{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T18:04:33Z","timestamp":1788717873960,"version":"build-2803163510"},"reference-count":41,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,11,21]],"date-time":"2024-11-21T00:00:00Z","timestamp":1732147200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:italic>Sedum plumbizincicola<\/jats:italic> (<jats:italic>Sp<\/jats:italic>) is a cadmium (Cd) hyperaccumulator found specifically in abandoned ancient mines where <jats:italic>N<\/jats:italic> is regularly deficient while Cd presents in excess. How <jats:italic>Sp<\/jats:italic> got adapted to this unique habitat remains unknown. Here, we reported relative abundant presence of NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> in mine areas for <jats:italic>Sp<\/jats:italic>, and the isolation and functional characterisation of a putative NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> transporter gene <jats:italic>AMT1;2<\/jats:italic>, which is highly expressed in <jats:italic>Sp<\/jats:italic> roots and encodes a pH\u2010dependent dual affinity ammonium uptake transporter. Compared to <jats:italic>SaAMT1;2<\/jats:italic>, the homologous gene in the nonhyperaccumulating control <jats:italic>Sedum alfredii<\/jats:italic> (<jats:italic>Sa<\/jats:italic>), <jats:italic>SpAMT1;2<\/jats:italic> expression is much higher and not inhibited by Cd. Only eight amino acid sequence polymorphisms were observed between SpAMT1;2 and SaAMT1;2, and the in\u2010vitro NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> uptake activity and subcellular localisation are identical between them with or without Cd stress. Moreover, in contrast in <jats:italic>Sa<\/jats:italic>, NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> uptake in <jats:italic>Sp<\/jats:italic> is not inhibited by Cd, and NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup> at ambient level promotes Cd accumulation. These data suggest that <jats:italic>SpAMT1;2<\/jats:italic> is likely an essential gene contributing to nitrogen nutrition and the interaction between NH<jats:sub>4<\/jats:sub><jats:sup>+<\/jats:sup>and Cd uptake in <jats:italic>Sp<\/jats:italic>, which might represent a novel N utilisation pathway evolved in mines for the hyperaccumulator <jats:italic>Sp<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/pce.15296","type":"journal-article","created":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T05:17:31Z","timestamp":1732252651000},"page":"2256-2266","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The Ammonium Transporter <i>SpAMT1;2<\/i> Contributes to Nitrogen Utilisation and Cadmium Accumulation in the Hyperaccumulator <i>Sedum Plumbizincicola<\/i>"],"prefix":"10.1111","volume":"48","author":[{"given":"Yan\u2010Xuan","family":"Yu","sequence":"first","affiliation":[{"name":"National Key Laboratory of Molecular Plant Genetics, Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng\u2010Qi","family":"Wang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Molecular Plant Genetics, Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Jun","family":"Fang","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Molecular Plant Genetics, Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9312-1607","authenticated-orcid":false,"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Molecular Plant Genetics, Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010Ming","family":"Gong","sequence":"additional","affiliation":[{"name":"National Key Laboratory of Molecular Plant Genetics, Center for Plant Stress Biology, CAS Center for Excellence in Molecular Plant Sciences Chinese Academy of Sciences Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,11,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1080\/15592324.2019.1625696"},{"key":"e_1_2_8_3_1","first-page":"585","article-title":"Advances in Mineral Element Interactions and Heavy Metal Pollution","volume":"50","author":"Chen J. 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