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Polyamine levels change in response to different pathogens, including <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato<\/jats:italic> DC3000 (<jats:italic>Pst<\/jats:italic> DC3000). However, the regulation of polyamine metabolism and their specific contributions to defence are not fully understood. Here we report that stimulation of Put biosynthesis by <jats:italic>Pst<\/jats:italic> DC3000 is dependent on coronatine (COR) perception and jasmonic acid (JA) signalling, independently of salicylic acid (SA). Conversely, lack of Spm in <jats:italic>spermine synthase<\/jats:italic> (<jats:italic>spms<\/jats:italic>) mutant stimulated galactolipids and JA biosynthesis, and JA signalling under basal conditions and during <jats:italic>Pst<\/jats:italic> DC3000 infection, whereas compromised SA\u2010pathway activation and defence outputs through SA\u2013JA antagonism. The dampening of SA responses correlated with COR and <jats:italic>Pst<\/jats:italic> DC3000\u2010inducible deregulation of <jats:italic>ANAC019<\/jats:italic> expression and its key SA\u2010metabolism gene targets. Spm deficiency also led to enhanced disease resistance to the necrotrophic fungal pathogen <jats:italic>Botrytis cinerea<\/jats:italic> and stimulated endoplasmic reticulum (ER) stress signalling in response to <jats:italic>Pst<\/jats:italic> DC3000. Overall, our findings provide evidence for the integration of polyamine metabolism in JA\u2010 and SA\u2010mediated defence responses, as well as the participation of Spm in buffering ER stress during defence.<\/jats:p>","DOI":"10.1111\/pce.14706","type":"journal-article","created":{"date-parts":[[2023,8,31]],"date-time":"2023-08-31T18:55:02Z","timestamp":1693508102000},"page":"3949-3970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Spermine deficiency shifts the balance between jasmonic acid and salicylic acid\u2010mediated defence responses in <i>Arabidopsis<\/i>"],"prefix":"10.1111","volume":"46","author":[{"given":"Chi","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Biology, Healthcare and Environment, Section of Plant Physiology, Faculty of Pharmacy and Food Sciences Universitat de Barcelona Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kostadin 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