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When grown under <jats:italic>in vitro<\/jats:italic> conditions, seedlings with mutations in <jats:styled-content style=\"fixed-case\"><jats:italic>A<\/jats:italic><\/jats:styled-content><jats:italic>rabidopsis thaliana<\/jats:italic> genes <jats:styled-content style=\"fixed-case\"><jats:italic>MLO<\/jats:italic><\/jats:styled-content><jats:italic>4<\/jats:italic> or <jats:styled-content style=\"fixed-case\"><jats:italic>MLO<\/jats:italic><\/jats:styled-content><jats:italic>11<\/jats:italic> exhibit aberrant root growth patterns upon contact with hard surfaces, exemplified as tight root spirals. We used a set of physiological assays and genetic tools to characterize this thigmomorphogenic defect in detail. We observed that the <jats:italic>mlo4<\/jats:italic>\/<jats:italic>mlo11<\/jats:italic>\u2010associated root curling phenotype is not recapitulated in a set of mutants with altered root growth patterns or architecture. We further found that <jats:italic>mlo4<\/jats:italic>\/<jats:italic>mlo11<\/jats:italic>\u2010conditioned root curling is not dependent upon light and endogenous flavonoids, but is <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content>\u2010sensitive and affected by exogenous calcium levels. Based upon the latter two characteristics, <jats:italic>mlo4<\/jats:italic>\u2010associated root coiling appears to be mechanistically different from the natural strong root curvature of the <jats:styled-content style=\"fixed-case\">A<\/jats:styled-content>rabidopsis ecotype <jats:styled-content style=\"fixed-case\">L<\/jats:styled-content>andsberg <jats:italic>erecta<\/jats:italic>. Gravistimulation reversibly overrides the aberrant thigmomorphogenesis of <jats:italic>mlo4<\/jats:italic> seedlings. Mutants with dominant negative defects in <jats:italic>\u03b1<\/jats:italic>\u2010tubulin modulate the extent and directionality of <jats:italic>mlo4<\/jats:italic>\/<jats:italic>mlo11<\/jats:italic>\u2010conditioned root coils, whereas mutants defective in polar auxin transport (<jats:italic>axr4<\/jats:italic>, <jats:italic>aux1<\/jats:italic>) or gravitropism (<jats:italic>pgm1<\/jats:italic>) completely suppress the <jats:italic>mlo4<\/jats:italic> root curling phenotype. Our data implicate a joint contribution of calcium signalling, <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> regulation, microtubular function, polar auxin transport and gravitropism in root thigmomorphogenesis.<\/jats:p>","DOI":"10.1111\/pce.12353","type":"journal-article","created":{"date-parts":[[2014,4,16]],"date-time":"2014-04-16T15:41:31Z","timestamp":1397662891000},"page":"2738-2753","source":"Crossref","is-referenced-by-count":58,"title":["Physiological characterization and genetic modifiers of aberrant root thigmomorphogenesis in mutants of <scp><i>A<\/i><\/scp><i>rabidopsis thaliana<\/i>\u2005<scp><i>MILDEW LOCUS O<\/i><\/scp> genes"],"prefix":"10.1111","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6057-2575","authenticated-orcid":false,"given":"PRZEMYSLAW","family":"BIDZINSKI","sequence":"first","affiliation":[{"name":"Department of Plant\u2010Microbe Interactions Max\u2010Planck\u2010Institute for Plant Breeding Research  50829 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"DOMINIKA MARTA","family":"GRATKOWSKA","sequence":"additional","affiliation":[{"name":"Department of Plant\u2010Microbe Interactions Max\u2010Planck\u2010Institute for Plant Breeding Research  50829 Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"RALPH","family":"PANSTRUGA","sequence":"additional","affiliation":[{"name":"Department of Plant\u2010Microbe Interactions Max\u2010Planck\u2010Institute for Plant Breeding Research  50829 Cologne Germany"},{"name":"Institute for Biology I Unit of Plant Molecular Cell Biology RWTH Aachen University  52056 Aachen 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