"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1616053113"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jand.2019.10.019"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku365"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510737103"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.030"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.5458\/jag.50.225"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2022.100329"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2021.102136"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2017.00298"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1270\/jsbbs.17069"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2220622120"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.20.00984"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.17.01013"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-48760-w"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.06.004"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-020-02146-5"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac043"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13478"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw209"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043026"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2021.118742"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1093\/nsr\/nwab002"},{"key":"e_1_2_8_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.13798"},{"key":"e_1_2_8_61_1","doi-asserted-by":"publisher","DOI":"10.48130\/SeedBio-2023-0005"},{"key":"e_1_2_8_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.6b05448"},{"key":"e_1_2_8_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13010"},{"key":"e_1_2_8_64_1","doi-asserted-by":"publisher","DOI":"10.1002\/jsfa.5987"},{"key":"e_1_2_8_65_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkv962"},{"key":"e_1_2_8_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14422"},{"key":"e_1_2_8_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1615104113"},{"key":"e_1_2_8_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.carbpol.2023.121141"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70436","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70436","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70436","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T20:49:08Z","timestamp":1773002948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70436"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,29]]},"references-count":67,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/pbi.70436"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70436","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,10,29]]},"assertion":[{"value":"2025-07-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-15","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}