{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T07:45:47Z","timestamp":1785915947912,"version":"3.56.0"},"reference-count":78,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T00:00:00Z","timestamp":1785801600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Sucrose transport and starch accumulation are crucial carbohydrate metabolic processes in rice, playing essential roles during late pollen development to ensure pollen maturation, fertility and high grain production. However, the molecular mechanisms regulating sucrose transport and starch accumulation during rice pollen development remain elusive. In this study, we bred and characterized a semi\u2010fertile rice pollen variant,\n                    <jats:italic>sfp10<\/jats:italic>\n                    , which is derived from highland terraced red rice from Yunnan, China. This variant showed insufficient starch accumulation in pollen, reduced pollen fertility and decreased seed setting compared to the wild type. Using map\u2010based cloning, CRISPR\/Cas9 mutagenesis and gene complementation, the\n                    <jats:italic>OsSUT3<\/jats:italic>\n                    gene, which causes the\n                    <jats:italic>sfp10<\/jats:italic>\n                    variant, was shown to encode a sucrose transporter (SUT) that transports sucrose into pollen for starch synthesis, ensuring pollen viability and fertility. Additionally, transcriptome analysis identified OsWRKY15, a WRKY transcription factor that exhibits a highly similar spatiotemporal expression pattern to\n                    <jats:italic>OsSUT3<\/jats:italic>\n                    in late\u2010developing panicles. Yeast one\u2010hybrid, EMSA, dual\u2010luciferase reporter and ChIP\u2010seq assays demonstrated that OsWRKY15 directly binds to the promoter region of\n                    <jats:italic>OsSUT3<\/jats:italic>\n                    and upregulates its expression. Mutation of\n                    <jats:italic>OsWRKY15<\/jats:italic>\n                    significantly reduced\n                    <jats:italic>OsSUT3<\/jats:italic>\n                    expression and impaired pollen fertility and seed setting and these defects were further exacerbated in the\n                    <jats:italic>wrky15\/OsSUT3\u2010KO<\/jats:italic>\n                    double\u2010knockout lines, while\n                    <jats:italic>OsSUT3<\/jats:italic>\n                    overexpression in the\n                    <jats:italic>wrky15<\/jats:italic>\n                    background significantly rescued pollen fertility and seed setting. These findings demonstrate that the OsWRKY15\u2010OsSUT3 regulatory module is essential for sucrose transport and starch accumulation during late pollen development in rice, providing new insights into the transcriptional regulation of carbon allocation to anthers for rice fertility and grain production.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70738","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:54:12Z","timestamp":1785912852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["The\n                    <scp>OsWRKY15<\/scp>\n                    \u2010\n                    <scp>OsSUT3<\/scp>\n                    Regulatory Module Enhances Rice Pollen Fertility by Mediating Sucrose Allocation to Anthers"],"prefix":"10.1111","author":[{"given":"Qiuping","family":"Li","sequence":"first","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunlong","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Agronomy and Life Sciences, Zhaotong University  Zhaotong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuaibing","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Chemistry and Environmental Engineering Yuxi Normal University  Yuxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Chen","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yandong","family":"Sun","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Ma","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Yong","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qinghui","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lijuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiancheng","family":"Wen","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6924-5186","authenticated-orcid":false,"given":"Dandan","family":"Li","sequence":"additional","affiliation":[{"name":"Rice Research Institute, Yunnan Agricultural University  Kunming China"},{"name":"College of Agronomy and Biotechnology Yunnan Agricultural University  Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,4]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcg030"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/00019606-200603000-00009"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.geoderma.2021.115296"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1537-2197.1979.tb06262.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"1090","DOI":"10.1186\/s12870-024-05808-9","article-title":"Disruption of the OsWRKY71 Transcription Factor Gene Results in Early Rice Seed Germination Under Normal and Cold Stress Conditions","volume":"24","author":"Bataller S.","year":"2024","journal-title":"BMC Plant Biology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2025.102722"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/07352689.2018.1441103"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad181"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12284-019-0333-7"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiac496"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10030239"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.13"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1004384"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2005.02572.x"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2020.1714292"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(75)90682-X"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.196378"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq175"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","DOI":"10.1073\/pnas.2207558119","article-title":"SWEET13 