{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:59:32Z","timestamp":1790085572177,"version":"4.0.1"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T00:00:00Z","timestamp":1790035200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFF1003203"],"award-info":[{"award-number":["2022YFF1003203"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012421","name":"Agricultural Science and Technology Innovation Program","doi-asserted-by":"publisher","award":["ASTIP\u2010IAS14"],"award-info":[{"award-number":["ASTIP\u2010IAS14"]}],"id":[{"id":"10.13039\/501100012421","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Biotechnology Journal"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Drought constitutes a major abiotic stress limiting alfalfa productivity. To elucidate the genetic basis of drought tolerance, a genome\u2010wide association study (GWAS) was conducted in alfalfa, which identified a CBF\/DREB1 family transcription factor designated MsCBF8. Utilizing an optimized CRISPR\u2010Cas9 system, mutants were successfully generated in the complex autotetraploid background. Combined with overexpression and Arabidopsis complementation assays, this confirmed that MsCBF8 functions as a positive regulator of drought tolerance, with its overexpression enhancing drought tolerance without compromising plant growth. Physiological analyses revealed that MsCBF8 improves drought tolerance by coordinating antioxidant defence (elevated SOD and CAT activities, decreased H\n                    <jats:sub>2<\/jats:sub>\n                    O\n                    <jats:sub>2<\/jats:sub>\n                    and MDA contents), dynamically regulating stomatal movement (increased water\u2010use efficiency), and maintaining photosynthetic performance. At the molecular level, MsCBF8 directly and specifically binds to the GCCGAC\n                    <jats:italic>cis<\/jats:italic>\n                    \u2010element in the\n                    <jats:italic>MsGolS2<\/jats:italic>\n                    promoter and activates its expression. Silencing\n                    <jats:italic>MsGolS2<\/jats:italic>\n                    via virus\u2010induced gene silencing (VIGS) reduced drought tolerance and aggravated oxidative damage, providing the first functional evidence for the essential role of this gene in the drought response of alfalfa. This study systematically elucidates a novel MsCBF8\u2010\n                    <jats:italic>MsGolS2<\/jats:italic>\n                    regulatory module, offering crucial insights into the drought adaptation mechanisms in alfalfa and providing valuable genetic resources and breeding targets for developing high\u2010yield, water\u2010efficient and drought\u2010tolerant alfalfa cultivars.\n                  <\/jats:p>","DOI":"10.1111\/pbi.70756","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:01:23Z","timestamp":1790082083000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Converging\n                    <scp>GWAS<\/scp>\n                    and Genome Editing Decrypts\n                    <scp>MsCBF8<\/scp>\n                    \u2010\n                    <scp>\n                      <i>MsGolS2<\/i>\n                    <\/scp>\n                    Regulatory Module for Drought Tolerance Without Yield Penalty in\n                    <scp>\n                      <i>Medicago sativa<\/i>\n                    <\/scp>"],"prefix":"10.1111","author":[{"given":"Yangyang","family":"Zhang","sequence":"first","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueqian","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2412-1639","authenticated-orcid":false,"given":"Huajuan","family":"Leng","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Cui","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Jiang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lili","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Horticulture University of Nebraska\u2010Lincoln  Lincoln Nebraska USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiejun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingchuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2902-1393","authenticated-orcid":false,"given":"Xue","family":"Wang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1051-9028","authenticated-orcid":false,"given":"Junmei","family":"Kang","sequence":"additional","affiliation":[{"name":"Institute