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However, the coupling of the DES1\/H<jats:sub>2<\/jats:sub>S signaling pathways to guard cell movement has not been thoroughly elucidated. The results presented here provide genetic evidence for a physiologically relevant signaling pathway that governs guard cell in situ DES1\/H<jats:sub>2<\/jats:sub>S function in stomatal closure. We discovered that ABA\u2010activated DES1 produces H<jats:sub>2<\/jats:sub>S in guard cells. The impaired guard cell ABA phenotype of the <jats:italic>des1<\/jats:italic> mutant can be fully complemented when DES1\/H<jats:sub>2<\/jats:sub>S function has been specifically rescued in guard cells and epidermal cells, but not mesophyll cells. This research further characterized DES1\/H<jats:sub>2<\/jats:sub>S function in the regulation of LONG HYPOCOTYL1 (HY1, a member of the heme oxygenase family) signaling. ABA\u2010induced <jats:italic>DES1<\/jats:italic> expression and H<jats:sub>2<\/jats:sub>S production are hyper\u2010activated in the <jats:italic>hy1<\/jats:italic> mutant, both of which can be fully abolished by the addition of H<jats:sub>2<\/jats:sub>S scavenger. Impaired guard cell ABA phenotype of <jats:italic>des1\/hy1<\/jats:italic> can be restored by H<jats:sub>2<\/jats:sub>S donors. Taken together, this research indicated that guard cell in situ DES1 function is involved in ABA\u2010induced stomatal closure, which also acts as a pivotal hub in regulating HY1 signaling.<\/jats:p>","DOI":"10.1111\/pce.13685","type":"journal-article","created":{"date-parts":[[2019,11,17]],"date-time":"2019-11-17T05:14:08Z","timestamp":1573967648000},"page":"624-636","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":78,"title":["Abscisic acid\u2010triggered guard cell <scp>l<\/scp>\u2010cysteine <i>desulfhydrase<\/i> function and in situ hydrogen sulfide production contributes to heme oxygenase\u2010modulated stomatal closure"],"prefix":"10.1111","volume":"43","author":[{"given":"Jing","family":"Zhang","sequence":"first","affiliation":[{"name":"Laboratory Center of Life Sciences, College of Life Sciences Nanjing Agricultural University  Nanjing People's Republic of 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