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Protein\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation, a key post\u2010translational modification, serves as a critical mechanism for nitric oxide (NO) to exert its biological function under adverse conditions. However, the relationship among H\n                    <jats:sub>2<\/jats:sub>\n                    , NO and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation in response to salt stress remains poorly understood. In this study, we demonstrate that NO participated in H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010enhanced salt tolerance in tomato seedlings (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Solanum lycopersicum<\/jats:italic>\n                    <\/jats:styled-content>\n                    cv. Micro\u2010Tom). H\n                    <jats:sub>2<\/jats:sub>\n                    triggered an increase in NO levels and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosothiol (SNO) content under salt stress, enhancing the enrichment of\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylated proteins.\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosoproteomic analysis revealed that MEK1, a conserved MAPK component, was notably induced and\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylated by NO and H\n                    <jats:sub>2<\/jats:sub>\n                    under salt stress. Furthermore,\n                    <jats:italic>SlMEK1<\/jats:italic>\n                    \u2010overexpressing tomato lines exhibited enhanced salt stress tolerance, while knockout lines showed reduced tolerance, indicating the positive regulatory role of\n                    <jats:italic>SlMEK1<\/jats:italic>\n                    in salt tolerance. Additionally,\n                    <jats:italic>SlMEK1<\/jats:italic>\n                    may contribute to H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010enhanced salt tolerance. Meanwhile, MEK1 was shown to undergo\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation at Cys 172, and this modification was involved in H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010enhanced salinity tolerance. Moreover, site\u2010specific mutation analysis at Cys 172 confirmed that MEK1\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation positively contributed to both intrinsic and H\n                    <jats:sub>2<\/jats:sub>\n                    \u2010facilitated salt tolerance. Finally,\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation of MEK1 increased its interaction with GSNOR, and H\n                    <jats:sub>2<\/jats:sub>\n                    further promoted this interaction under salt stress. Collectively, our findings indicate that H\n                    <jats:sub>2<\/jats:sub>\n                    may enhance the salinity tolerance in tomato seedlings by orchestrating the interplay between\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylated MEK1 and GSNOR.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70585","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T09:47:05Z","timestamp":1771408025000},"page":"3656-3678","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hydrogen Gas Enhances Salinity Tolerance in Tomato Seedlings by Regulating the\n                    <i>S<\/i>\n                    \u2010Nitrosylation of MEK1"],"prefix":"10.1111","volume":"24","author":[{"given":"Hua","family":"Fang","sequence":"first","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuetong","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengjing","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinfang","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiya","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuejuan","family":"Pan","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huan","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dandan","family":"Zheng","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caicai","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemei","family":"Hou","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuya","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Aridland Crop Science Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7404-2145","authenticated-orcid":false,"given":"Weibiao","family":"Liao","sequence":"additional","affiliation":[{"name":"College of Horticulture, Gansu Agricultural University  Lanzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert396"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-44081-1_17"},{"key":"e_1_2_9_4_1","unstructured":"Chaki M.2007.\u201cFunction of Reactive Nitrogen Species in Sunflower (Helianthus annuus) in Response to Abiotic and Biotic Stresses. 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