of Animal Science Chinese Academy of Agricultural Sciences  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,22]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.plaphy.2016.12.025","article-title":"Oil Palm Drought Inducible DREB1 Induced Expression of DRE\/CRT\u2010 and Non\u2010DRE\/CRT\u2010Containing Genes in Lowland Transgenic Tomato Under Cold and PEG Treatments","volume":"112","author":"Azzeme A. M.","year":"2017","journal-title":"Plant Physiology and Biochemistry"},{"key":"e_1_2_10_3_1","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1007\/s10725-009-9434-4","article-title":"DREB1C From Medicago truncatula Enhances Freezing Tolerance in Transgenic M. truncatula and China Rose (Rosa chinensis Jacq.)","volume":"60","author":"Chen J.","year":"2010","journal-title":"Plant Growth Regulation"},{"key":"e_1_2_10_4_1","doi-asserted-by":"crossref","DOI":"10.1093\/plphys\/kiaf428","article-title":"Natural Allelic Variation in the CBF2 Transcription Factor Is a Pivotal Factor Controlling Cold Resistance in Potato","volume":"199","author":"Chen Y.","year":"2025","journal-title":"Plant Physiology"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1046\/j.1365-313x.1998.00343.x","article-title":"Floral Dip: A Simplified Method for Agrobacterium\u2010Mediated Transformation of \n                     Arabidopsis thaliana","volume":"16","author":"Clough S. J.","year":"1998","journal-title":"Plant Journal"},{"key":"e_1_2_10_6_1","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1186\/s12870-025-06081-0","article-title":"Genome\u2010Wide Identification and Expression Analysis of CBF\/DREB1 Gene Family in \n                     Medicago sativa\n                   L. and Functional Verification of MsCBF9 Affecting Flowering Time","volume":"25","author":"Cui J.","year":"2025","journal-title":"BMC Plant Biology"},{"key":"e_1_2_10_7_1","doi-asserted-by":"crossref","DOI":"10.3390\/ijms25168942","article-title":"Physiological, Metabolome and Gene Expression Analyses Reveal the Accumulation and Biosynthesis Pathways of Soluble Sugars and Amino Acids in Sweet Sorghum Under Osmotic Stresses","volume":"25","author":"Cui Y. N.","year":"2024","journal-title":"International Journal of Molecular Sciences"},{"key":"e_1_2_10_8_1","doi-asserted-by":"crossref","first-page":"251","DOI":"10.1007\/s00299-024-03311-x","article-title":"Evaluation of Oxidative Stress, Biochemical Parameters and In Silico Markers in Different Pea Accessions in Response to Drought Stress","volume":"43","author":"Dutta A.","year":"2024","journal-title":"Plant Cell Reports"},{"key":"e_1_2_10_9_1","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.plaphy.2021.10.033","article-title":"Membrane Remodelling and Triacylglycerol Accumulation in Drought Stress Resistance: The Case Study of Soybean Phospholipases A","volume":"169","author":"Ferreira D.","year":"2021","journal-title":"Plant Physiology and Biochemistry"},{"key":"e_1_2_10_10_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1007\/978-1-4939-1695-5_17","article-title":"Alfalfa (\n                     Medicago sativa\n                   L.)","volume":"1223","author":"Fu C.","year":"2015","journal-title":"Methods in Molecular Biology"},{"key":"e_1_2_10_11_1","doi-asserted-by":"crossref","first-page":"157","DOI":"10.1007\/s11103-015-0403-1","article-title":"ZmGOLS2, a Target of Transcription Factor ZmDREB2A, Offers Similar Protection Against Abiotic Stress as ZmDREB2A","volume":"90","author":"Gu L.","year":"2016","journal-title":"Plant Molecular Biology"},{"key":"e_1_2_10_12_1","doi-asserted-by":"crossref","first-page":"677","DOI":"10.1093\/plphys\/kiab050","article-title":"Transcription\u2010Associated Metabolomic Adjustments in Maize Occur During Combined Drought and Cold Stress","volume":"186","author":"Guo Q.","year":"2021","journal-title":"Plant Physiology"},{"key":"e_1_2_10_13_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1126\/science.aaz7614","article-title":"The Physiology of Plant Responses to Drought","volume":"368","author":"Gupta A.","year":"2020","journal-title":"Science"},{"key":"e_1_2_10_14_1","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1111\/tpj.17012","article-title":"Trait Association and Prediction Through Integrative k\u2010Mer Analysis","volume":"120","author":"He