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However, its involvement in the response to abiotic stresses remains unclear. Our study aimed to elucidate this mechanism by predicting the targeting of the rice P\u2010type IIB Ca<jats:sup>2+<\/jats:sup> ATPase gene <jats:italic>OsACAs<\/jats:italic> by miR1432 and identifying its cleavage sites via 5\u2032RACE. We observed induced expression of miR1432 and its target gene, <jats:italic>OsACA6<\/jats:italic>, under abiotic stresses. Overexpression (OX) of miR1432 and suppression of <jats:italic>OsACA6<\/jats:italic> resulted in reduced cold, salt, and drought tolerance, while <jats:italic>OsACA6<\/jats:italic> suppression\/knockout and OX had opposite effects on cold tolerance. Additionally, miR1432 may target other <jats:italic>OsACA6<\/jats:italic> homologs. RNA\u2010sequencing data highlighted the differential expression of stress\u2010related genes in miR1432\u2010overexpressing rice. Furthermore, miR1432\u2010overexpressing rice exhibited weakened vigor, dwarfism, yellowing leaves and reduced fertility. Collectively, our results strongly suggest that miR1432 not only negatively modulates abiotic stress tolerance by suppressing Ca<jats:sup>2+<\/jats:sup> ATPase gene(s) but also influences plant growth and development.<\/jats:p>","DOI":"10.1111\/pce.15109","type":"journal-article","created":{"date-parts":[[2024,8,27]],"date-time":"2024-08-27T14:15:23Z","timestamp":1724768123000},"page":"5443-5456","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["miR1432 negatively regulates cold tolerance by targeting <i>OsACAs<\/i>"],"prefix":"10.1111","volume":"47","author":[{"given":"Yan","family":"Dai","sequence":"first","affiliation":[{"name":"Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology Chinese Academy of Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiujing","family":"Feng","sequence":"additional","affiliation":[{"name":"Key Laboratory of Seed 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