{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:33:36Z","timestamp":1787506416611,"version":"build-2736575974"},"reference-count":60,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2024,12,15]],"date-time":"2024-12-15T00:00:00Z","timestamp":1734220800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Plant Cell &amp; Environment"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Photorespiration is a complex metabolic process linked to primary plant metabolism and influenced by environmental factors, yet its regulation remains poorly understood. In this study, we identified the\n                    <jats:italic>asprs3\u20101<\/jats:italic>\n                    mutant, which displays a photorespiratory phenotype with leaf chlorosis, stunted growth, and diminished photosynthesis under ambient CO\n                    <jats:sub>2<\/jats:sub>\n                    , but normal growth under elevated CO\n                    <jats:sub>2<\/jats:sub>\n                    conditions. Map\u2010based cloning and genetic complementation identified\n                    <jats:italic>AspRS3<\/jats:italic>\n                    as the mutant gene, encoding an aspartyl\u2010tRNA synthetase. AspRS3 is localised in both chloroplasts and mitochondria, with the chloroplast being the primary site of its physiological function. The AspRS3 mutation impacts the expression of plastid\u2010encoded and photosynthesis\u2010related genes, leading to decreased levels of chloroplast\u2010encoded proteins such as ribulose\u20101,5\u2010bisphosphate carboxylase\/oxygenase large subunit (RBCL) and ferredoxin\u2010dependent glutamate synthase (Fd\u2010GOGAT). Furthermore, we observed an accumulation of photorespiratory intermediates, including glycine and glycerate, and reactive oxygen species (ROS) in\n                    <jats:italic>asprs3\u20101<\/jats:italic>\n                    . However, under high CO\n                    <jats:sub>2<\/jats:sub>\n                    , the expression of these proteins, the accumulation of photorespiratory intermediates, and ROS levels in\n                    <jats:italic>asprs3\u20101<\/jats:italic>\n                    did not significantly differ from those in the wild type. We propose that elevated CO\n                    <jats:sub>2<\/jats:sub>\n                    mitigates the\n                    <jats:italic>asprs3\u20101<\/jats:italic>\n                    phenotype by inhibiting Rubisco oxygenation and photorespiratory metabolism. This study highlights the role of aminoacyl\u2010tRNA synthetases in regulating photorespiration and provides new insights into its metabolic control.\n                  <\/jats:p>","DOI":"10.1111\/pce.15330","type":"journal-article","created":{"date-parts":[[2024,12,16]],"date-time":"2024-12-16T02:45:01Z","timestamp":1734317101000},"page":"2998-3011","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Chloroplastic Aspartyl\u2010tRNA Synthetase Is Required for Chloroplast Development, Photosynthesis and Photorespiratory Metabolism"],"prefix":"10.1111","volume":"48","author":[{"given":"Yue","family":"Xi","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory for the Development Biology and Environmental Adaptation of Agricultural Organisms, College of Life Sciences South China Agricultural University Guangzhou 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