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This integrative study investigated the impacts of mild, moderate, and severe P\u2010stress on genome transcription and whole\u2010plant metabolism, physiology and development in switchgrass. P\u2010limitation reduced overall plant growth, increased root\/shoot ratio, increased root branching at moderate P\u2010stress, and decreased root diameter with increased density and length of root hairs at severe P\u2010stress. RNA\u2010seq analysis revealed thousands of genes that were differentially expressed under moderate and severe P\u2010stress in roots and\/or shoots compared to P\u2010replete plants, with many stress\u2010induced genes involved in transcriptional and other forms of regulation, primary and secondary metabolism, transport, and other processes involved in P\u2010acquisition and homeostasis. Amongst the latter were multiple miRNA399 genes and putative targets of these. Metabolite profiling showed that levels of most sugars and sugar alcohols decreased with increasing P stress, while organic and amino acids increased under mild and moderate P\u2010stress in shoots and roots, although this trend reversed under severe P\u2010stress, especially in shoots.<\/jats:p>","DOI":"10.1111\/pce.13872","type":"journal-article","created":{"date-parts":[[2020,8,21]],"date-time":"2020-08-21T14:37:02Z","timestamp":1598020622000},"page":"186-202","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["Transcriptional, metabolic, physiological and developmental responses of switchgrass to phosphorus limitation"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1035-0368","authenticated-orcid":false,"given":"Na","family":"Ding","sequence":"first","affiliation":[{"name":"Noble Research Institute LLC  Ardmore Oklahoma 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