{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T02:02:50Z","timestamp":1789178570980,"version":"build-2803163510"},"reference-count":71,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,9,13]],"date-time":"2017-09-13T00:00:00Z","timestamp":1505260800000},"content-version":"am","delay-in-days":334,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#am"},{"start":{"date-parts":[[2016,10,14]],"date-time":"2016-10-14T00:00:00Z","timestamp":1476403200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["CE140100008"],"award-info":[{"award-number":["CE140100008"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["DP0771413"],"award-info":[{"award-number":["DP0771413"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000923","name":"Australian Research Council","doi-asserted-by":"publisher","award":["CE140100015"],"award-info":[{"award-number":["CE140100015"]}],"id":[{"id":"10.13039\/501100000923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The role of some aquaporins as CO<jats:sub>2<\/jats:sub> permeable channels has been controversial. Low CO<jats:sub>2<\/jats:sub> permeability of plant membranes has been criticized because of unstirred layers and other limitations. Here we measured both water and CO<jats:sub>2<\/jats:sub> permeability (<jats:italic>P<jats:sub>os<\/jats:sub><\/jats:italic>, <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic>) using stopped flow on plasma membrane vesicles (pmv) isolated from <jats:italic>Pisum sativum<\/jats:italic> (pea) and <jats:italic>Arabidopsis thaliana<\/jats:italic> leaves. We excluded the chemical limitation of carbonic anhydrase (CA) in the vesicle acidification technique for <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic> using different temperatures and CA concentrations. Unstirred layers were excluded based on small vesicle size and the positive correlation between vesicle diameter and <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic>. We observed high aquaporin activity (<jats:italic>P<jats:sub>os<\/jats:sub><\/jats:italic> 0.06 to 0.22\u2009cm\u2009s<jats:sup>\u22121<\/jats:sup>) for pea pmv based on all the criteria for their function using inhibitors and temperature dependence. Inhibitors of <jats:italic>P<jats:sub>os<\/jats:sub><\/jats:italic> did not alter <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic>. <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic> ranged from 0.001 to 0.012\u2009cm\u2009s<jats:sup>\u22121<\/jats:sup> (mean 0.0079\u2009<jats:styled-content>+<\/jats:styled-content>\u20090.0007\u2009cm\u2009s<jats:sup>\u22121<\/jats:sup>) with activation energy of 30.2\u2009kJ\u2009mol<jats:sup>\u22121<\/jats:sup>. Intrinsic variation between pmv batches from normally grown or stressed plants revealed a weak (<jats:italic>R<\/jats:italic><jats:sup>2<\/jats:sup>\u2009=\u20090.27) positive linear correlation between <jats:italic>P<jats:sub>os<\/jats:sub><\/jats:italic> and <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic>. Despite the low <jats:italic>P<jats:sub>CO2<\/jats:sub><\/jats:italic>, aquaporins may facilitate CO<jats:sub>2<\/jats:sub> transport across plasma membranes, but probably via a different pathway than for water.<\/jats:p>","DOI":"10.1111\/pce.12830","type":"journal-article","created":{"date-parts":[[2016,9,13]],"date-time":"2016-09-13T07:46:48Z","timestamp":1473752808000},"page":"789-801","source":"Crossref","is-referenced-by-count":32,"title":["Association between water and carbon dioxide transport in leaf plasma membranes: assessing the role of aquaporins"],"prefix":"10.1111","volume":"40","author":[{"given":"Manchun","family":"Zhao","sequence":"first","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute University of Adelaide  Glen Osmond South Australia 5064 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hwei\u2010Ting","family":"Tan","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute University of Adelaide  Glen Osmond South Australia 5064 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Johannes","family":"Scharwies","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute University of Adelaide  Glen Osmond South Australia 5064 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kara","family":"Levin","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute University of Adelaide  Glen Osmond South Australia 5064 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John R","family":"Evans","sequence":"additional","affiliation":[{"name":"Division of Plant Sciences, Research School of Biology The Australian National University  Canberra Australian Capital Territory 0200 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen D","family":"Tyerman","sequence":"additional","affiliation":[{"name":"Australian Research Council Centre of Excellence in Plant Energy Biology, School of Agriculture, Food and Wine, Waite Research Institute University of Adelaide  Glen Osmond South Australia 5064 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,14]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pch209"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2012.06.013"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-008-0365-1"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erj046"},{"key":"e_1_2_5_6_1","first-page":"219","article-title":"Effects on water diffusion of inhibitors affecting various transport processes in human red\u2010blood\u2010cells","volume":"59","author":"Benga G.","year":"1992","journal-title":"European Journal of Cell Biology"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2007.10.034"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2010.055244"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.201100034"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12832"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.275.6.C1481"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0005-2736(90)90365-U"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2013.00382"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2005.085761"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2008.165027"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.04-3300com"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-8137.1999.00440.x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erp117"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2001.013813"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2012.05.009"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12476"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/ert319"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00421"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1085\/jgp.69.6.779"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2011.04634.x"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-014-0232-7"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.2000.279.6.R1988"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1529\/biophysj.106.081406"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707662104"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.12-209916"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.08.004"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00204582"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2011.11.3413"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcg167"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(84)80502-5"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(94)28046-0"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)48054-3"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.051268"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1139\/v73-140"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00008.2015"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M800096200"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphc.200900270"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.231471998"},{"key":"e_1_2_5_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0813231106"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.1998.274.2.C543"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.1996.271.4.F886"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.115.2.561"},{"key":"e_1_2_5_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)01729-9"},{"key":"e_1_2_5_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(02)03581-0"},{"key":"e_1_2_5_51_1","article-title":"Controls on the emission of plant volatiles through stomata: a sensitivity analysis","volume":"108","author":"Niinemets O.","year":"2003","journal-title":"Journal of Geophysical Research"},{"key":"e_1_2_5_52_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.115881"},{"key":"e_1_2_5_53_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-3495(98)74058-5"},{"key":"e_1_2_5_54_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.109.145326"},{"key":"e_1_2_5_55_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.50.33123"},{"key":"e_1_2_5_56_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20060569"},{"key":"e_1_2_5_57_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2008.02607.x"},{"key":"e_1_2_5_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcf001"},{"key":"e_1_2_5_59_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.0016-8025.2001.00791.x"},{"key":"e_1_2_5_60_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02027"},{"key":"e_1_2_5_61_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.107.054023"},{"key":"e_1_2_5_62_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep00538"},{"key":"e_1_2_5_63_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2011.02473.x"},{"key":"e_1_2_5_64_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.108.128645"},{"key":"e_1_2_5_65_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01870707"},{"key":"e_1_2_5_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12449"},{"key":"e_1_2_5_67_1","doi-asserted-by":"publisher","DOI":"10.1038\/368332a0"},{"key":"e_1_2_5_68_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.15.00637"},{"key":"e_1_2_5_69_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erm245"},{"key":"e_1_2_5_70_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.4.2686"},{"key":"e_1_2_5_71_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2006.11.025"},{"key":"e_1_2_5_72_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00428a062"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpce.12830","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fpce.12830","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.12830","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.12830","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/am-pdf\/10.1111\/pce.12830","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.12830","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T18:31:27Z","timestamp":1694543487000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.12830"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,14]]},"references-count":71,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2017,6]]}},"alternative-id":["10.1111\/pce.12830"],"URL":"https:\/\/doi.org\/10.1111\/pce.12830","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,14]]}}}