{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T01:03:40Z","timestamp":1782781420760,"version":"3.54.5"},"reference-count":34,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2021YFD1700100"],"award-info":[{"award-number":["2021YFD1700100"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["22477064"],"award-info":[{"award-number":["22477064"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The development of dual\u2010target inhibitors represents a cost\u2010effective strategy for integrated pest management. Here, we report the first dual\u2010target inhibitors designed against the evolutionarily conserved domain of phytoene synthase (PSY), a key enzyme in carotenoid biosynthesis. Using comparative genomics, we identified structural conservation between PSY in plants and squalene synthase (erg9) in fungi. Through virtual screening and structure\u2010based optimization of compounds targeting PSY, we identified lead compound\n                    <jats:bold>1c<\/jats:bold>\n                    , which exhibited potent herbicidal and fungicidal activity. In\u00a0vitro binding assays confirmed that\n                    <jats:bold>1c<\/jats:bold>\n                    binds to both PSY and erg9. In plants,\n                    <jats:bold>1c<\/jats:bold>\n                    treatment reduced chlorophyll content, downregulated photosynthesis\u2010associated genes, and caused substrate accumulation in the carotenoid pathway. In fungi,\n                    <jats:bold>1c<\/jats:bold>\n                    induced a mycelial morphology identical to erg9 knockout mutants. Molecular dynamics simulations revealed the differential binding conformations of\n                    <jats:bold>1c<\/jats:bold>\n                    to PSY and erg9, elucidating its mode of action. This work establishes PSY and its homologues as a promising target for the development of novel, broad\u2010spectrum dual\u2010action agrochemicals based on targetome structural similarity.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70531","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T12:15:56Z","timestamp":1767960956000},"page":"2903-2913","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A Novel Dual\u2010Target Compound Designed With Potent Herbicidal and Fungicidal Activity Inspired by Conserved Phytoene Synthase Domains"],"prefix":"10.1111","volume":"24","author":[{"given":"Di","family":"Zhang","sequence":"first","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Li","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunxue","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4000-0745","authenticated-orcid":false,"given":"Nuo","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhilei","family":"Yu","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tianyi","family":"Shen","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baifan","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8796-4264","authenticated-orcid":false,"given":"Qiang","family":"Bian","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dejun","family":"Ma","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yucheng","family":"Gu","sequence":"additional","affiliation":[{"name":"Syngenta Jealott's Hill International Research Centre  Bracknell UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han","family":"Xu","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Xi","sequence":"additional","affiliation":[{"name":"National Pesticide Engineering Research Center (Tianjin), Department of Chemical Biology, State Key Laboratory of Element\u2010Organic Chemistry, College of Chemistry Nankai University  Tianjin P. R. China"},{"name":"Frontiers Science Center for New Organic Matter Nankai University  Tianjin P. R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1526-4998(200003)56:3<245::AID-PS133>3.0.CO;2-A"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.02.003"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.5087"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b05051"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01616-07"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3598"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.3696"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.4760"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1369-5266(00)00163-1"},{"key":"e_1_2_8_12_1","volume-title":"Plant Toxicology","author":"Hock B.","year":"2005"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-024-02437-w"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fgb.2013.02.001"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42256-025-01091-x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2014.12.007"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ps.6285"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koae030"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plipres.2018.04.004"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2009.03966.x"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1199\/tab.0158"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01454-06"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.0c04252"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf0006426"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1614\/WT-D-16-00046.1"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90462-C"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2017.09.010"},{"key":"e_1_2_8_28_1","unstructured":"United Nations DoEaSA Population Division.2017.\u201cWorld Population Prospects: The 2017 Revision Key Findings and Advance Tables. Working Paper No. ESA\/P\/WP\/248. UN.\u201d"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06415-8"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/0048-3575(87)90134-9"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.18.01122"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.134317"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.66.1671"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2025.01.046"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.884720"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70531","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70531","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70531","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:04:37Z","timestamp":1777608277000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,9]]},"references-count":34,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/pbi.70531"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70531","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,9]]},"assertion":[{"value":"2025-09-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-09","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}