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Studies have indicated that <jats:italic>N<\/jats:italic><jats:sup>6<\/jats:sup>\u2010methyladenosine (m<jats:sup>6<\/jats:sup>A) reader regulates plant stress response by binding to their corresponding mRNA targets with m<jats:sup>6<\/jats:sup>A modification. The present study investigated the function and mechanism of apple m<jats:sup>6<\/jats:sup>A reader MhYTP2 under hypoxia stress. Here, we found that the overexpression of <jats:italic>MhYTP2<\/jats:italic> improved hypoxia resistance in apple. Previous RNA immunoprecipitation sequencing (RIP\u2010seq) results identified the mRNA of Ethylene Response Factor 54\u00a0(ERF54) as a direct target of MhYTP2; electronic mobility shift assays (EMSA) further verified this finding. Further transcription inhibition assays demonstrated that MhYTP2 increased <jats:italic>MdERF54<\/jats:italic> mRNA stability. Under hypoxia stress, <jats:italic>MdERF54<\/jats:italic> increased the activities of pyruvate decarboxylase (PDC), lactate dehydrogenase (LDH), and alcohol dehydrogenase (ADH) the key enzymes in anaerobic respiration pathway, activated the ethylene signalling pathway, increased the chlorophyll content of plant leaves and photosynthetic rates, enhanced the adaptability of roots, reduced the damage to biofilm and antioxidant system, and enhanced the antioxidant capacity. Thus, our results elucidated the molecular mechanisms by which the MhYTP2\u2013<jats:italic>MdERF54<\/jats:italic> module influences the response of the apple to hypoxia stress.<\/jats:p>","DOI":"10.1111\/pce.15527","type":"journal-article","created":{"date-parts":[[2025,4,8]],"date-time":"2025-04-08T05:44:05Z","timestamp":1744091045000},"page":"5383-5398","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["The m<sup>6<\/sup>A Reader MhYTP2 Regulates <i>MdERF54<\/i> mRNA Stability and Contributes to Hypoxia Tolerance in Apple (<i>Malus domestica<\/i>)"],"prefix":"10.1111","volume":"48","author":[{"given":"Tianli","family":"Guo","sequence":"first","affiliation":[{"name":"State Key Laboratory for Crop Stress Resistance and High\u2010Efficiency Production\/Shaanxi Key Laboratory of Apple, College of Horticulture Northwest A&amp;F University Yangling Shaanxi China"},{"name":"State Key Laboratory for Conservation and 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