{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T16:58:12Z","timestamp":1786121892034,"version":"build-2736575974"},"reference-count":71,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T00:00:00Z","timestamp":1786060800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The precise enhancement of nutritional quality in silage maize is a core strategy for increasing livestock production efficiency. Through evolutionary analysis of multiple plant species, we identified two functionally synergistic upstream open reading frames (uORF1 and uORF2) within the 5\u2032 untranslated region of the gene encoding GDP\u2010L\u2010galactose phosphorylase (GGP). By leveraging a natural translation\u2010enhancing haplotype of uORF1 (Hap2) and performing CRISPR\/Cas9\u2010mediated targeted mutagenesis of the highly conserved uORF2, we successfully engineered an elite dual\u2010uORFs variant. This variant significantly increased vitamin C (Vc) content and concurrently improved key silage quality traits, including crude protein and phosphorus levels, without inducing growth penalties. Transcriptomic profiling further elucidated the molecular mechanisms by which the dual\u2010uORFs variation coordinately regulates Vc biosynthesis and the improvement of silage quality. Our findings deepen the understanding of the conventional paradigm of single\u2010uORF regulation and provide a novel strategy and superior germplasm resources for the precision breeding of high\u2010Vc, high\u2010quality silage maize.<\/jats:p>","DOI":"10.1111\/pbi.70731","type":"journal-article","created":{"date-parts":[[2026,8,7]],"date-time":"2026-08-07T07:26:12Z","timestamp":1786087572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Natural and\n                    <scp>CRISPR<\/scp>\n                    \/Cas9 Editing Variations of Dual\u2010\n                    <scp>uORFs<\/scp>\n                    Synergistically Increase the Nutritional Value of Silage Maize Feed"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-7430-9831","authenticated-orcid":false,"given":"Jing","family":"Zhou","sequence":"first","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Wang","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajing","family":"Zhang","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6908-7456","authenticated-orcid":false,"given":"Xinhao","family":"Luo","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuyao","family":"Quan","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Ji","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lixue","family":"Sun","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyang","family":"Chen","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6741-6705","authenticated-orcid":false,"given":"Jin","family":"Chen","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianbo","family":"Cheng","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Fan","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changsheng","family":"Li","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beijiu","family":"Cheng","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Li","sequence":"additional","affiliation":[{"name":"The National Engineering Laboratory of Crop Stress Resistance Breeding Anhui Agricultural University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,7]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"crossref","DOI":"10.1007\/s11250-021-03026-1","article-title":"Stress Amelioration Potential of Vitamin C in Ruminants: A Review","volume":"54","author":"Akinmoladun O. 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