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In Arabidopsis, we determined that phospholipase D\u03b11 (PLD\u03b11) is involved in the stress response to high\u2010magnesium conditions. The T\u2010DNA insertion mutant <jats:italic>pld\u03b11<\/jats:italic> is hypersensitive to increased concentrations of magnesium, exhibiting reduced primary root length and fresh weight. PLD\u03b11 activity increases rapidly after high\u2010Mg<jats:sup>2+<\/jats:sup> treatment, and this increase was found to be dose dependent. Two lines harbouring mutations in the HKD motif, which is essential for PLD\u03b11 activity, displayed the same high\u2010Mg<jats:sup>2+<\/jats:sup> hypersensitivity of <jats:italic>pld\u03b11<\/jats:italic> plants. Moreover, we show that high concentrations of Mg<jats:sup>2+<\/jats:sup> disrupt K<jats:sup>+<\/jats:sup> homeostasis, and that transcription of K<jats:sup>+<\/jats:sup> homeostasis\u2010related genes <jats:italic>CIPK9<\/jats:italic> and <jats:italic>HAK5<\/jats:italic> is impaired in <jats:italic>pld\u03b11<\/jats:italic>. Additionally, we found that the <jats:italic>akt1, hak5<\/jats:italic> double mutant is hypersensitive to high\u2010Mg<jats:sup>2+<\/jats:sup>. We conclude that in Arabidopsis, the enzyme activity of PLD\u03b11 is vital in the response to high\u2010Mg<jats:sup>2+<\/jats:sup> conditions, and that PLD\u03b11 mediates this response partially through regulation of K<jats:sup>+<\/jats:sup> homeostasis.<\/jats:p>","DOI":"10.1111\/pce.13831","type":"journal-article","created":{"date-parts":[[2020,6,25]],"date-time":"2020-06-25T10:52:15Z","timestamp":1593082335000},"page":"2460-2475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Phospholipase D\u03b11 mediates the <scp>high\u2010Mg<sup>2<\/sup><\/scp><sup>+<\/sup> stress response partially through regulation of K<sup>+<\/sup> homeostasis"],"prefix":"10.1111","volume":"43","author":[{"given":"Daniela","family":"Kocourkov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzana","family":"Kr\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0488-7465","authenticated-orcid":false,"given":"P\u0159emysl","family":"Pejchar","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krist\u00fdna","family":"Kroumanov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tereza","family":"Podmanick\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Dan\u011bk","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5675-1706","authenticated-orcid":false,"given":"Jan","family":"Martinec","sequence":"additional","affiliation":[{"name":"Institute of Experimental Botany of the Czech Academy of Sciences  Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,8,9]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcn173"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2005.01408.x"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcs140"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12925"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2017.08.005"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.007385"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/tpj.13503"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-313x.1998.00343.x"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2010.03619.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/ppl.12174"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/jexbot\/48.314.1631"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00025070"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1744-7348.2006.00080.x"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1081\/PLN-100106981"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.119.4.1371"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2010.01366.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.190892"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-811308-0.00011-9"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcu102"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cj.2015.11.003"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2011.50"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1039\/c3mt20223b"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.04366"},{"key":"e_1_2_8_25_1","first-page":"1","article-title":"The water\u2010culture method for growing plants without soil","volume":"347","author":"Hoagland D. 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