{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:28:50Z","timestamp":1787970530665,"version":"build-2784847793"},"reference-count":71,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,28]],"date-time":"2020-12-28T00:00:00Z","timestamp":1609113600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004869","name":"Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004869","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Flavonoids may mediate UV protection in plants either by screening of harmful radiation or by minimizing the resulting oxidative stress. To help distinguish between these alternatives, more precise knowledge of flavonoid distribution is needed. We used confocal laser scanning microscopy (cLSM) with the \u201cemission fingerprinting\u201d feature to study the cellular and subcellular distribution of flavonoid glucosides in the giant duckweed (<jats:italic>Spirodela polyrhiza<\/jats:italic>), and investigated the fitness effects of these compounds under natural UV radiation and copper sulphate addition (oxidative stress) using common garden experiments indoors and outdoors. cLSM \u201cemission fingerprinting\u201d allowed us to individually visualize the major dihydroxylated B\u2010ring\u2010substituted flavonoids, luteolin 7\u2010O\u2010glucoside and luteolin 8\u2010C\u2010glucoside, in cross\u2010sections of the photosynthetic organs. While luteolin 8\u2010C\u2010glucoside accumulated mostly in the vacuoles and chloroplasts of mesophyll cells, luteolin 7\u2010O\u2010glucoside was predominantly found in the vacuoles of epidermal cells. In congruence with its cellular distribution, the mesophyll\u2010associated luteolin 8\u2010C\u2010glucoside increased plant fitness under copper sulphate addition but not under natural UV light treatment, whereas the epidermis\u2010associated luteolin 7\u2010O\u2010glucoside tended to increase fitness under both stresses across chemically diverse genotypes. Taken together, we demonstrate that individual flavonoid glucosides have distinct cellular and subcellular locations and promote duckweed fitness under different abiotic stresses.<\/jats:p>","DOI":"10.1111\/pce.13974","type":"journal-article","created":{"date-parts":[[2020,12,10]],"date-time":"2020-12-10T23:03:22Z","timestamp":1607641402000},"page":"900-914","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":44,"title":["Differential localization of flavonoid glucosides in an aquatic plant implicates different functions under abiotic stress"],"prefix":"10.1111","volume":"44","author":[{"given":"Laura","family":"B\u00f6ttner","sequence":"first","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"},{"name":"Institute of Plant Biology and Biotechnology University of M\u00fcnster  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veit","family":"Grabe","sequence":"additional","affiliation":[{"name":"Department of Evolutionary Neuroethology Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saskia","family":"Gablenz","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Niklas","family":"B\u00f6hme","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Klaus J.","family":"Appenroth","sequence":"additional","affiliation":[{"name":"Matthias\u2010Schleiden\u2010Institute, Plant Physiology Friedrich Schiller University  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Gershenzon","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8708-394X","authenticated-orcid":false,"given":"Meret","family":"Huber","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Max\u2010Planck\u2010Institute for Chemical Ecology  Jena Germany"},{"name":"Institute of Plant Biology and Biotechnology University of M\u00fcnster  M\u00fcnster Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,12,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plantsci.2012.07.014"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jplph.2010.07.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.plaphy.2013.03.014"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1562\/0031-8655(2002)0760350FDITOP2.0.CO2"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2007.01986.x"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/aob\/mcp177"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02879642"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/pcp\/pcg160"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12609"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.18637\/jss.v067.i01"},{"key":"e_1_2_7_12_1","first-page":"257","article-title":"Magnesium, potassium flux and photosynthesis","volume":"6","author":"Berkowitz G.","year":"1993","journal-title":"Mangesium Research"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jphotobiol.2007.06.002"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10750-014-2163-3"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj3301173"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2018.01042"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.107.101824"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3040.2000.00633.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1021\/jf001413m"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1983.tb04206.x"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00440"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009229429250"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00039"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2013.09.012"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(02)00712-4"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02858564"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erq458"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.3390\/f5092122"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fob.2015.08.009"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/erh092"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envexpbot.2010.08.