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Plants at the reproductive stage are especially susceptible to heat stress due to damage to male (anthers) and female (ovary) reproductive tissues, with anthers being more sensitive to heat. Heat damage promotes early tapetal cell degradation, and in beans this was shown to cause male infertility. In this study, we focus on understanding how changes in leaf carbon export in response to elevated temperature stress contribute to heat\u2010induced infertility. We hypothesize that anther glucose\u20106\u2010phosphate dehydrogenase (G6PDH) activity plays an important role at elevated temperature and promotes thermotolerance. To test this hypothesis, we compared heat\u2010tolerant and susceptible common bean genotypes using a combination of phenotypic, biochemical, and physiological approaches. Our results identified changes in leaf sucrose export, anther sugar accumulation and G6PDH activity and anther H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> levels and antioxidant\u2010related enzymes between genotypes at elevated temperature. Further, anther respiration rate was found to be lower at high temperature in both bean varieties. Overall, our results support the hypothesis that enhanced male reproductive heat tolerance involves changes in the anther oxidative pentose phosphate pathway, which supplies reductants to critical H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> scavenging enzymes.<\/jats:p>","DOI":"10.1111\/pce.14057","type":"journal-article","created":{"date-parts":[[2021,3,30]],"date-time":"2021-03-30T14:28:23Z","timestamp":1617114503000},"page":"2185-2199","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":30,"title":["Contrasting anther glucose\u20106\u2010phosphate dehydrogenase activities between two bean varieties suggest an important role in reproductive heat tolerance"],"prefix":"10.1111","volume":"44","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4186-8303","authenticated-orcid":false,"given":"James P.","family":"Santiago","sequence":"first","affiliation":[{"name":"Michigan State University\u2010Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan USA"},{"name":"Plant Resilience Institute, Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0951-561X","authenticated-orcid":false,"given":"Ali","family":"Soltani","sequence":"additional","affiliation":[{"name":"Department of Plant Biology Michigan State University  East Lansing Michigan USA"},{"name":"Plant Resilience Institute, Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madeline M.","family":"Bresson","sequence":"additional","affiliation":[{"name":"Michigan State University\u2010Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alyssa L.","family":"Preiser","sequence":"additional","affiliation":[{"name":"Michigan State University\u2010Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan USA"},{"name":"Department of Biochemistry and Molecular Biology Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David B.","family":"Lowry","sequence":"additional","affiliation":[{"name":"Department of Plant Biology Michigan State University  East Lansing Michigan USA"},{"name":"Plant Resilience Institute, Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4423-3223","authenticated-orcid":false,"given":"Thomas D.","family":"Sharkey","sequence":"additional","affiliation":[{"name":"Michigan State University\u2010Department of Energy Plant Research Laboratory Michigan State University  East Lansing Michigan USA"},{"name":"Department of Biochemistry and Molecular Biology Michigan State University  East Lansing Michigan USA"},{"name":"Plant Resilience Institute, Michigan State University  East Lansing Michigan USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,5,2]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.aob.a088170"},{"key":"e_1_2_10_3_1","first-page":"129","article-title":"Enhanced tolerance to photooxidative stress of transgenic Nicotiana tabacum with high chloroplastic glutathione reductase activity","volume":"34","author":"Aono M.","year":"1993","journal-title":"Plant Cell Physiology"},{"key":"e_1_2_10_4_1","first-page":"55","volume-title":"Export of photosynthates from the leaf","author":"Ayre B. 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