{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T09:37:41Z","timestamp":1778665061886,"version":"3.51.4"},"reference-count":53,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Cell & Environment"],"published-print":{"date-parts":[[2014,7]]},"abstract":"Abstract<\/jats:title>Copper is an essential micronutrient for plant growth and development, and copper transporter plays a pivotal role for keeping copper homeostasis. However, little is known about copper transporters in wheat. Here, we report a novel copper transporter gene family, TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic>, in common wheat. Three TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> homoeologous genes were isolated and assigned to group 5 chromosomes. Each of the TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> genes (TaCT<\/jats:italic><\/jats:styled-content>1\u20105<\/jats:italic>A<\/jats:italic><\/jats:styled-content>, \u22125<\/jats:italic>B<\/jats:italic><\/jats:styled-content> or \u22125<\/jats:italic>D<\/jats:italic><\/jats:styled-content>) possesses 12 transmembrane domains. TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> genes exhibited higher transcript levels in leaf than in root, culm and spikelet. Excess copper down\u2010regulated the transcript levels of TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> and copper deficiency\u2010induced TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> expression. Subcellular experiments localized the TaCT<\/jats:styled-content>1 to the G<\/jats:styled-content>olgi apparatus. Yeast expression experiments and virus\u2010induced gene silencing analysis indicated that the TaCT<\/jats:styled-content>1 functioned in copper transport. Site\u2010directed mutagenesis demonstrated that three amino acid residues, M<\/jats:styled-content>et35<\/jats:sup>, M<\/jats:styled-content>et38<\/jats:sup> and C<\/jats:styled-content>ys365<\/jats:sup>, are required for TaCT<\/jats:styled-content>1 function. Phylogenetic and functional analyses suggested that homologous genes shared high similarity with TaCT<\/jats:italic><\/jats:styled-content>1<\/jats:italic> may exist exclusively in monocot plants. Our work reveals a novel wheat gene family encoding major facilitator superfamily (MFS<\/jats:styled-content>)\u2010type copper transporters, and provides evidence for their functional involvement in promoting copper uptake and keeping copper homeostasis in common wheat.<\/jats:p>","DOI":"10.1111\/pce.12263","type":"journal-article","created":{"date-parts":[[2013,12,25]],"date-time":"2013-12-25T02:58:43Z","timestamp":1387940323000},"page":"1561-1573","source":"Crossref","is-referenced-by-count":22,"title":["Identification and characterization of a novel copper transporter gene family TaCT<\/i><\/scp>1<\/i> in common wheat"],"prefix":"10.1111","volume":"37","author":[{"given":"HAOXUN","family":"LI","sequence":"first","affiliation":[{"name":"Institute of Genetics and Developmental Biology, Chinese Academy of Sciences The State Key Laboratory of Plant Cell and Chromosome Engineering Beijing 100101 China"},{"name":"University of Chinese Academy of Sciences Beijing 100049 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