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)00235-1"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tplants.2008.12.003"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1002\/bimj.200810425"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jxb\/49.323.953"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.2012.01656.x"},{"key":"e_1_2_7_37_1","first-page":"311","article-title":"Effect of flavonoids on heavy metal tolerance in Arabidopsis thaliana seedlings","volume":"50","author":"Keilig K.","year":"2009","journal-title":"Botanical Studies"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3040.2004.01180.x"},{"key":"e_1_2_7_39_1","first-page":"271","article-title":"Physiologische und \u00f6kologische Untersuchungen an Lemnaceen","volume":"67","author":"Landolt E.","year":"1957","journal-title":"Berichte der Schweizerischen Botanischen Gesellschaft"},{"key":"e_1_2_7_40_1","volume-title":"The family of Lemnaceae\u2014A monographic study., Vol 1","author":"Landolt E.","year":"1986"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1105\/tpc.110.074161"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-3054.1995.tb05123.x"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.2298\/JSC0710921M"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00706-011-0545-z"},{"key":"e_1_2_7_45_1","first-page":"523","article-title":"Phenolic compounds and their antioxidant activity in plants growing under heavy metal stress","volume":"15","author":"Michalak A.","year":"2006","journal-title":"Polish Journal of Environmental Studies"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1080\/1071576021000016463"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2014.00620"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.20307\/nps.2016.22.1.1"},{"key":"e_1_2_7_49_1","first-page":"533","article-title":"Anthocyanins: Possible antioxidants against Cr (VI) induced oxidative stress in Spirodela polyrrhiza","volume":"37","author":"Ol\u00e1h V.","year":"2009","journal-title":"Cereal Research Communications"},{"key":"e_1_2_7_50_1","first-page":"181","article-title":"Comparative study on sensitivity of different physiological properties of Spirodela polyrrhiza (L.) Schleiden to Cr (VI) treatments","volume":"52","author":"Ol\u00e1h V.","year":"2008","journal-title":"Acta Biologica Szegediensis"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1017\/jns.2016.41"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1104\/pp.126.2.536"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.3389\/fpls.2016.00601"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.1002\/pca.1303"},{"key":"e_1_2_7_55_1","volume-title":"R: A Language and Environment for Statistical Computing","author":"R Core Team","year":"2016"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1011-1344(01)00206-8"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1016\/0891-5849(95)02227-9"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1111\/pce.12374"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)94452-2"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.1996.tb01844.x"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4705"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac00055a022"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.1016\/0031-9422(88)80759-3"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11738-012-1169-6"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20035024"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-8137.2004.01126.x"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1515\/znc-1991-1-207"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0031-9422(00)85800-8"},{"key":"e_1_2_7_69_1","first-page":"8","article-title":"Low genetic variation is associated with low mutation rate in the giant duckweed","volume":"10","author":"Xu S.","year":"2019","journal-title":"Nature Communications"},{"key":"e_1_2_7_70_1","first-page":"54199","article-title":"The reduction effect of dietary flavone C\u2010 and O\u2010glycosides on the formation of acrylamide and its correlation and prediction with the antioxidant activity of Maillard reaction products","volume":"4","author":"Zhang Y.","year":"2014","journal-title":"The Royal Society of Chemistry"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11103-012-9889-y"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1111\/plb.12184"}],"container-title":["Plant, Cell &amp; Environment"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.13974","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/pce.13974","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/pce.13974","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,3]],"date-time":"2023-09-03T02:43:33Z","timestamp":1693709013000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/pce.13974"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,28]]},"references-count":71,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2021,3]]}},"alternative-id":["10.1111\/pce.13974"],"URL":"https:\/\/doi.org\/10.1111\/pce.13974","archive":["Portico"],"relation":{},"ISSN":["0140-7791","1365-3040"],"issn-type":[{"value":"0140-7791","type":"print"},{"value":"1365-3040","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,28]]},"assertion":[{"value":"2018-11-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}