Transport of Sucrose, but Not Gibberellin, Restores Male Fertility in Arabidopsis sweet13; 14","volume":"119","author":"Isoda R.","year":"2022","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiae187"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ery203"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12298-009-0014-x"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2009.244"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10529-012-1100-7"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2024.108522"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.13957"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbi.2010.02.001"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erw324"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232214091"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252111557"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00843-1"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21061909"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes13081323"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms241310640"},{"key":"e_1_2_10_36_1","first-page":"634","article-title":"Physiological Characteristics Analysis and Gene Mapping of a Semi\u2010Sterility Plant Mutant sfp10 in Rice (\n                     Oryza sativa\n                   L.)","volume":"49","author":"Li Q.","year":"2023","journal-title":"Acta Agronomica Sinica"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1093\/plphys\/kiad136"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2021.08.003"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13561"},{"key":"e_1_2_10_40_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.arplant.55.031903.141717"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14224"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2016.04.009"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2013.08.016"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-016-0496-1"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2005.01390.x"},{"key":"e_1_2_10_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2021.04.013"},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00425-005-1530-8"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2022.111297"},{"key":"e_1_2_10_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2010.02.006"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.03.048"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.118992"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-022-07611-x"},{"key":"e_1_2_10_53_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.19.00317"},{"key":"e_1_2_10_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erad028"},{"key":"e_1_2_10_55_1","doi-asserted-by":"crossref","first-page":"koae324","DOI":"10.1093\/plcell\/koae324","article-title":"The Rice microRNA159\u2010SPOROCYTELESS EAR2 Module Regulates Starch Biosynthesis During Pollen Development and Maintains Male Fertility","volume":"37","author":"Tao J.","year":"2024","journal-title":"Plant Cell"},{"key":"e_1_2_10_56_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.14344"},{"key":"e_1_2_10_57_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcr094"},{"key":"e_1_2_10_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.17512"},{"key":"e_1_2_10_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2022.12.012"},{"key":"e_1_2_10_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18368"},{"key":"e_1_2_10_61_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molp.2023.03.008"},{"key":"e_1_2_10_62_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2019.06.005"},{"key":"e_1_2_10_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2229-14-133"},{"key":"e_1_2_10_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2019.153000"},{"key":"e_1_2_10_65_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.14073"},{"key":"e_1_2_10_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/nph.18054"},{"key":"e_1_2_10_67_1","doi-asserted-by":"crossref","DOI":"10.1080\/15592324.2021.1952373","article-title":"Sucrose Transporter in Rice","volume":"16","author":"Wu Y.","year":"2021","journal-title":"Plant Signaling & Behavior"},{"key":"e_1_2_10_68_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11105-013-0633-9"},{"key":"e_1_2_10_69_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.13637"},{"key":"e_1_2_10_70_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgg.2011.08.001"},{"key":"e_1_2_10_71_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.109.073668"},{"key":"e_1_2_10_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/pbi.12527"},{"key":"e_1_2_10_73_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.hpj.2023.09.010"},{"key":"e_1_2_10_74_1","doi-asserted-by":"publisher","DOI":"10.1093\/plcell\/koad223"},{"key":"e_1_2_10_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-9-r137"},{"key":"e_1_2_10_76_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1007880"},{"key":"e_1_2_10_77_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-018-1466-x"},{"key":"e_1_2_10_78_1","doi-asserted-by":"publisher","DOI":"10.1111\/jipb.13271"},{"key":"e_1_2_10_79_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.15921"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70738","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70738","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70738","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T06:54:14Z","timestamp":1785912854000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70738"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,4]]},"references-count":78,"alternative-id":["10.1111\/pbi.70738"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70738","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,4]]},"assertion":[{"value":"2025-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-07-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-04","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pbi.70738"}}