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Double\u2010stranded RNA (dsRNA)\u2010induced gene silencing is a promising technology for pest control, but efficient delivery remains a major challenge. This work presents a star polycation (SPc)\u2010based nano\u2010delivery platform that enhances dsRNA internalization and enables effective control of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>B.\u2009cinerea<\/jats:italic>\n                    <\/jats:styled-content>\n                    . SPc assembles with dsRNA via electrostatic interactions, hydrogen bonding and Van der Waals forces, forming stable nanoscale complexes. SPc facilitates dsRNA uptake into fungal mycelia by 2.19\u2010fold, primarily via two activated routes: endocytosis and transmembrane transport, which increase vesicle and particle numbers by 3.74\u2010 and 1.87\u2010fold, respectively. Six genes (\n                    <jats:italic>pkc<\/jats:italic>\n                    ,\n                    <jats:italic>ypt10<\/jats:italic>\n                    ,\n                    <jats:italic>pil1<\/jats:italic>\n                    ,\n                    <jats:italic>mfs1<\/jats:italic>\n                    ,\n                    <jats:italic>mfs2<\/jats:italic>\n                    and\n                    <jats:italic>mfs3<\/jats:italic>\n                    ) in these two routes play crucial roles in the delivery process. RNA interference, chemical inhibition and mutation experiments demonstrate that the two routes interact to optimize the cellular uptake of SPc\u2010loaded dsRNA. Finally, a high\u2010efficiency RNA fungicide is developed, comparable to commercial fungicide in protecting cucumber leaves and tomato fruits. This study reveals the synergistic mechanism of nanocarrier\u2010mediated gene delivery and identifies key genes, supporting the design and application of RNA pesticides.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70689","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T06:07:15Z","timestamp":1780121235000},"page":"5152-5171","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Interactional Endocytosis and Transmembrane Transport Promote Cellular Internalization of Nano\u2010Delivered\n                    <scp>RNA<\/scp>\n                    Drugs for Efficient Control of Crop Diseases"],"prefix":"10.1111","volume":"24","author":[{"given":"Mei","family":"Guan","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Xie","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Xue","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinhong","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meizhen","family":"Yin","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuo","family":"Yan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests College of Plant Protection, China Agricultural University  Beijing 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