{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T21:46:37Z","timestamp":1789422397065,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U22A20446"],"award-info":[{"award-number":["U22A20446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32322007"],"award-info":[{"award-number":["32322007"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32100192"],"award-info":[{"award-number":["32100192"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The synthesis and accumulation of active ingredients in medicinal plants are distributed in specific organs, tissues, and cell types, which are important for the exploitation of medicinal plants. However, the fine distribution of active ingredients is difficult to know. Here, the system of mass spectrometry imaging (MSI) integrated with single\u2010cell RNA sequencing was established for the first time in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Andrographis paniculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. paniculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), a medicinal plant widely utilised in China and Southeast Asia. MSI shows specific distribution of andrographolides in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. paniculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    , with higher accumulation in non\u2010veinal leaf regions and outer stem cortex (leaf\u2009&gt;\u2009stem; outer\u2009&gt;\u2009inner cortex), as validated by LC\u2010QQQ\u2010MS\/MS assays. Leaf scRNA\u2010seq demonstrates that\n                    <jats:italic>ApCPS2<\/jats:italic>\n                    (the key terpene synthase for andrographolide biosynthesis) exhibits pronounced cell\u2010type\u2010specific expression in photosynthetic mesophyll subclusters, indicating mesophyll cells as the primary site for light\u2010modulated andrographolide production. Interestingly, light may enhance the accumulation of andrographolide biosynthesis, confirming the light sensitivity of metabolism in mesophyll cells. This study explores medicinal components' multidimensional spatial distributions and biosynthetic pathways in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. paniculata<\/jats:italic>\n                    <\/jats:styled-content>\n                    via MSI combined with single\u2010cell technology, providing a novel strategy for determining plant metabolites' fine synthesis and distribution.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70534","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T14:41:44Z","timestamp":1767883304000},"page":"2876-2890","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Mass Spectrometry Imaging Combined With Single\u2010Cell Transcriptional Profiling Reveals the Multidimensional Spatial Distributions and Biosynthetic Pathways of Medicinal Components in\n                    <scp>\n                      <i>Andrographis paniculata<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7673-7776","authenticated-orcid":false,"given":"HaiSheng","family":"Zeng","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"National Immunological Laboratory for Traditional Chinese Medicine Affiliated Hospital of Youjiang Medical University for Nationalities  Baise China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"MeiHui","family":"Shi","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-7761-8203","authenticated-orcid":false,"given":"ZhiRong","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"XueJing","family":"Sun","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HuiJie","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YuCheng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Ren","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"HuiLing","family":"Huang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Almaz","family":"Borjigidai","sequence":"additional","affiliation":[{"name":"Chinese Medicine Guangdong Laboratory Guangdong Hengqin  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-2154-4867","authenticated-orcid":false,"given":"SuJuan","family":"Duan","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Jun","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Lei","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Chinese Medicine Guangdong Laboratory Guangdong Hengqin  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010Bin","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Pharmaceutical Sciences\/State Key Laboratory of Traditional Chinese Medicine Syndrome Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Institute of Medical Plant Physiology and Ecology\/Key Laboratory of Chinese Medicinal Resource From Lingnan, School of Pharmaceutical Sciences Guangzhou University of Chinese Medicine  Guangzhou China"},{"name":"Chinese Medicine Guangdong Laboratory Guangdong Hengqin  Zhuhai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1039\/C5AN01065A"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202401297"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44307-024-00014-y"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/jms.4674"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2102050118"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-021-03177-y"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2024.112046"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lwt.2015.08.062"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2010.08.018"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2023.124721"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13708"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-017-0668-7"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.7145"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113954"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2022.889365"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/jo501529k"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114054"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00393231"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2023.100665"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.14585"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.04.055"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.50.1.47"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-024-01771-3"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2022.112323"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15927"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.indcrop.2025.121715"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.01357"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3390\/jof7060436"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12050403"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2025.05.027"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.orglett.8b03236"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/metabo12010048"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2010.05.022"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41477-022-01291-y"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-physchem-061020-053416"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-arplant-050312-120116"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14572"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.70198"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.202425303"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2020.108472"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2021.100235"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.1051756"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1521959113"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1002\/anie.201813744"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29040852"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xplc.2023.100630"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejmech.2021.113710"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.03.048"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-024-01356-5"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00216-020-03023-7"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.817378"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70534","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:04:47Z","timestamp":1777608287000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,1,8]]},"references-count":51,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1111\/pbi.70534"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70534","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1,8]]},"assertion":[{"value":"2025-06-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-12-26","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}