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Here, we demonstrate that the constitutive and inducible expression of banana\u2010derived\n                    <jats:italic>WUS2<\/jats:italic>\n                    (\n                    <jats:italic>GN\u2010WUS2<\/jats:italic>\n                    ) enhances regeneration in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Nicotiana tabacum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (tobacco) and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Musa acuminata<\/jats:italic>\n                    <\/jats:styled-content>\n                    (banana) cv. Grand Naine, even in hormone\u2010free MS medium. Constitutive (CaMV35S::\n                    <jats:italic>GN\u2010WUS2<\/jats:italic>\n                    ) expression promoted shoot formation and modulated hormonal and morphogenic gene expression, as evidenced by molecular, biochemical and histological analyses. However, it caused some pleiotropic effects. To overcome this, glucocorticoid receptor\u2010based inducible\n                    <jats:italic>GN\u2010WUS2<\/jats:italic>\n                    expression enabled healthy shoot development. The upregulated expression of\n                    <jats:italic>HMGR1<\/jats:italic>\n                    ,\n                    <jats:italic>IPPI2<\/jats:italic>\n                    and\n                    <jats:italic>SMT1\u20102<\/jats:italic>\n                    in transgenic tobacco lines boosted isoprenoid and phytosterol biosynthesis and correlated with increased cell division, biomass, pod size and seed yield. Proteomic analysis of seeds from transgenic tobacco lines revealed an enrichment of lipid\u2010associated proteins and the accumulation of the novel lipid adipic acid, supported by expression profiling of\n                    <jats:italic>NtKA<\/jats:italic>\n                    and\n                    <jats:italic>NtSA<\/jats:italic>\n                    genes. Collectively, these results establish\n                    <jats:italic>GN\u2010WUS2<\/jats:italic>\n                    as a master regulator that integrates developmental reprogramming with novel phytosterol biosynthesis and yield enhancement, presenting its versatile role in next\u2010generation regeneration and crop improvement.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70625","type":"journal-article","created":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T13:55:06Z","timestamp":1773323706000},"page":"4174-4189","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Cross\u2010Species Reprogramming of Developmental Plasticity and Metabolic Rewiring via Banana\u2010Derived\n                    <i>WUS2<\/i>\n                    Developmental Regulator"],"prefix":"10.1111","volume":"24","author":[{"given":"Roni","family":"Chaudhary","sequence":"first","affiliation":[{"name":"Plant Tissue Culture and Genetic Engineering Lab BRIC\u2010National Agri\u2010Food and Biomanufacturing Institute (BRIC\u2010NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India)  Mohali Punjab India"},{"name":"Regional Centre for Biotechnology  Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Surender","family":"Singh","sequence":"additional","affiliation":[{"name":"Plant Tissue Culture and Genetic Engineering Lab BRIC\u2010National Agri\u2010Food and Biomanufacturing Institute (BRIC\u2010NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India)  Mohali Punjab India"},{"name":"Regional Centre for Biotechnology  Faridabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Usman","family":"Ali","sequence":"additional","affiliation":[{"name":"Plant Tissue Culture and Genetic Engineering Lab BRIC\u2010National Agri\u2010Food and Biomanufacturing Institute (BRIC\u2010NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India)  Mohali Punjab India"},{"name":"Department of Food Technology Guru Jambheshwar University of Science and Technology  Hisar Haryana India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3958-8465","authenticated-orcid":false,"given":"Siddharth","family":"Tiwari","sequence":"additional","affiliation":[{"name":"Plant Tissue Culture and Genetic Engineering Lab BRIC\u2010National Agri\u2010Food and Biomanufacturing Institute (BRIC\u2010NABI), Department of Biotechnology, Ministry of Science and Technology (Government of India)  Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,12]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/moda.70014"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1467-7652.2009.00458.x"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11240-008-9401-1"},{"key":"e_1_2_10_5_1","first-page":"3401","article-title":"Transgene\u2010Free Genome Editing Supports the Role of Carotenoid Cleavage Dioxygenase 4 as a Negative Regulator of \u03b2\u2010Carotene in Banana","volume":"73","author":"Awasthi P.","year":"2022","journal-title":"Journal of Experimental 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