{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:35:07Z","timestamp":1791549307365,"version":"4.3.4"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T00:00:00Z","timestamp":1787529600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172049"],"award-info":[{"award-number":["32172049"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32172387"],"award-info":[{"award-number":["32172387"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010029","name":"Taishan Scholar Foundation of Shandong Province","doi-asserted-by":"publisher","award":["tsqn202211093"],"award-info":[{"award-number":["tsqn202211093"]}],"id":[{"id":"10.13039\/501100010029","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Breeding crops with robust disease resistance often compromises yield, a persistent challenge in agricultural improvement. We report that modifying cell wall architecture through a xylan\u2010specific glycosyltransferase successfully breaks this trade\u2010off in wheat. TaXAX1, a pathogen\u2010induced member of the glycosyltransferase (GT)61 family, catalyses arabinose modification of xylan and is predominantly localized to the Golgi apparatus. CRISPR\/Cas9 knockout mutants of\n                    <jats:italic>Taxax1<\/jats:italic>\n                    exhibited enhanced susceptibility to three major fungal diseases: Fusarium head blight (FHB), Fusarium crown rot (FCR) and stripe rust. Conversely, overexpression of\n                    <jats:italic>TaXAX1<\/jats:italic>\n                    significantly enhanced resistance to all three pathogens concurrently. Remarkably, this multi\u2010disease resistance was achieved without any reduction in grain yield. Mechanistically,\n                    <jats:italic>TaXAX1<\/jats:italic>\n                    mediates cell wall fortification by increasing xylan arabinose modification arabinosylation, upregulating the phenylpropanoid pathway and enhancing the deposition of lignin, cellulose and phenolic cross\u2010linkers. This resulted in a thicker and more reinforced secondary cell wall, which also translated into superior stem strength. Our study identifies\n                    <jats:italic>TaXAX1<\/jats:italic>\n                    as a master regulator of cell wall remodelling and provides a compelling strategy for designing wheat varieties with integrated resilience against multiple diseases and environmental stresses, while safeguarding yield potential.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70747","type":"journal-article","created":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:47:52Z","timestamp":1787564872000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multiplexed Engineering of Disease Resistance and Lodging Tolerance in Wheat Via a Single Xylan Modifying Glycosyltransferase"],"prefix":"10.1111","author":[{"given":"Shufang","family":"Sun","sequence":"first","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhui","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zehua","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyang","family":"Ma","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhui","family":"Niu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3424-8018","authenticated-orcid":false,"given":"Qunqing","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Plant Protection Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7106-2714","authenticated-orcid":false,"given":"Qian","family":"Xu","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Wheat Improvement, College of Agronomy Shandong Agricultural University  Taian China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,24]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"989","DOI":"10.1073\/pnas.1115858109","article-title":"Glycosyl Transferases in Family 61 Mediate Arabinofuranosyl Transfer Onto Xylan in Grasses","volume":"109","author":"Anders N.","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1094\/MPMI-12-19-0341-R","article-title":"Response Regulator 6 (ARR6) Modulates Plant Cell\u2010Wall Composition and Disease Resistance","volume":"33","author":"Bacete L.","year":"2020","journal-title":"Molecular Plant\u2010Microbe Interactions"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","first-page":"343","DOI":"10.1094\/PHYTO.1999.89.4.343","article-title":"Amplified Fragment