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The <jats:italic>noxy8<\/jats:italic> mutation was located at the <jats:italic>CLPC2<\/jats:italic> gene, encoding a chloroplast chaperone of the protease complex CLP. Although its CLPC1 paralogue is considered to generate redundancy, our data reveal significant differences distinguishing CLPC2 and CLPC1 functions. As such, <jats:italic>clpc1<\/jats:italic> mutants displayed a major defect in housekeeping chloroplast proteostasis, leading to a pronounced reduction in growth and pigment levels, enhanced accumulation of chloroplast and cytosol chaperones, and resistance to fosmidomycin. Conversely, <jats:italic>clpc2<\/jats:italic> mutants showed severe susceptibility to lincomycin inhibition of chloroplast translation and resistance to Antimycin A inhibition of mitochondrial respiration. In the response to <jats:italic>Pseudomonas syringae<\/jats:italic> pv. <jats:italic>tomato, clpc2<\/jats:italic> but not <jats:italic>clpc1<\/jats:italic> mutants were resistant to bacterial infection, showing higher salicylic acid levels, defence gene expression and 9\u2010LOX pathway activation. Our findings suggest CLPC2 and CLPC1 functional specificity, with a preferential involvement of CLPC1 in housekeeping processes and of CLPC2 in stress responses.<\/jats:p>","DOI":"10.1111\/pce.14882","type":"journal-article","created":{"date-parts":[[2024,3,19]],"date-time":"2024-03-19T07:21:35Z","timestamp":1710832895000},"page":"2334-2348","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Mutant <i>noxy8<\/i> exposes functional specificities between the chloroplast chaperones CLPC1 and CLPC2 in the response to organelle stress and plant defence"],"prefix":"10.1111","volume":"47","author":[{"given":"Bran","family":"L\u00f3pez","sequence":"first","affiliation":[{"name":"Centro Nacional de Biotecnolog\u00eda (CNB\u2010CSIC), Cantoblanco Madrid Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yovanny","family":"Izquierdo","sequence":"additional","affiliation":[{"name":"Centro Nacional de 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