C.","year":"2024","journal-title":"Plant Journal"},{"key":"e_1_2_10_15_1","doi-asserted-by":"crossref","first-page":"1262","DOI":"10.1038\/s41588-025-02164-8","article-title":"Pan\u2010Genomic Analysis Highlights Genes Associated With Agronomic Traits and Enhances Genomics\u2010Assisted Breeding in Alfalfa","volume":"57","author":"He F.","year":"2025","journal-title":"Nature Genetics"},{"key":"e_1_2_10_16_1","doi-asserted-by":"crossref","first-page":"2133","DOI":"10.1111\/nph.20391","article-title":"Manipulating Stomatal Aperture by Silencing StSLAC1 Affects Potato Plant\u2010Herbivore\u2010Parasitoid Tritrophic Interactions Under Drought Stress","volume":"245","author":"He X.","year":"2025","journal-title":"New Phytologist"},{"key":"e_1_2_10_17_1","doi-asserted-by":"crossref","DOI":"10.1093\/hr\/uhae271","article-title":"The Whole\u2010Genome Dissection of Root System Architecture Provides New Insights for the Genetic Improvement of Alfalfa (\n                     Medicago sativa\n                   L.)","volume":"12","author":"Jiang X.","year":"2025","journal-title":"Horticulture Research"},{"key":"e_1_2_10_18_1","doi-asserted-by":"crossref","first-page":"922","DOI":"10.1016\/j.tplants.2022.01.008","article-title":"Transcriptional Regulatory Network of Plant Cold\u2010Stress Responses","volume":"27","author":"Kidokoro S.","year":"2022","journal-title":"Trends in Plant Science"},{"key":"e_1_2_10_19_1","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1093\/plphys\/kiae105","article-title":"Regulatory Networks in Plant Responses to Drought and Cold Stress","volume":"195","author":"Kim J.","year":"2024","journal-title":"Plant Physiology"},{"key":"e_1_2_10_20_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.jplph.2022.153806","article-title":"ScCBF1 Plays a Stronger Role in Cold, Salt and Drought Tolerance Than StCBF1 in Potato (\n                     Solanum tuberosum\n                  )","volume":"278","author":"Li C.","year":"2022","journal-title":"Journal of Plant Physiology"},{"key":"e_1_2_10_21_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1016\/j.plantsci.2018.01.015","article-title":"Ectopic Expression of StCBF1 and ScCBF1 Have Different Functions in Response to Freezing and Drought Stresses in Arabidopsis","volume":"270","author":"Li J.","year":"2018","journal-title":"Plant Science"},{"key":"e_1_2_10_22_1","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1023\/A:1013316816955","article-title":"Bar\u2010Expressing Peppermint (Mentha \u00d7 Piperita L. Var. Black Mitcham) Plants Are Highly Resistant to the Glufosinate Herbicide Liberty","volume":"8","author":"Li X.","year":"2001","journal-title":"Molecular Breeding"},{"key":"e_1_2_10_23_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.plaphy.2023.107999","article-title":"MsCYP71 Is a Positive Regulator for Drought Resistance in Alfalfa","volume":"203","author":"Liu J.","year":"2023","journal-title":"Plant Physiology and Biochemistry"},{"key":"e_1_2_10_24_1","doi-asserted-by":"crossref","first-page":"1391","DOI":"10.1105\/tpc.10.8.1391","article-title":"Two Transcription Factors, DREB1 and DREB2, With an EREBP\/AP2 DNA Binding Domain Separate Two Cellular Signal Transduction Pathways in Drought\u2010 and Low\u2010Temperature\u2010Responsive Gene Expression, Respectively, in Arabidopsis","volume":"10","author":"Liu Q.","year":"1998","journal-title":"Plant Cell"},{"key":"e_1_2_10_25_1","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1111\/tpj.16116","article-title":"Raffinose Positively Regulates Maize Drought Tolerance by Reducing Leaf Transpiration","volume":"114","author":"Liu Y.","year":"2023","journal-title":"Plant Journal"},{"key":"e_1_2_10_26_1","doi-asserted-by":"crossref","first-page":"429","DOI":"10.1111\/tpj.15955","article-title":"Nuclear Transport Factor 2\u2010Like Improves Drought Tolerance by Modulating Leaf Water Loss in Alfalfa (\n                     Medicago sativa\n                   L.)","volume":"112","author":"Luo D.","year":"2022","journal-title":"Plant Journal"},{"key":"e_1_2_10_27_1","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1016\/j.plantsci.2016.01.005","article-title":"Gamma\u2010Aminobutyric Acid Depletion Affects Stomata Closure and Drought Tolerance of \n                     Arabidopsis thaliana","volume":"245","author":"Mekonnen