Length Polymorphism Markers Linked to a Major Quantitative Trait Locus Controlling Scab Resistance in Wheat","volume":"89","author":"Bai G.","year":"1999","journal-title":"Phytopathology"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1007\/s00425-011-1359-2","article-title":"Quantification of Lignin\u2013Carbohydrate Linkages With High\u2010Resolution NMR Spectroscopy","volume":"233","author":"Balakshin M.","year":"2011","journal-title":"Planta"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"17117","DOI":"10.1073\/pnas.1202079109","article-title":"XAX1 From Glycosyltransferase Family 61 Mediates Xylosyltransfer to Rice Xylan","volume":"109","author":"Chiniquy D.","year":"2012","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","first-page":"11850","DOI":"10.1038\/nrm1746","article-title":"Growth of the Plant Cell Wall","volume":"6","author":"Cosgrove D. J.","year":"2005","journal-title":"Nature Reviews. Molecular Cell Biology"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1093\/plcell\/koad325","article-title":"The Plant Cell Wall\u2014Dynamic, Strong, and Adaptable\u2014Is a Natural Shapeshifter","volume":"36","author":"Deborah D.","year":"2024","journal-title":"Plant Cell"},{"key":"e_1_2_10_9_1","first-page":"66","article-title":"Research Progress and Thinking on Genetics and Breeding of Resisitance to Fusarium Head Blingt in Wheat","volume":"18","author":"Gao D. R.","year":"2021","journal-title":"Journal of Yangze University"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1104\/pp.16.01618","article-title":"Two Trichome Birefringence\u2010Like Proteins Mediate Xylan Acetylation, Which Is Essential for Leaf Blight Resistance in Rice","volume":"173","author":"Gao Y.","year":"2017","journal-title":"Plant Physiology"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.3389\/fpls.2012.00012","article-title":"O\u2010Acetylation of Plant Cell Wall Polysaccharides","volume":"3","author":"Gille S.","year":"2012","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"6106","DOI":"10.1021\/jf0006978","article-title":"Cross\u2010Linking of Maize Walls by Ferulate Dimerization and Incorporation Into Lignin","volume":"48","author":"Grabber J. H.","year":"2000","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1094\/PHYTO-03-19-0095-RVW","article-title":"Fusarium Head Blight in Durum Wheat: Recent Status, Breeding Directions, and Future Research Prospects","volume":"109","author":"Haile J. K.","year":"2019","journal-title":"Phytopathology"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1111\/pbi.13790","article-title":"All\u2010In\u2010One: A Robust Fluorescent Fusion Protein Vector Toolbox for Protein Localization and BiFC Analyses in Plants","volume":"20","author":"Han J.","year":"2022","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_10_15_1","first-page":"1995","article-title":"Isochorismate\u2010Based Salicylic Acid Biosynthesis Confers Basal Resistance to Fusarium graminearum in Barley","volume":"8","author":"Hao Q. Q. H. Q.","year":"2018","journal-title":"Molecular Plant Pathology"},{"key":"e_1_2_10_16_1","first-page":"2056","article-title":"Grass Cell Walls: A Story of Cross\u2010Linking","volume":"7","author":"Hatfield R. D.","year":"2016","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1016\/j.cub.2022.04.070","article-title":"Growth\u2013Defense Trade\u2010Offs in Plants","volume":"32","author":"He Z. H.","year":"2022","journal-title":"Current Biology"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"1267","DOI":"10.1093\/mp\/ssu049","article-title":"Growth\u2013Defense Tradeoffs in Plants: A Balancing Act to Optimize Fitness","volume":"7","author":"Huot B.","year":"2014","journal-title":"Molecular Plant"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","first-page":"4382","DOI":"10.1038\/s41467-020-18240-y","article-title":"An Orphan Protein of Fusarium graminearum Modulates Host Immunity by Mediating Proteasomal Degradation of TaSnRK1\u03b1","volume":"11","author":"Jiang C.","year":"2020","journal-title":"Nature Communications"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","first-page":"1014","DOI":"10.1038\/s43016-022-00655-z","article-title":"Emerging Health Threat and Cost of Fusarium Mycotoxins in European Wheat","volume":"3","author":"Johns L. E.","year":"2022","journal-title":"Nature