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However, the mechanism through which AB remains dormant or emerges to form branches as plants grow remains largely unknown. Here, the auxin\u2010strigolactone (IAA\u2010SL) pathway was first shown to regulate shoot branching in poplar, and we found that <jats:italic>PagKNAT2\/6b<\/jats:italic> could modulate this pathway. <jats:italic>PagKNAT2\/6b<\/jats:italic> was expressed mainly in the shoot apical meristem and AB and was induced by shoot apex damage. <jats:italic>PagKNAT2\/6b<\/jats:italic> overexpressing poplar plants (<jats:italic>PagKNAT2\/6b<\/jats:italic> OE) exhibited multiple branches that mimicked the branching phenotype of nontransgenic plants after decapitation treatment, while compared with nontransgenic controls, <jats:italic>PagKNAT2\/6b<\/jats:italic> antisense transgenic poplar and <jats:italic>Pagknat2\/6b<\/jats:italic> mutant lines exhibited a significantly decreased number of branches after shoot apex damage treatment. In addition, we found that PagKNAT2\/6b directly inhibits the expression of the key IAA synthesis gene <jats:italic>PagYUC6a<\/jats:italic>, which is specifically expressed in the shoot apex. Moreover, overexpression of <jats:italic>PagYUC6a<\/jats:italic> in the <jats:italic>PagKNAT2\/6b<\/jats:italic> OE background reduced the number of branches after shoot apex damage treatment. Overall, we conclude that <jats:italic>PagKNAT2\/6b<\/jats:italic> responds to shoot apical injury and regulates shoot branching through the IAA\u2010SL pathway. These findings may provide a theoretical basis and candidate genes for genetic engineering to create new forest tree species with different crown types.<\/jats:p>","DOI":"10.1111\/pce.14991","type":"journal-article","created":{"date-parts":[[2024,6,7]],"date-time":"2024-06-07T11:15:59Z","timestamp":1717758959000},"page":"3920-3935","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["<i>PagKNAT2\/6b<\/i> promotes shoot branching by attenuating auxin\u2010strigolactone signalling in poplar"],"prefix":"10.1111","volume":"47","author":[{"given":"Yan\u2010Qiu","family":"Zhao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Subtropical Silviculture Zhejiang A&amp;F University, Key Laboratory of Modern Silvicultural Technology of Zhejiang Province Hangzhou Zhejiang China"},{"name":"The Engineering Research, Institute of Agriculture and Forestry Ludong University Yantai Shandong 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