D. W.","year":"2016","journal-title":"Plant Science"},{"key":"e_1_2_10_28_1","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1016\/j.plaphy.2018.08.015","article-title":"COP1 Plays a Prominent Role in Drought Stress Tolerance in Arabidopsis and Pea","volume":"130","author":"Moazzam\u2010Jazi M.","year":"2018","journal-title":"Plant Physiology and Biochemistry"},{"key":"e_1_2_10_29_1","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1086\/519795","article-title":"PLINK: A Tool Set for Whole\u2010Genome Association and Population\u2010Based Linkage Analyses","volume":"81","author":"Purcell S.","year":"2007","journal-title":"American Journal of Human Genetics"},{"key":"e_1_2_10_30_1","doi-asserted-by":"crossref","first-page":"805","DOI":"10.1111\/jipb.12654","article-title":"Reactive Oxygen Species Signaling and Stomatal Movement in Plant Responses to Drought Stress and Pathogen Attack","volume":"60","author":"Qi J.","year":"2018","journal-title":"Journal of Integrative Plant Biology"},{"key":"e_1_2_10_31_1","doi-asserted-by":"crossref","first-page":"669","DOI":"10.1080\/07388551.2021.1874280","article-title":"Drought Stress\u2010Induced Physiological Mechanisms, Signaling Pathways and Molecular Response of Chloroplasts in Common Vegetable Crops","volume":"41","author":"Razi K.","year":"2021","journal-title":"Critical Reviews in Biotechnology"},{"key":"e_1_2_10_32_1","doi-asserted-by":"crossref","first-page":"1873","DOI":"10.1111\/tpj.16612","article-title":"Complex Plant Responses to Drought and Heat Stress Under Climate Change","volume":"117","author":"Sato H.","year":"2024","journal-title":"Plant Journal"},{"key":"e_1_2_10_33_1","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1038\/35066500","article-title":"Guard Cell Abscisic Acid Signalling and Engineering Drought Hardiness in Plants","volume":"410","author":"Schroeder J. I.","year":"2001","journal-title":"Nature"},{"key":"e_1_2_10_34_1","doi-asserted-by":"crossref","first-page":"1757","DOI":"10.1111\/pbi.14300","article-title":"The Chromosome\u2010Level Assembly of the Wild Diploid Alfalfa Genome Provides Insights Into the Full Landscape of Genomic Variations Between Cultivated and Wild Alfalfa","volume":"22","author":"Shi K.","year":"2024","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_10_35_1","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/jipb.13626","article-title":"An Alfalfa MYB\u2010Like Transcriptional Factor MsMYBH Positively Regulates Alfalfa Seedling Drought Resistance and Undergoes MsWAV3\u2010Mediated Degradation","volume":"66","author":"Shi K.","year":"2024","journal-title":"Journal of Integrative Plant Biology"},{"key":"e_1_2_10_36_1","article-title":"Identification of Differential Drought Response Mechanisms in Medicago sativa Subsp. sativa and falcata Through Comparative Assessments at the Physiological, Biochemical, and Transcriptional Levels","volume":"10","author":"Singer S. D.","year":"2021","journal-title":"Plants (Basel)"},{"key":"e_1_2_10_37_1","doi-asserted-by":"crossref","first-page":"4265","DOI":"10.1038\/s41467-022-31844-w","article-title":"Natural Variation of DROT1 Confers Drought Adaptation in Upland Rice","volume":"13","author":"Sun X.","year":"2022","journal-title":"Nature Communications"},{"key":"e_1_2_10_38_1","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1046\/j.0960-7412.2001.01227.x","article-title":"Important Roles of Drought\u2010 and Cold\u2010Inducible Genes for Galactinol Synthase in Stress Tolerance in \n                     Arabidopsis thaliana","volume":"29","author":"Taji T.","year":"2002","journal-title":"Plant Journal"},{"key":"e_1_2_10_39_1","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1038\/s41588-020-0612-7","article-title":"Identifying Genetic Variants Underlying Phenotypic Variation in Plants Without Complete Genomes","volume":"52","author":"Voichek Y.","year":"2020","journal-title":"Nature Genetics"},{"key":"e_1_2_10_40_1","doi-asserted-by":"crossref","DOI":"10.3389\/fpls.2022.851731","article-title":"Dehydration\u2010Responsive Element Binding Protein 1C, 1E, and 1G Promote Stress Tolerance to Chilling, Heat, Drought, and Salt in Rice","volume":"13","author":"Wang H.","year":"2022","journal-title":"Frontiers in Plant Science"},{"key":"e_1_2_10_41_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1111\/nph.17593","article-title":"JMJ27\u2010Mediated