{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T17:45:25Z","timestamp":1790185525555,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T00:00:00Z","timestamp":1777075200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32560746"],"award-info":[{"award-number":["32560746"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Carnation (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Dianthus caryophyllus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) contains abundant flavonoid glycosides (FGs), which are important natural functional and colour components. However, there are few reports on the modification of UDP\u2010glycosyltransferases (UGTs) in relation to flavonoids in carnation. In this study, we cloned and characterised a flavonoid 3\u2032\n                    <jats:italic>\u2010O\u2010<\/jats:italic>\n                    glucosyltransferase (\n                    <jats:italic>UGT73A93<\/jats:italic>\n                    ) in carnation in\u00a0vitro. Overexpression of\n                    <jats:italic>UGT73A93<\/jats:italic>\n                    in carnation and tobacco increased flavonoid glycoside accumulation, particularly kaempferol glycosides, while decreasing anthocyanin content and lightening flower colour.\n                    <jats:italic>UGT73A93<\/jats:italic>\n                    also enhanced fungal resistance, antioxidant capacity, anti\u2010amylase and anti\u2010pancreatic lipase activities. Yeast one\u2010hybrid and dual\u2010luciferase assays revealed that the\n                    <jats:italic>UGT73A93<\/jats:italic>\n                    promoter interacted with DcH3.3 and DcNAC1, key regulatory proteins involved in flavonoid biosynthesis. We predicted the interaction between DcLON2 and DcNAC1 using AlphaFold 3 and confirmed this hypothesis through yeast two\u2010hybrid assay and bimolecular fluorescence complementation assays. These findings suggest an epigenetic\u2010transcriptional cascade (DcH3.3\u2013DcNAC1\u2013UGT73A93) wherein DcH3.3 opens chromatin for DcNAC1\u2010mediated UGT73A93 activation, while DcLON2 potentially degrades DcNAC1 to form a feedback loop. These results provide new insights into flavonoid 3\u2032\n                    <jats:italic>\u2010O\u2010<\/jats:italic>\n                    glucosyltransferase and may contribute to future strategies aimed at improving the benefits of flavonoid biosynthesis for both plants and humans. It also demonstrates that AI can be applied in the field of plant biosynthesis, accelerating the process of plant breeding.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70674","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T12:51:06Z","timestamp":1777121466000},"page":"4863-4877","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<scp>DcH3.3<\/scp>\n                    and\n                    <scp>DcNAC1<\/scp>\n                    Regulate the Expression of\n                    <scp>\n                      <i>UGT73A93<\/i>\n                    <\/scp>\n                    Involved in the Changes in Flower Colour and Fungal Resistance in Carnation"],"prefix":"10.1111","volume":"24","author":[{"given":"Xuhong","family":"Zhou","sequence":"first","affiliation":[{"name":"Office of Science and Technology Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qirui","family":"Xiong","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumei","family":"Yang","sequence":"additional","affiliation":[{"name":"Flower Research Institute Yunnan Academy of Agricultural Sciences  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyuan","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanxia","family":"Tao","sequence":"additional","affiliation":[{"name":"School of Chinese Materia Medica Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaomi","family":"Yang","sequence":"additional","affiliation":[{"name":"Office of Science and Technology Yunnan University of Chinese Medicine  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytochem.2020.112347"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-012-1162-0"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/nxab269"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00299-011-1006-1"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.113.113407"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40858-024-00641-9"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2010.01.003"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.16969"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.70284"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2010.02.004"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2022.2126427"},{"key":"e_1_2_11_14_1","first-page":"179","article-title":"Determination and Analysis of Anthocyanin Content in Grape","volume":"28","author":"Li X.","year":"2022","journal-title":"Xiandai Shipin"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcad119"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/hr\/uhad049"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s44281-023-00012-5"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2019.01330"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng1637"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.2503\/hortj.UTD-271"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.10.005"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-15-8261-5_6"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.5511\/plantbiotechnology.21.367"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2004.12.010"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jafc.9b02142"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/03235408.2020.1868734"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2012.02.004"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11101-024-10042-0"},{"key":"e_1_2_11_29_1","first-page":"57","volume-title":"Genetic Engineering of Crop Plants for Food and Health Security: Volume 1","author":"Sharma P.","year":"2024"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2014-15-4-r62"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10265-021-01270-y"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2022.111251"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2019.110286"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11627-017-9836-3"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2022.863482"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-023-02867-3"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1360-1385(00)01720-9"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom10020253"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2024.1438967"},{"key":"e_1_2_11_40_1","first-page":"503","article-title":"Determination of Kaempferidetriglycoside in Diauthus by Hplc","volume":"33","author":"Wang M.","year":"2022","journal-title":"Chemical Research"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26136395"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dst053"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2018.07.021"},{"key":"e_1_2_11_44_1","first-page":"227","article-title":"Inhibitory Effect of Extracts From Nymphaea Hybrid Flowers on Pancreatic Lipase","volume":"42","author":"Zhang D.","year":"2017","journal-title":"Food Science and Technology"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2025.106202"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-35509-6"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41438-020-0296-2"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12870-022-03648-z"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70674","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70674","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70674","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T23:57:21Z","timestamp":1784678241000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70674"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,25]]},"references-count":47,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2026,8]]}},"alternative-id":["10.1111\/pbi.70674"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70674","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,25]]},"assertion":[{"value":"2025-09-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}