{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:44:05Z","timestamp":1785545045740,"version":"3.56.0"},"reference-count":73,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,4,18]],"date-time":"2022-04-18T00:00:00Z","timestamp":1650240000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Glutathione (GSH) is known to regulate iron (Fe) deficiency response in plants but its involvement in modulating subcellular Fe homoeostasis remains elusive. In this study, we report that the GSH\u2010deficient mutants,\n                    <jats:italic>cad2\u20101<\/jats:italic>\n                    and\n                    <jats:italic>pad2\u20101<\/jats:italic>\n                    displayed increased sensitivity to Fe deficiency with significant downregulation of the vacuolar Fe exporters,\n                    <jats:italic>AtNRAMP3<\/jats:italic>\n                    and\n                    <jats:italic>AtNRAMP4<\/jats:italic>\n                    , and the chloroplast Fe importer,\n                    <jats:italic>AtPIC1<\/jats:italic>\n                    . Moreover, the\n                    <jats:italic>pad2\u20101<\/jats:italic>\n                    mutant accumulated higher Fe levels in vacuoles but lower Fe levels in chloroplasts compared to wild type (Columbia ecotype [Col\u20100]) under Fe limited conditions. Exogenous GSH treatment enhanced chloroplast Fe contents in Col\u20100 but failed to do so in the\n                    <jats:italic>nramp3nramp4<\/jats:italic>\n                    double mutants demonstrating that GSH plays a role in modulating subcellular Fe homoeostasis. Pharmacological experiments, mutant analysis, and promoter assays revealed that this regulation involves the transcriptional activation of Fe transporter genes by a GSH\u2013\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosoglutathione\u00a0(GSNO) module. The Fe responsive bHLH\u00a0transcription factors (TFs),\n                    <jats:italic>At<\/jats:italic>\n                    bHLH29,\n                    <jats:italic>At<\/jats:italic>\n                    bHLH38, and\n                    <jats:italic>At<\/jats:italic>\n                    bHLH101 were found to interact with the promoters of these genes, which were, in turn, activated via\n                    <jats:italic>S<\/jats:italic>\n                    \u2010nitrosylation (SNO). Taken together, the present study highlights the role of the GSH\u2013GSNO module in regulating subcellular Fe homoeostasis by transcriptional activation of the Fe transporters\n                    <jats:italic>AtNRAMP3<\/jats:italic>\n                    ,\n                    <jats:italic>AtNRAMP4<\/jats:italic>\n                    , and\n                    <jats:italic>AtPIC1<\/jats:italic>\n                    via SNO of bHLH TFs during Fe deficiency.\n                  <\/jats:p>","DOI":"10.1111\/pce.14331","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T11:24:11Z","timestamp":1649417051000},"page":"2176-2190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Glutathione regulates transcriptional activation of iron transporters via\n                    <i>S<\/i>\n                    \u2010nitrosylation of bHLH factors to modulate subcellular iron homoeostasis"],"prefix":"10.1111","volume":"45","author":[{"given":"Ranjana","family":"Shee","sequence":"first","affiliation":[{"name":"Department of Botany Dr. A. 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