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Root and stem rot diseases caused by the necrotrophic pathogen <jats:italic>Heterobasidion parviporum<\/jats:italic> threaten the wood production of Norway spruce which necessitates the search for durable control and management strategies. Breeding for resistant traits is considered a viable long\u2010term strategy. However, identification of potential resistant traits and markers remains a major challenge. In this study, short\u2010term disease resistance screening was conducted using 218 Norway spruce clones from 17 families. Disease resistance was evaluated based on the size of necrosis lesion length following infection with the pathogen. A subset of needles\/branches from clones with small (partial resistant) or large (susceptible) lesions were used for terpene analysis and transcriptomic profiling. The results revealed that the content of monoterpene linalool and 1,8\u2010cineole and their respective encoded genes were significantly more abundant and highly expressed in the partial resistant group. Furthermore, linalool and 1,8\u2010cineole were demonstrated to have inhibitory effect on the growth of the pathogen <jats:italic>H. parviporum<\/jats:italic>, with morphological distortion of the hyphae. RNAseq analysis revealed that transcript of pathogen genes involved in the regulation of carbohydrate metabolism and stress responses were significantly decreased in presence of the terpenes. The results suggest the relevance of monoterpenes together with jasmonic acid precursor and some genes involved in phenylpropanoid biosynthesis, as constitutive tolerance factors for Norway spruce tolerance against necrotrophic pathogen. The high level of necrosis related cell death gene expression might be factors critical for host susceptibility and disease development.<\/jats:p>","DOI":"10.1111\/pce.15280","type":"journal-article","created":{"date-parts":[[2024,11,13]],"date-time":"2024-11-13T11:54:38Z","timestamp":1731498878000},"page":"1993-2008","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Linalool and 1,8\u2010Cineole as Constitutive Disease\u2010Resistant Factors of Norway Spruce Against Necrotrophic Pathogen <i>Heterobasidion Parviporum<\/i>"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5205-7346","authenticated-orcid":false,"given":"Kai","family":"Wang","sequence":"first","affiliation":[{"name":"College of Forestry Fujian Agriculture and Forestry University Fuzhou China"},{"name":"Department of Forest Sciences University of Helsinki Helsinki 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