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However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery of the editing components to new cultivars. Potyviruses (genus\n                    <jats:italic>Potyvirus<\/jats:italic>\n                    ) comprise the largest group of plant RNA viruses. The main limitation of potyviral vectors to express a non\u2010coding RNA consists of potential insertion of stop codons that interrupt the large open reading frame that encompasses most potyviral genome. This is the case with the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptococcus pyogenes<\/jats:italic>\n                    <\/jats:styled-content>\n                    Cas9 sgRNA scaffold, which contains stop codons in all three possible frames. In this work, we first built on a visual reporter system targeting the two homeologs of\n                    <jats:italic>Nicotiana benthamiana Magnesium chelatase subunit I<\/jats:italic>\n                    (\n                    <jats:italic>CHLI<\/jats:italic>\n                    ). Second, we developed a tobacco etch virus (\n                    <jats:italic>Potyvirus nicotianainsculpentis<\/jats:italic>\n                    )\u2010derived vector for VIGE by engineering a modified Cas9 scaffold, free of stop codons, to maintain the potyviral polyprotein reading frame while ensuring effective editing. This vector self\u2010replicates and moves systemically, delivering sgRNAs efficiently throughout the plant. This allowed us to obtain plants exhibiting a white phenotype with their four alleles edited through in\u00a0vitro regeneration from infected leaves, and also to produce edited progeny. We further demonstrated the vector utility in tomato. Given the conserved biological properties within the genus\n                    <jats:italic>Potyvirus<\/jats:italic>\n                    , these findings may be broadly applicable to other potyviruses, expanding the reach of the VIGE technology.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70675","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T13:26:58Z","timestamp":1777901218000},"page":"4933-4947","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A Modified Cas9 Scaffold Allows Extension of the Virus\u2010Induced Gene Editing Technology to the Large\n                    <i>Potyvirus<\/i>\n                    Genus"],"prefix":"10.1111","volume":"24","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-0488","authenticated-orcid":false,"given":"Fernando","family":"Merwaiss","sequence":"first","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2010Universitat Polit\u00e8cnica de Val\u00e8ncia)  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4695-7890","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Aragon\u00e9s","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2010Universitat Polit\u00e8cnica de Val\u00e8ncia)  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-6425-2317","authenticated-orcid":false,"given":"Arcadio","family":"Garc\u00eda","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2010Universitat Polit\u00e8cnica de Val\u00e8ncia)  Valencia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6535-2889","authenticated-orcid":false,"given":"Jos\u00e9\u2010Antonio","family":"Dar\u00f2s","sequence":"additional","affiliation":[{"name":"Instituto de Biolog\u00eda Molecular y Celular de Plantas (Consejo Superior de Investigaciones Cient\u00edficas\u2010Universitat Polit\u00e8cnica de Val\u00e8ncia)  Valencia 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