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To efficiently activate this cold acclimation, low temperature has to be sensed and processed swiftly, a process that is linked with a transient elimination of microtubules. Here, we address cold\u2010induced microtubules elimination in a grapevine cell line stably expressing a green fluorescent protein fusion of <jats:italic>Arabidopsis<\/jats:italic> TuB6, which allows to follow their response <jats:italic>in<\/jats:italic> <jats:italic>vivo<\/jats:italic> and to quantify this response by quantitative image analysis. We use time\u2010course studies with several specific pharmacological inhibitors and activators to dissect the signalling events acting upstream of microtubules elimination. We find that microtubules disappear within 30\u00a0min after the onset of cold stress. We provide evidence for roles of calcium influx, membrane rigidification, and activation of NAD(P)H oxidase as factors in signal susception and amplification. We further conclude that a G\u2010protein in concert with a phospholipase D convey the signal towards microtubules, whereas calmodulin seems to be not involved. Moreover, activation of jasmonate pathway in response to cold is required for an efficient microtubule response. We summarize our findings in a working model on a complex signalling hub at the membrane\u2010cytoskeleton interphase that assembles the susception, perception and early transduction of cold signals.<\/jats:p>","DOI":"10.1111\/pce.13066","type":"journal-article","created":{"date-parts":[[2017,9,12]],"date-time":"2017-09-12T16:17:23Z","timestamp":1505233043000},"page":"2844-2857","source":"Crossref","is-referenced-by-count":46,"title":["Cold sensing in grapevine\u2014Which signals are upstream of the microtubular \u201cthermometer\u201d"],"prefix":"10.1111","volume":"40","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7023-2219","authenticated-orcid":false,"given":"Lixin","family":"Wang","sequence":"first","affiliation":[{"name":"Molecular Cell Biology, Botanical Institute Karlsruhe Institute of Technology  Fritz\u2010Haber\u2010Weg 4 76131 Karlsruhe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Nick","sequence":"additional","affiliation":[{"name":"Molecular Cell Biology, Botanical Institute Karlsruhe Institute of Technology  Fritz\u2010Haber\u2010Weg 4 76131 Karlsruhe Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,9,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcg097"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2007.02292.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.97.1.182"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80004-1"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2296(08)60043-9"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.1996.tb00387.x"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00442-009-1459-x"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pp.46.060195.000523"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.104.053843"},{"key":"e_1_2_6_11_1","article-title":"Actin as deathly switch? 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