Food"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"1845","DOI":"10.1007\/s11033-019-04637-6","article-title":"Optimization of Agrobacterium\u2010Mediated Transformation in Spring Bread Wheat Using Mature and Immature Embryos","volume":"46","author":"Kumar R.","year":"2019","journal-title":"Molecular Biology Reports"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"521","DOI":"10.1146\/annurev.biochem.76.061005.092322","article-title":"Glycosyltransferases: Structures, Functions, and Mechanisms","volume":"77","author":"Lairson L. L.","year":"2008","journal-title":"Annual Review of Biochemistry"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1111\/nph.14894","article-title":"Fusarium graminearum: Pathogen or Endophyte of North American Grasses?","volume":"217","author":"Lofgren L. A.","year":"2018","journal-title":"New Phytologist"},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"1814","DOI":"10.1111\/jipb.13478","article-title":"A Cell Wall Invertase Modulates Resistance to Fusarium Crown Rot and Sharp Eyespot in Common Wheat","volume":"65","author":"Lv G.","year":"2023","journal-title":"Journal of Integrative Plant Biology"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.15302\/J-FASE-2019262","article-title":"Breeding for the Resistance to Fusarium Head Blight of Wheat in China","volume":"6","author":"Ma H. X.","year":"2019","journal-title":"Frontiers of Agricultural Science and Engineering"},{"key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"5073","DOI":"10.1021\/acs.jafc.3c07277","article-title":"Selenium Improves the Control Efficacy of Phytophthora nicotianae by Damaging the Cell Membrane System and Promoting Plant Energy Metabolism","volume":"72","author":"Ma X. H.","year":"2024","journal-title":"Journal of Agricultural and Food Chemistry"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"1274","DOI":"10.1016\/j.molp.2015.04.007","article-title":"A Robust CRISPR\/Cas9 System for Convenient, High\u2010Efficiency Multiplex Genome Editing in Monocot and Dicot Plants","volume":"8","author":"Ma X. L.","year":"2015","journal-title":"Molecular Plant"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"1541","DOI":"10.1007\/s00122-019-03525-8","article-title":"Germplasms, Genetics and Genomics for Better Control of Disastrous Wheat Fusarium Head Blight","volume":"133","author":"Ma Z. Q.","year":"2020","journal-title":"Theoretical and Applied Genetics"},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fpls.2014.00178","article-title":"The Role of the Cell Wall in Plant Immunity","volume":"5","author":"Malinovsky F. G.","year":"2014","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1016\/j.carres.2015.01.017","article-title":"Hydrolysis of Wheat Flour Arabinoxylan, Acid\u2010Debranched Wheat Flour Arabinoxylan and Arabino\u2010Xylo\u2010Oligosaccharides by \u03b2\u2010Xylanase, \u03b1\u2010L\u2010Arabinofuranosidase and \u03b2\u2010Xylosidase","volume":"407","author":"McCleary B. V.","year":"2015","journal-title":"Carbohydrate Research"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"699","DOI":"10.1016\/j.molp.2024.04.003","article-title":"Plant Cell Wall\u2010Mediated Disease Resistance: Current Understanding and Future Perspectives","volume":"17","author":"Molina A.","year":"2024","journal-title":"Molecular Plant"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/0008-6215(86)80038-6","article-title":"Linkage of p\u2010Coumaroyl and Feruloyl Groups to Cell Wall Polysaccharides of Barley Straw","volume":"148","author":"Mueller\u2010Harvey I.","year":"1986","journal-title":"Carbohydrate Research"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"454","DOI":"10.3390\/molecules26020454","article-title":"Trichothecenes in Food and Feed, Relevance to Human and Animal Health and Methods of Detection: A Systematic Review","volume":"26","author":"Polak\u2010\u015aliwi\u0144ska M.","year":"2021","journal-title":"Molecules"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.copbio.2013.11.013","article-title":"Xylan Biosynthesis","volume":"26","author":"Rennie E. A.","year":"2014","journal-title":"Current Opinion in Biotechnology"},{"key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"686","DOI":"10.1038\/nbt.2650","article-title":"Targeted Genome Modification of Crop Plants Using a CRISPR\/Cas