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Previous research has indicated that CGs can serve as a significant reservoir of organic nitrogen in plants. However, the extent to which its high\u2010CG content contributes to efficient nitrogen utilisation and adaptation to low nitrogen (N) in cassava remains to be further elucidated. This study represents the first identification of MeHNL11 as a bifunctional protein. In response to N deficiency, the hydroxynitrile lyase activity of MeHNL11 promotes the generation and accumulation of cyanide and the Cys245 residue of MeHNL11 is critical for its nuclear oligomerization, in which the protein functions as a transcription factor. Following the cyanide transmission into the nucleus, the oligomeric form of MeHNL11 dissociates into monomers, leading to a dramatic upregulation of\n                    <jats:italic>MeCAS1b<\/jats:italic>\n                    transcription. This regulatory mechanism helps sustain intracellular cyanide homeostasis within cassava and facilitates the synthesis of primary N metabolites, thereby alleviating N deficiency. The exogenous application of the cyanide antidote hydroxocobalamin (COB) inhibited cyanide assimilation by MeCAS1b, leading to exacerbated N deficiency symptoms, such as leaf yellowing and a significant reduction in the contents of NH\n                    <jats:sub>4<\/jats:sub>\n                    <jats:sup>+<\/jats:sup>\n                    and free amino acids (AA) in cassava seedlings under low\u2010N conditions (LN). Our research demonstrates that the MeHNL11\u2010\n                    <jats:italic>MeCAS1b<\/jats:italic>\n                    module plays a pivotal role in CG recycling, offering new insights into the underlying mechanisms governing cassava's exceptional tolerance to low N stress.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70633","type":"journal-article","created":{"date-parts":[[2026,3,10]],"date-time":"2026-03-10T15:38:17Z","timestamp":1773157097000},"page":"4121-4135","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolic Enzyme\n                    <scp>MeHNL11<\/scp>\n                    Regulates\n                    <scp>\n                      <i>MeCAS1b<\/i>\n                    <\/scp>\n                    Transcription for Cyanide Reutilization in Response to\u00a0Nitrate Deficiency in Cassava"],"prefix":"10.1111","volume":"24","author":[{"given":"Weitao","family":"Mai","sequence":"first","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication\/School of Tropical Agriculture and Forestry State Key Laboratory of Tropical Crop Breeding, Hainan University  Sanya Hainan China"},{"name":"Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences  Haikou Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7256-1486","authenticated-orcid":false,"given":"Ruxue","family":"Bao","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication\/School of Tropical Agriculture and Forestry State Key Laboratory of Tropical Crop Breeding, Hainan University  Sanya Hainan China"},{"name":"Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences  Haikou Hainan 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Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinling","family":"Zhao","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication\/School of Tropical Agriculture and Forestry State Key Laboratory of Tropical Crop Breeding, Hainan University  Sanya Hainan China"},{"name":"Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences  Haikou Hainan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-4212-2053","authenticated-orcid":false,"given":"Huaifang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Sanya Institute of Breeding and Multiplication\/School of Tropical Agriculture and Forestry State Key Laboratory of Tropical Crop Breeding, Hainan University  Sanya Hainan China"},{"name":"Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences  Haikou Hainan 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