Histone H3K9 Demethylation Positively Regulates Drought\u2010Stress Responses in Arabidopsis","volume":"232","author":"Wang Q.","year":"2021","journal-title":"New Phytologist"},{"key":"e_1_2_10_42_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.123255","article-title":"GmDof41 Regulated by the DREB1\u2010Type Protein Improves Drought and Salt Tolerance by Regulating the DREB2\u2010Type Protein in Soybean","volume":"230","author":"Wei J. T.","year":"2023","journal-title":"International Journal of Biological Macromolecules"},{"key":"e_1_2_10_43_1","doi-asserted-by":"crossref","DOI":"10.1093\/hr\/uhac275","article-title":"MaDREB1F Confers Cold and Drought Stress Resistance Through Common Regulation of Hormone Synthesis and Protectant Metabolite Contents in Banana","volume":"10","author":"Xu Y.","year":"2022","journal-title":"Horticulture Research"},{"key":"e_1_2_10_44_1","doi-asserted-by":"crossref","first-page":"1452","DOI":"10.1111\/pbi.14597","article-title":"Overexpression of MtNAC33 Enhances Biomass Yield and Drought Tolerance in Alfalfa","volume":"23","author":"Yang R.","year":"2025","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_10_45_1","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1111\/pbi.13770","article-title":"Construction of Genic Male Sterility System by CRISPR\/Cas9 Editing From Model Legume to Alfalfa","volume":"20","author":"Ye Q.","year":"2022","journal-title":"Plant Biotechnology Journal"},{"key":"e_1_2_10_46_1","doi-asserted-by":"crossref","DOI":"10.1093\/hr\/uhac225","article-title":"Application of Machine Learning to Explore the Genomic Prediction Accuracy of Fall Dormancy in Autotetraploid Alfalfa","volume":"10","author":"Zhang F.","year":"2023","journal-title":"Horticulture Research"},{"key":"e_1_2_10_47_1","doi-asserted-by":"crossref","first-page":"1339","DOI":"10.1016\/j.molp.2023.08.001","article-title":"The Long Non\u2010Coding RNA DANA2 Positively Regulates Drought Tolerance by Recruiting ERF84 to Promote JMJ29\u2010Mediated Histone Demethylation","volume":"16","author":"Zhang P.","year":"2023","journal-title":"Molecular Plant"},{"key":"e_1_2_10_48_1","doi-asserted-by":"crossref","DOI":"10.1016\/j.ijbiomac.2023.125231","article-title":"The DREB Transcription Factor, a Biomacromolecule, Responds to Abiotic Stress by Regulating the Expression of Stress\u2010Related Genes","volume":"243","author":"Zhang Y.","year":"2023","journal-title":"International Journal of Biological Macromolecules"},{"key":"e_1_2_10_49_1","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.cj.2024.04.001","article-title":"Development of a Single Transcript CRISPR\/Cas9 Toolkit for Efficient Genome Editing in Autotetraploid Alfalfa","volume":"12","author":"Zhao H.","year":"2024","journal-title":"Crop Journal"},{"key":"e_1_2_10_50_1","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1038\/ng.2310","article-title":"Genome\u2010Wide Efficient Mixed\u2010Model Analysis for Association Studies","volume":"44","author":"Zhou X.","year":"2012","journal-title":"Nature Genetics"},{"key":"e_1_2_10_51_1","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1093\/jxb\/erz569","article-title":"Overexpression of Soybean DREB1 Enhances Drought Stress Tolerance of Transgenic Wheat in the Field","volume":"71","author":"Zhou Y.","year":"2020","journal-title":"Journal of Experimental Botany"}],"container-title":["Plant Biotechnology Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70756","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pbi.70756","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pbi.70756","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:01:30Z","timestamp":1790082090000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pbi.70756"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,22]]},"references-count":50,"alternative-id":["10.1111\/pbi.70756"],"URL":"https:\/\/doi.org\/10.1111\/pbi.70756","archive":["Portico"],"relation":{},"ISSN":["1467-7644","1467-7652"],"issn-type":[{"value":"1467-7644","type":"print"},{"value":"1467-7652","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,22]]},"assertion":[{"value":"2025-06-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-08-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-22","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"pbi.70756"}}