{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T07:16:16Z","timestamp":1788419776099,"version":"build-2803163510"},"reference-count":60,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:00:00Z","timestamp":1788307200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Developing barley grains must balance the use of imported sucrose between soluble sugars and storage starch, but how this balance is controlled remains unclear. Here we show that loss of the plastidial \u03b1\u2010glucan phosphorylase\n                    <jats:italic>HvPHO1<\/jats:italic>\n                    causes starch and soluble sugars to accumulate concurrently in barley grains. Developing\n                    <jats:italic>pho1<\/jats:italic>\n                    grains showed sustained increases in glucose 1\u2010phosphate (G1P) and adenosine diphosphate glucose (ADP\u2010glucose), and exogenous G1P also promoted starch accumulation, supporting a stimulatory effect of elevated G1P on starch synthesis. G1P directly bound sucrose non\u2010fermenting 1\u2010related protein kinase 1 (HvSnRK1), suppressing its kinase activity and its phosphorylation of sucrose synthase 2 (HvSUSY2); the metabolic phenotypes of\n                    <jats:italic>snrk1<\/jats:italic>\n                    and\n                    <jats:italic>susy2<\/jats:italic>\n                    mutants supported a role for this phosphorylation module in sucrose utilization and soluble\u2010sugar homeostasis. HvPHO1 also interacted with the starch\u2010granule initiation factor starch synthase 4 (HvSS4). Loss of\n                    <jats:italic>HvPHO1<\/jats:italic>\n                    increased the abundance of B\u2010type starch granules and shifted their size distribution, indicating a parallel role in storage\u2010starch organization.\n                    <jats:italic>pho1<\/jats:italic>\n                    lines showed greater grain dry weight and test weight. Thus, loss of\n                    <jats:italic>HvPHO1<\/jats:italic>\n                    initiates G1P\u2010mediated metabolic feedback that reconfigures the balance between sucrose utilization and starch accumulation and is associated with enhanced grain carbon deposition and grain weight. These findings reveal G1P\u2010dependent feedback linking starch\u2010precursor status to SnRK1\u2010mediated carbon allocation in barley endosperm.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70754","type":"journal-article","created":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T06:45:32Z","timestamp":1788417932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>HvPHO1<\/i>\n                    Controls a Glucose 1\u2010Phosphate\u2013\n                    <scp>SnRK1<\/scp>\n                    Signalling Module That Coordinates Starch Accumulation in Barley Endosperm"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4846-2290","authenticated-orcid":false,"given":"Yulong","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu 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Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7372-6596","authenticated-orcid":false,"given":"Yazhou","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanlin","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7772-9591","authenticated-orcid":false,"given":"Pengfei","family":"Qi","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mei","family":"Deng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shoufen","family":"Dai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6265-7911","authenticated-orcid":false,"given":"Jian","family":"Ma","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7928-9334","authenticated-orcid":false,"given":"Guoyue","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jirui","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae Research Institute Sichuan Agricultural University  Chengdu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youliang","family":"Zheng","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Triticeae 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