System","volume":"31","author":"Shan Q. W.","year":"2013","journal-title":"Nature Biotechnology"},{"key":"e_1_2_10_36_1","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1146\/annurev-phyto-080615-095835","article-title":"Disease Impact on Wheat Yield Potential and Prospects of Genetic Control","volume":"54","author":"Singh R. P.","year":"2016","journal-title":"Annual Review of Phytopathology"},{"key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1186\/s13068-017-0973-z","article-title":"Designer Biomass for Next\u2010Generation Biorefineries: Leveraging Recent Insights Into Xylan Structure and Biosynthesis","volume":"10","author":"Smith P. J.","year":"2017","journal-title":"Biotechnology for Biofuels"},{"key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"161","DOI":"10.1111\/mpp.12078","article-title":"Sugar Homeostasis Mediated by Cell Wall Invertase GRAIN INCOMPLETE FILLING 1 (GIF1) Plays a Role in Preexisting and Induced Defence in Rice: Cell Wall Invertase Modulates Disease Resistance","volume":"15","author":"Sun L.","year":"2014","journal-title":"Molecular Plant Pathology"},{"key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.tplants.2004.02.005","article-title":"The Role of Plant Cell Wall Polysaccharide Composition in Disease Resistance","volume":"9","author":"Vorwerk S.","year":"2004","journal-title":"Trends in Plant Science"},{"key":"e_1_2_10_40_1","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1038\/s41477-022-01197-9","article-title":"Improving Bread Wheat Yield Through Modulating an Unselected AP2\/ERF Gene","volume":"8","author":"Wang Y.","year":"2022","journal-title":"Nature Plants"},{"key":"e_1_2_10_41_1","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1016\/j.molp.2024.07.008","article-title":"AlphaFold\u2010Guided Redesign of a Plant Pectin Methylesterase Inhibitor for Broad\u2010Spectrum Disease Resistance","volume":"9","author":"Xia Y. Q.","year":"2024","journal-title":"Molecular Plant"},{"key":"e_1_2_10_42_1","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1093\/plphys\/kiab400","article-title":"Knock Out of Transcription Factor WRKY53 Thickens Sclerenchyma Cell Walls, Confers Bacterial Blight Resistance","volume":"187","author":"Xie W.","year":"2021","journal-title":"Plant Physiology"},{"key":"e_1_2_10_43_1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1186\/s42483-022-00142-0","article-title":"Combatting Fusarium Head Blight: Advances in Molecular Interactions Between Fusarium graminearum and Wheat","volume":"4","author":"Xu M.","year":"2022","journal-title":"Phytopathology Research"},{"key":"e_1_2_10_44_1","first-page":"11","article-title":"Enhancing Maize Resistance to Fusarium Verticillioides Through Modulation of Cell Wall Structure and Components by ZmXYXT2","volume":"78","author":"Xu Y.","year":"2025","journal-title":"Journal of Advanced Research"},{"key":"e_1_2_10_45_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.plantsci.2022.111476","article-title":"Outstanding Questions on Xylan Biosynthesis","volume":"325","author":"Ye Z. H.","year":"2022","journal-title":"Plant Science"},{"key":"e_1_2_10_46_1","doi-asserted-by":"crossref","first-page":"2197","DOI":"10.1093\/plphys\/kiad665","article-title":"Xylan\u2010Directed Cell Wall Assembly in Grasses","volume":"194","author":"Zhang L.","year":"2023","journal-title":"Plant Physiology"},{"key":"e_1_2_10_47_1","doi-asserted-by":"crossref","first-page":"e00789","DOI":"10.1128\/msystems.00789-20","article-title":"Elucidation of the Pathogenicity\u2010Associated Regulatory Network in Xanthomonas oryzae pv. Oryzae","volume":"6","author":"Zheng D. H.","year":"2021","journal-title":"mSystems"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70747","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70747","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70747","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T09:47:59Z","timestamp":1787564879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70747"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,24]]},"references-count":46,"alternative-id":["10.1111\/pbi.70747"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70747","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,24]]},"assertion":[{"value":"2025-12-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pbi.70747"}}