{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T19:21:34Z","timestamp":1787512894617,"version":"build-2736575974"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T00:00:00Z","timestamp":1787443200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"ABSTRACT<\/jats:title>\n \n As a major commercial legume crop, soybean ranks among the world's most significant sources of edible oil and plant protein. We previously identified a\n SEIPIN<\/jats:italic>\n homologue (\n FA9<\/jats:italic>\n ) at the\n fatty acid 9<\/jats:italic>\n locus that promotes fatty acid accumulation in soybean. To examine the detailed molecular mechanisms by which\n FA9<\/jats:italic>\n regulates lipid metabolism, we performed single\u2010cell RNA sequencing (scRNA\u2010seq) and spatial transcriptomics (stRNA\u2010seq) of wild\u2010type and\n FA9<\/jats:italic>\n \u2010knockout soybean seeds at the late maturity stage. scRNA\u2010seq analysis identified 26 transcriptional clusters and revealed the spatial distribution of\n FA9<\/jats:italic>\n in seeds, in which the deletion of\n FA9<\/jats:italic>\n altered lipid and storage\u2010related transcriptional programmes. On the basis of single\u2010cell sequencing and immunoprecipitation\u2013mass spectrometry (IP\u2013MS), the vesicle\u2010associated membrane protein (VAMP)\u2010associated protein (VAP) was identified, and subsequent experiments demonstrated that FA9 interacts specifically with VAP via its N\u2010terminal FFAT motif at the endoplasmic reticulum. Seeds of\n vap<\/jats:italic>\n knockout (\n vap\u2010KO1<\/jats:italic>\n and\n vap\u2010KO2<\/jats:italic>\n ) and\n fa9 vap<\/jats:italic>\n double knockout (\n fa9 vap\u2010KO<\/jats:italic>\n ) lines, created by CRISPR\u2013Cas9 gene editing, had higher protein contents and lower total fatty acid contents than wild\u2010type soybean, whereas overexpression of\n FA9<\/jats:italic>\n and\n VAP<\/jats:italic>\n enhanced lipid droplet formation in\n Nicotiana benthamiana<\/jats:italic>\n . These findings reveal that FA9 interacts with VAP to promote lipid droplet biogenesis and lipid transport, thereby driving fatty acid accumulation in soybean seeds. This research provides new insight into the molecular mechanisms that regulate seed oil synthesis and identifies potential target genes for improvement of soybean oil quality through molecular breeding.\n <\/jats:p>","DOI":"10.1111\/pbi.70750","type":"journal-article","created":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T19:19:31Z","timestamp":1787512771000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Transcriptomics Reveals the\n FA9<\/scp>\n \u2013\n VAP<\/scp>\n Module Regulating Fatty Acid Accumulation in Soybean Seeds"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2967-0094","authenticated-orcid":false,"given":"Hui","family":"Li","sequence":"first","affiliation":[{"name":"National Key Laboratory of Smart Farm Technology and System, National Research Center of Soybean Engineering and Technology Northeast Agricultural University Harbin Heilongjiang 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Agricultural University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Wang","sequence":"additional","affiliation":[{"name":"Suileng County Agricultural Technology Extension Center Suihua Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0873-6456","authenticated-orcid":false,"given":"Xue","family":"Han","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Smart Farm Technology and System, National Research Center of Soybean Engineering and Technology Northeast Agricultural University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9193-4738","authenticated-orcid":false,"given":"Sui","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Smart Farm Technology and System, National Research Center of Soybean Engineering and Technology Northeast Agricultural University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3714-9066","authenticated-orcid":false,"given":"Qingshan","family":"Chen","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Smart Farm Technology and System, National Research Center of Soybean Engineering and Technology Northeast Agricultural University Harbin Heilongjiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0657-9127","authenticated-orcid":false,"given":"Zhaoming","family":"Qi","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Smart Farm Technology and System, National Research Center of Soybean Engineering and Technology Northeast Agricultural University Harbin Heilongjiang 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